Team:Munich/deletioncasette2.html
Creating a Selection Cassette from pSB1C3
2018/08/28| Participants: | Dominic Schwarz |
| Protocol: | PCR, Agarose gel, Gel extraction |
| Notes: | Knockouts of both msb-B, RecBCD Primer with 50bp homology regions, Ta: 62°C, Elongation: 45s Expected: 1,1kb |
| Results: | 2 PCR reactions worked. One reaction for a resistance cassette flanked with FRT sites didnt work. Elena (Advisor) suggested that the template might be incorrect. |
