Team:Leiden/Parts
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Parts
We have isolated and characterized a collection of 27 promoters in E. coli, related to a variety of endogenous stress responses, ranging from DNA damage to misfolded protein response. These promoter BioBricks were designed to be
combined with fluorophore and chromoprotein reporters, in order to Read more about our visualisation system on our Project
Description page visualize bacterial cell stress. We have also submitted a selection of our promoters already coupled to fluorophore and chromoprotein reporters in the form of composite
parts. Additionally, we have submitted BioBricks with amplified stress GFP signals, to make stress detection even easier. As a collection, our promoters serve as an extensive stress screening system in E. coli, which can be used for
antibiotic screening. Additionally, the wide variety of promoters in our collection allows them to be used for many other purposes in future research and iGEM projects.
We have submitted the following parts to the parts registry:
All Parts
| Favorite part | Name | Type | Description | Designers | Length (bp) |
|---|---|---|---|---|---|
| BBa_K261000 | Basic | pAlkA | Marjolein Crooijmans | 300 | |
| BBa_K2610001 | Basic | pDegP | Marjolein Crooijmans | 190 | |
| BBa_K2610002 | Basic | pIbpA | Marjolein Crooijmans | 183 | |
| BBa_K2610003 | Basic | pIbpB | Marjolein Crooijmans | 282 | |
| BBa_K2610004 | Basic | pKatG | Marjolein Crooijmans | 143 | |
| BBa_K2610005 | Basic | pLamB | Marjolein Crooijmans | 300 | |
| BBa_K2610006 | Basic | pMalK | Marjolein Crooijmans | 298 | |
| BBa_K2610007 | Basic | pMicF | Marjolein Crooijmans | 301 | |
| BBa_K2610008 | Basic | pMreB | Marjolein Crooijmans | 300 | |
| BBa_K2610009 | Basic | pRecA | Marjolein Crooijmans | 300 | |
| BBa_K2610010 | Basic | pRecN | Marjolein Crooijmans | 300 | |
| BBa_K2610011 | Basic | pSecA | Marjolein Crooijmans | 300 | |
| BBa_K2610012 | Basic | pSulA | Marjolein Crooijmans | 300 | |
| BBa_K2610013 | Basic | pUmuC | Marjolein Crooijmans | 300 | |
| BBa_K2610014 | Basic | pUmuD | Marjolein Crooijmans | 300 | |
| BBa_K2610015 | Basic | pYmfM | Marjolein Crooijmans | 117 | |
| BBa_K2610016 | Basic | pYncC | Marjolein Crooijmans | 50 | |
| BBa_K2610017 | Composite | pMicF-GFP | Marjolein Crooijmans | 1027 | |
| BBa_K2610018 | Composite | pKatG-GFP | Marjolein Crooijmans | 869 | |
| BBa_K2610019 | Composite | pDnaK-GFP | Marjolein Crooijmans | 1186 | |
| BBa_K2610020 | Composite | pClpB-GFP | Marjolein Crooijmans | 1021 | |
| BBa_K2610021 | Basic | pRpoE | Daphne van den homberg | 298 | |
| BBa_K2610022 | Basic | pCpxR | Daphne van den homberg | 297 | |
| BBa_K2610023 | Basic | pBaeS | Daphne van den homberg, Mees Fox |
300 | |
| BBa_K2610024 | Basic | pSoxR | Daphne van den homberg | 300 | |
| BBa_K2610025 | Basic | pSpy | Daphne van den homberg | 291 | |
| BBa_K2610026 | Basic | pInaA | Daphne van den homberg, Mees Fox |
299 | |
| BBa_K2610027 | Basic | pBaeR | Daphne van den homberg, Mees Fox |
297 | |
| BBa_K2610028 | Composite | pSoxS-T7 RNA polymerase | Laurens ter Haar, Germaine Aalderink |
2955 | |
| BBa_K2610029 | Composite | pT7-GFP | Laurens ter Haar, Germaine Aalderink |
749 | |
| BBa_K2610030 | Basic | pSoxS | Marjolein Crooijmans | 297 | |
| BBa_K2610031 | Composite | pSoxS-GFP | Marjolein Crooijmans | 1023 | |
| BBa_K2610032 | Composite | pSoxS-mCherry | Maaike de Jong, Lotte Weel |
1017 | |
| BBa_K2610033 | Composite | pSoxS-AmilCP | Marjolein Crooijmans | 972 | |
| BBa_K2610034 | Composite | pSoxS-GFP-pSoxS-GFP | Maaike de Jong, Lotte Weel |
2054 | |
| BBa_K2610035 | Composite | pSoxS-GFP-GFP-pSoxS-GFP-GFP | Maaike de Jong, Lotte Weel |
3506 | |
