Team:Northwestern/InterLab

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Team MetaSense participated in the Fifth International InterLaboratory Measurement Study in synthetic biology. The InterLab experiment aims to improve the measurement tools available to both the iGEM community and the synthetic biology community as a whole by comparing GFP fluorescence in different labs using the same protocol, as established by iGEM headquarters. Our results were formally submitted and are summarized in our notebook below.


InterLab Notebook

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Results and Conclusions

As anticipated, our fluorescein standard curve was approximately linear (Figure 1). Any non-linearity most likely indicates errors in pipetting, which will be accounted for by the iGEM measurement committee.

Calibrations



Figure 1 - Particle standard curve



Figure 2 - Fluorescein standard curve




Results


Figure 3 - 36 Transformed colonies used for CFU calculations