Team:Chalmers-Gothenburg/Protocols

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• LABWORK
  Binding Detection Treatment

  InterLab BioBricks Protocols Safety
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Protocols

Workflow Overview

In the figure below an overview of the cloning process, shared by all project parts, is presented.

Figure 1: In this picture a schematic overview of the cloning process is presented, from amplification oof genes and digestion pasmid to thransformation of plasmid into yeast.

Protocols for PCR

Thermo Scientific DreamTaq DNA Polymerase
Thermo Scientific Phusion High-Fidelity DNA Polymerase
PrimeSTAR HS DNA Polymerase
Fusion PCR

Protocols for purification

Thermo Scientific GeneJET PCR Purification Kit
Thermo Scientific GeneJET Gel Extraction Kit

Protocols for digestion and assembly

FastDigest Restriction Enzymes
Gibson Assembly Master Mix
DNA Insert Ligation into Vector DNA

Protocols for Saccharomyces cerevisiae

Transformation (Chemical)
Transformation (Quick version)
Isolation of genomic DNA

Protocols for Escherichia coli

Transformation (Heat shock)
Thermo Scientific GeneJET Plasmid Miniprep Kit

Protocols for Biobricks

BioBricks Assembly Protocol

Protocols for Interlab study

Interlab study

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CONTACT INFORMATION:

CHALMERS University of Technology, Gothenburg, Sweden, igemgothenburg@gmail.com

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