| BBa_K2610036 | Basic | pCspA | Marjolein Crooijmans | 295 | |
| BBa_K2610037 | Composite | pCspA-GFP | Marjolein Crooijmans | 1021 | |
| BBa_K2610038 | Composite | pCspA-AmilCP | Marjolein Crooijmans | 970 | |
| BBa_K2610039 | Composite | pCspA-spisPink | Marjolein Crooijmans | 979 | |
| BBa_K2610040 | Basic | pGapA | Marjolein Crooijmans | 300 | |
| BBa_K2610041 | Composite | pGapA-AmilCP | Marjolein Crooijmans | 975 | |
| BBa_K2610042 | Composite | pGapA-spisPink | Marjolein Crooijmans | 1028 | |
| BBa_K2610043 | Composite | pSoxS-mCherry-pKatG-GFP | Maaike de Jong, Lotte Weel |
1894 | |
| BBa_K2610044 | Composite | pSoxS-mCherry-MicF-GFP | Maaike de Jong, Lotte Weel |
2052 |
Basic Parts
| Favorite part | Name | Type | Description | Designers | Length (bp) |
|---|---|---|---|---|---|
| BBa_K2610000.. | Basic | pAlkA | Marjolein Crooijmans | 300 | |
| BBa_K2610001 | Basic | pDegP | Marjolein Crooijmans | 190 | |
| BBa_K2610002 | Basic | pIbpA | Marjolein Crooijmans | 183 | |
| BBa_K2610003 | Basic | pIbpB | Marjolein Crooijmans | 282 | |
| BBa_K2610004 | Basic | pKatG | Marjolein Crooijmans | 143 | |
| BBa_K2610005 | Basic | pLamB | Marjolein Crooijmans | 300 | |
| BBa_K2610006 | Basic | pMalK | Marjolein Crooijmans | 298 | |
| BBa_K2610007 | Basic | pMicF | Marjolein Crooijmans | 301 | |
| BBa_K2610008 | Basic | pMreB | Marjolein Crooijmans | 300 | |
| BBa_K2610009 | Basic | pRecA | Marjolein Crooijmans | 300 | |
| BBa_K2610010 | Basic | pRecN | Marjolein Crooijmans | 300 | |
| BBa_K2610011 | Basic | pSecA | Marjolein Crooijmans | 300 | |
| BBa_K2610012 | Basic | pSulA | Marjolein Crooijmans | 300 | |
| BBa_K2610013 | Basic | pUmuC | Marjolein Crooijmans | 300 | |
| BBa_K2610014 | Basic | pUmuD | Marjolein Crooijmans | 300 | |
| BBa_K2610015 | Basic | pYmfM | Marjolein Crooijmans | 117 | |
| BBa_K2610016 | Basic | pYncC | Marjolein Crooijmans | 50 | |
| BBa_K2610021 | Basic | pRpoE | Daphne van den Homberg | 298 | |
| BBa_K2610022 | Basic | pCpxR | Daphne van den Homberg | 297 | |
| BBa_K2610023 | Basic | pBaeS | Daphne van den Homberg, Mees Fox | 300 | |
| BBa_K2610024 | Basic | pSoxR | Daphne van den Homberg | 300 | |
| BBa_K2610025 | Basic | pSpy | Daphne van den Homberg | 291 | |
| BBa_K2610026 | Basic | pInaA | Daphne van den Homberg, Mees Fox | 299 | |
| BBa_K2610027 | Basic | pBaeR | Daphne van den Homberg, Mees Fox | 297 | |
| BBa_K2610030 | Basic | pSoxS | Marjolein Crooijmans | 297 | |
| BBa_K2610036 | Basic | pCspA | Marjolein Crooijmans | 295 | |
| BBa_K2610040 | Basic | pGapA | Marjolein Crooijmans | 300 |
Composite Parts
| Favorite part | Name | Type | Description | Designers | Length (bp) |
|---|---|---|---|---|---|
| BBa_K2610017 | Composite | pMicF-GFP | Marjolein Crooijmans | 1027 | |
| BBa_K2610018 | Composite | pKatG-GFP | Marjolein Crooijmans | 869 | |
| BBa_K2610019 | Composite | pDnaK-GFP | Marjolein Crooijmans | 1186 | |
| BBa_K2610020 | Composite | pClpB-GFP | Marjolein Crooijmans | 1021 | |
| BBa_K2610028 | Composite | pSoxS-T7 RNA polymerase | Laurens ter Haar, Germaine Aalderink | 2955 | |
| BBa_K2610029 | Composite | pT7-GFP | Laurens ter Haar, Germaine Aalderink | 749 | |
| BBa_K2610031 | Composite | pSoxS-GFP | Marjolein Crooijmans | 1023 | |
| BBa_K2610032 | Composite | pSoxS-mCherry | Maaike de Jong, Lotte Weel | 1017 | |
| BBa_K2610033 | Composite | pSoxS-AmilCP | Marjolein Crooijmans | 972 | |
| BBa_K2610034 | Composite | pSoxS-GFP-pSoxS-GFP | Maaike de Jong, Lotte Weel | 2054 | |
| BBa_K2610035 | Composite | pSoxS-GFP-GFP-pSoxS-GFP-GFP | Maaike de Jong, Lotte Weel | 3506 | |
| BBa_K2610037 | Composite | pCspA-GFP | Marjolein Crooijmans | 1021 | |
| BBa_K2610038 | Composite | pCspA-AmilCP | Marjolein Crooijmans | 970 | |
| BBa_K2610039 | Composite | pCspA-spisPink | Marjolein Crooijmans | 979 | |
