Team:Botchan Lab Tokyo/Safety
Safety
<Our Project>
What will your project work?
-It will be engineered to do nitrogen fixation and convert nitrogen gas into ammonia and eventually amino acids. We will make this mechanism in Escherichia coli.
Please describe any other organisms besides your host cell you will use ?
-We will use E. coli JM109 for our host cell. We are going to put nifBHDKENX-hesA from Paenibacillus polymyxa, and nifVSU from Rahnella aqatilis to synthesize nitrogenase for nitrogen fixation. We also introduce phenylalanine dehydrogenase genes from Bacillus badius, and glutamate dehydrogenase genes from Bacillus licheniformis for the synthesis of amino acids. These three organisms are Risk Group 1 organisms.
*Source Link
Paenibacillus polymyxa :https://www.atcc.org/products/all/15970.aspx
Rahnella aqatilis :https://www.atcc.org/Products/All/33071.aspx"
Bacillus badius : https://www.atcc.org/Products/All/15574-MINI-PACK.aspx
Bacillus licheniformis : https://www.atcc.org/Products/All/15580.aspx
How would your project be used in the real world?
-We want our project to be developed in nutrition field as using engineered bacteria as supplements. By introducing them into people's intestine in the future, we think that we can really contribute to the solution of protein shortage.
<Our Lab Work>
What is the Safety Level of your Lab ?
-We work in Level 1 Labs and we use only Risk Group 1 organisms.
What is the measure you have taken to reduce risk during the experiment?
*Do not eat, drink and smoke in laboratories.
*Wear lab coats inside the laboratories.
*Do not bring in things from the outside as much as possible.
*Open containers with bacteria and handle them inside the clean bench.
*Wash hands before and after experiment.
What safety training have you received?
-We have been taken safety trainings, such as lab access and rules by our PIs. We learned about biosafety levels, disinfection and sterilization, emergency procedures and transport rules in the genetic recombination experiment lecture at the university.