Team:GZHS-United/InterLab

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InterLab

Calibration

Calibration 1: Find OD600 reference point using LUDOX and ddH2O.
Calibration 2: Create a particle standard curve using microsphere stock solution.
Calibration 3: Create a fluorescence standard curve using fluorescein.

Cell measurment

1. Transform the 8 plasmids (Positive & Negative Control, Test Devices 1-6) into E.coli DH5α.
2. Pick colonies and inoculate LB medium + Chloramphenicol. Grow the cells overnight.
3. Dilute each overnight culture in LB+Chloramphenicol and measure Abs600 of these diluted cultures
4. Dilute the cultures further to a target Abs600 of 0.02 in a final volume of 12 ml LB medium + Chloramphenicol.
5. Take 500 µL samples of the diluted cultures at 0 hours and measure each samples’ Abs600 and fluorescence measurement.
6. Incubate the remainder of the cultures at 37°C and 220 rpm for 6 hours.
7. Measure each samples’ Abs600 and fluorescence measurement at 6 hours.
8. Find the colony forming units per 0.1 OD600 E. coli cultures.

Data

OD600 reference point

	LUDOX CL-X	H2O
Replicate 1	0.063	0.038
Replicate 2	0.065	0.040
Replicate 3	0.069	0.037
Replicate 4	0.067	0.045
Arith. Mean	0.066	0.040
Corrected Abs600	0.026
Reference OD600	0.063
OD600/Abs600	2.423

Particle Standrad Curve

Fluorescence Standard Curve

For more information, click here