| BBa_K2610041 | Composite | pGapA-AmilCP | Marjolein Crooijmans | 975 | |
| BBa_K2610042 | Composite | pGapA-spisPink | Marjolein Crooijmans | 1028 | |
| BBa_K2610043 | Composite | pSoxS-mCherry-pKatG-GFP | Maaike de Jong, Lotte Weel | 1894 | |
| BBa_K2610044 | Composite | pSoxS-mCherry-MicF-GFP | Maaike de Jong, Lotte Weel | 2052 |
Primers
E. coli primers
| primer | Sequence (5'- 3') |
|---|---|
| abgT-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAGTTGGAAACTGCCTGTTGC |
| abgT-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTATCCTCGGTTGTTGTGTTTGC |
| acpP-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCAACGCGGTTGCATTCCTG |
| acpP-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTAACTCTTAAATTTCCTATCAA |
| alkA-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGACATCATGATGGCGGTACAG |
| alkA-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTACGCATCTCCTTATTCATCCG |
| baeR-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCACTGACAGCGGCGGCAGCC |
| baeR-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTAACTTCTCTCTGTAAATCCCG |
| baeS-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCTCCTCCATGAACACCCTGAC |
| baeS-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTATGCGCGCTCCTTTTTCGCCG |
| clpB-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGTATCTGGCGGATATTTATC |
| clpB-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTAAACTCCTCCCATAACGGATC |
| cpxR-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCTTATGCCGTCGAACATATG |
| cpxR-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTATGTTTAAATACCTCCGAGGC |
| cspA-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCAGTTGATGGAATAGACTTTTATC |
| cspA-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTAAGTGTATTACCTTTAATAATTAA |
| cspG-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGGGACAAACAAATTCCTTAC |
| cspG-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTAATTTTACTTCCTTCATTTTG |
| cutF-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCAGCCTGCCAGAGTATTAC |
| cutF-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTACCATTCCTTCTTTTTATTCC |
| cysH-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAGCGCCGGGTCGCTACAAC |
| cysH-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTAAGATTCCTCACTGTTTGCC |
| degP-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCGGTATGACCGACCTCTATG |
| degP-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTAGTATTTCAGTCTCGATTAAC |
| dnaK-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCGTAATCTTTTCTTTTTATTAC |
| dnaK-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTACTAAACGTCTCCACTATATATTC |
| emrA-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGGTCACGAGTTTTTGCGCG |
| emrA-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTAATTGTTCTCCACGATCTTC |
| folC-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCTGATCGAGAAAGGCGCGATC |
| folC-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTAGGTATCCGCTGATTCGTTACG |
| gapA-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGATTCTAACAAAACATTAAC |
| gapA-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTAATATTCCACCAGCTATTTG |
| htpG-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAAGTGATCCTCGCCACCAAC |
| htpG-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTAGTAGGTCTACCTCAATAATG |
| htpX-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGATTACCAGGAGCCGGATC |
| htpX-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTAAATTTTCTTTTTACCTCAG |
| ibpA-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCTCAGTCTATGCAATAGAC |
| ibpA-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTAAATCAATAGCTCCTGAAATC |
| ibpB-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGAGCGCACCTATCTGTACCAG |
