Team:CMUQ/Protocols
The following are the Protocols used:
-
CRISPR:
- Initial Test
- Positive Control
- Dsp double digest EcoR1 and pst1
- Gel Extraction
- PCR gBlocks
- Single Digest of Ligation Reaction
- Single Digest PSC1B3 with EcoR1
- Calibration 1
- Calibration 2
- Calibration 3
- Cell Calibration Day 1
- Cell Calibration Day 2
- Cell Calibration Day 3
- Cell Calibration Day 3: CFU to OD600
- Cell Measurement Summary
- Competent Cell Test
- 1% mini Agarose
- LB-Chl Plate prep
- Preparation of SD-PAGE gel
- SOC Media Preparation
- Double Digest with EcoR1 and Spe1
- IPTG Induction
- Ligation of pSB1C3 And Dsp
- Plasmid Extraction (mini prep)
- Re-precipitation of DNA
- Ultra-competent Cell Test (One shot DH5alpha calls)
Improvement Project:
Calibration
Interlab
Preparation
Improvement: