Team:Munich/purify2.html
T7 and T4 DNA Purification with Phage DNA Isolation Kit
2018/07/25- 2018/08/02| Participants: | Brigit Tunaj |
| Protocol: | DNA Cleanup Kit, Phage T7 DNA purification |
| Notes: | DNAse I treatment and Proteinase K treatment |
| Results: | Clear bands, without E. coli contaminations. Next step: Nanopore sequencing T7 Phage stock: 9*10^9 PFU/mL; T4 Phage stock: 1,5*10^10 PFU/mL |
T4 and T7 Stock and DNA Purification with Phage DNA Isolation Kit
2018/08/07- 2018/08/08| Participants: | Sophie Kurzbach |
| Protocol: | DNA Cleanup Kit, Phage T7 DNA purification |
| Notes: | No notes |
| Results: | T7 Phage stock: 2,2*10^10 PFU/mL T4 Phage stock: 6*10^5 PFU/mL |
3S and P3 Phage Stock Solution and DNA Preparation
2018/08/07- 2018/08/08| Participants: | Sophie Kurzbach |
| Protocol: | DNA Cleanup Kit, Phage T7 DNA purification |
| Notes: | No notes |
| Results: | P3 Phage Stock: 1,8*10^9 PFU/mL; DNA: 10 ng/µL 3S Phage Stock: 3,5*10^8 PFU/mL; DNA: 22ng/µL |
Succrose Gradient for Ultra Centrifugation of Phage Solution
2018/05/17- 2018/05/18| Participants: | Sophie Kurzbach, Kilian Vogele |
| Protocol: | Ultracentrifugation |
| Notes: | No notes |
| Results: | Did not work out as planned |
T4 Phage Solution Preparation
2018/05/16- 2018/05/18| Participants: | Sophie Kurzbach |
| Protocol: | Phage T7 DNA purification |
| Notes: | Steps 1-7 |
| Results: | Did not work out as planned |
T4 and T7 DNA Purification with Phage DNA Isolation Kit
2018/08/15- 2018/08/16| Participants: | Brigit Tunaj |
| Protocol: | DNA Cleanup Kit, Phage T7 DNA purification |
| Notes: | DNAse I treatment and Proteinase K treatment |
| Results: | Clear bands, without E. coli contaminations, needed for phage assembly |
T7, T4 and 3S Stock Preparation and Cleanup
2018/08/20| Participants: | Brigit Tunaj |
| Protocol: | DNA Cleanup Kit, Phage T7 DNA purification |
| Notes: | DNAse treatment |
| Results: | Good enough for DNA Assembly but not for Sequencing. Therefore new cleaning up methods have been tried out by talking with Quirin and Kilian |
T4 Stock Preparation and Cleanup
2018/08/21- 2018/8/24| Participants: | Sophie Kurzbach |
| Protocol: | DNA Cleanup Kit, Phage T7 DNA purification |
| Notes: | No notes |
| Results: | T4 Phage stock: 7,7*10^10 PFU/mL, DNA: 1,1 nM and 14,9 nM |