| ibpB-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTAAGTCATTTCTCCTTCTAAG |
| iclR-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCGATAACTCTG |
| iclR-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTAGACAGTCTCTT |
| inaA-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGAACGGAACACAATTCAC |
| inaA-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTAACCCTATCGCCTGTCATT |
| katG-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGCTTAATTAAGATCAATTTG |
| katG-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTACAATGTGCTCCCCTCTACAG |
| lamB-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTGACGTCATCCTTGAGGGTG |
| lamB-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTATCTATCTCCTGAGTCATTGC |
| lysU-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGGCCTTTGCTACCCGTTTTC |
| lysU-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTTGGTTCCTCTAAATCCAG |
| malK-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTAGCCCTTCTCCCTTTGTAAC |
| malK-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGCAACCTCTTTCCATCCTC |
| marA-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCTGCGCGGCGTGTATTACTC |
| marA-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTAACCTCTTTTTTGTTTACGGC |
| marR-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCAGGCCAATTGCTTTAAAC |
| marR-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTAATGGATTAAGCGACCCAATG |
| micF-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGCTACCAGCAGAGCTGGGACC |
| micF-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTAACCTTGAATTATTATTGCTTG |
| mreB-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCGTTTAATCCGTTTTC |
| mreB-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTAACTAAGGGATAATCCTG |
| nusA-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGTGCTGGATGTTGAAGATCC |
| nusA-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTACGCGGGCTTTTCACCTCATC |
| pspA-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCAGCTCTTTACCGGTGCCGC |
| pspA-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTAAATGTTGTCCTCTTGATTTC |
| pspE-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGAATACTCGCTGGCAACAGGC |
| pspE-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTAGTAAGCGTCCTTCTATGGATG |
| pspF-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGTACTTCAACCAGTGTATCTTC |
| pspF-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTAGATGAAATTCGCCACTTGTTAG |
| rcsA-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGAAATATTTGTTTTAAGCTC |
| rcsA-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTAGGCATACCCTCACTCAATGC |
| recA-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCGGCTCGTGCTGATTATGCC |
| recA-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTTTACTCCTGTCATGCCGG |
| recN-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTCATAAACAGCAGCAGC |
| recN-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTAAGTCGTTTTCCTGTATG |
| relA-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCGATAAAGTGTTGC |
| relA-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTACGTCCTCTCCTTTAGGGACC |
| rpoE-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGGCGGCGAGAAAAAGAGAAGTTAC |
| rpoE-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTACCGAGGTAAAGTCTCCCCAAAC |
| rpoH-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGGTGGCAGCGTGGCTTGCCACG |
| rpoH-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTATCAAATCCTCTCAATCGATATC |
| rseA-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTGAAAACTTCGAAAGTGGC |
| rseA-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTAGCCTAATACCCTTATCCAG |
| secA-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGATCTTTCTTTCGCAATGG |
| secA-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTAAATAAAATCTCAAACGCCC |
| secE-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCTGCGTTTCGCAATCCGTGAAGG |
