Team:Uppsala/Protocol

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Below you can find the protocols we have been using when conducting our experiments in the lab.

Worm Culturing

• Co-culturing protocol
• Nematode recovery from cups protocol
• Worm purification protocol
• Worm separation system protocol
• Worm sterilization protocol

Transcriptomics

• TotalRNA purification protocol
• rRNA depletion protocol
• Poly(A)-tailing protocol
• cDNA conversion protocol
• Library/End Prep protocol
• OD600 and CFU count protocol
• RNA quality check

Reporter System

• UnaG Transformation and Extraction
• Simple Chromoprotein Visibility Analysis Protocol
• Horse Feces Analysis Using a Plate Reader
• Making CaCl₂ competent E.coli cells

Phage Display

• Panning/titering/amplification
• Phage dna for sequencing
• Original protocol supplied by New England Biolabs

Improve AmilCP

• 3A Assembly Protocol
• Agarose Gel Electrophoresis Protocol
• Single Tube Transformation Protocol
• Liquid Experiment, Stability Assay Protocol