Team:Dalhousie Halifax NS/Procedures
Protocols
Using restriction enzymes to cleave framents of DNA. Often used in cloning and verifying the insertion.
Used to separate DNA on the basis of size. After Digestion, this lets us see how big our segements.
Used to join inserts into a vector. Requires cloning after to seal "nicks" in the back bone.
We use heat to introduce small pores in the bacterial membrane to allow our DNA constructs to enter the cell