| secE-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTAAAACCAACCTGTCAGAAGTTTTC |
| secG-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGGTGGAAGGCGAAGACGAAG |
| secG-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTAAATCAACCTGTTCCTTGCGG |
| secY-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGCGGTTTCGAGGGTGGTCAG |
| secY-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTACTGCTACTTATTCCTCGATT |
| soxR-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGGACGCTCGGTGGTGCGC |
| soxR-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTAAAATCGCTTTACCTCAAG |
| soxS-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGCCAGCGGAATGCCAATACG |
| soxS-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTAAAATCTGCCTCTTTTCAGTG |
| spY-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCTTTCTTATAAATTAATACAG |
| spY-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTAATTCTATATCCTTCCTTTCAG |
| sulA-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGACAATGATCGGTATTCAATTG |
| sulA-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTAAATCAATCCAGCCCCTGTGAG |
| T7-P1 | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTAATACGACTCACTATAGGGTACTAGTAGCGGCCGCTGCAGGAAGAAAC |
| T7-P2 | GTTTCTTCCTGCAGCGGCCGCTACTAGTACCCTATAGTGAGTCGTATTACTCTAGAAGCGGCCGCGAATTCGAAGAAAC |
| tdcA-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTTGCCTGGTAATTATCCGG |
| tdcA-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTAAACGACCTACGTTAATTAC |
| umuC-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGAATCAACTGTTGATCCAGC |
| umuC-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTACAGCGCATCGCCTTAACGAC |
| umuD-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGATCTAATTCATAGTTAGCCGGG |
| umuD-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTAAATAATCTGCCTGAAGTTATAC |
| uvrA-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGATTACCAACGAGAATAACC |
| uvrA-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTATCACCTTTCCCGGATTAAAC |
| yedU-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCGGGATATAAATATTCATC |
| yedU-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTAAGTGTATTCCTTATTGTTGC |
| ygfZ-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCGCGCATACCACGGCGGCATGCC |
| ygfZ-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTAAATAGAGTCCTGAGTTGCTTAAC |
| ymfM-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGGAGCATGCAGCACTTGTC |
| ymfM-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTAGCGTTAGTTTCTCCACAAC |
| yncC-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCTTCTGCCATGCAGGC |
| yncC-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTAATTCCTTCGCGTG |
B. subtilis primers
| primer | Sequence (5'- 3') |
|---|---|
| fabHB-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGAACGCCGCCTTCCAGCAGC |
| fabHB-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTAATGAATCACTCCTTATGGTC |
| liaI-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCGGCTCAGGTAAAGTGATCGC |
| liaI-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTAGCAGATCCTCCTTTCGTTTTC |
| yhel-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGATATATTAGAAGGCGATCC |
| yhel-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTAAATAACACCCCATATTTATC |
| yorB-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCAGAGATTCAGATCAAGGC |
| yorB-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTAACTCTCATCCCCTGTTTTG |
| ypuA-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTCAATTTTGTTCCCGTCTTC |
| ypuA-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTAAGCAGTTTGACCTCCTTC |
| yvgS-P-Prefix | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCGTTGTTTGCCGGGTTTGTTCC |
| yvgS-P-Suffix | GTTTCTTCCTGCAGCGGCCGCTACTAGTAGAACGGCACCTCCAGAAAATAG |