Team:UIOWA/Attributions
Attributions
Brendan Owens & Integrated DNA Technologies:
We want to thank IDT for providing us with primers and M-blocks for our regulators & promoters. We want to give a special shoutout to Brendan Owens who took us on a personal tour of IDT and their synthetic biology facility in Coralville, IA.
Opentrons:
We want to thank Opentrons for awarding our team with the OT2 pipetting robot and providing general support for installation and implementation. We cleverly named our bot, "Opentrons Prime"!
Boas Pucker:
We want to thank our iGEM mentor, Boas Pucker. He has been a huge help in aiding us with troubleshooting, staying on top of deadlines and general support.
Jacob Beal:
We would like to thank Dr. Jacob Beal. Dr. Beal spoke with our team about standardization in measurement and provided some tips on how to manage communication among multiple labs as an iGEM team.
Shahaed Hasan
We would like to thank Shahead Hasan for being our student mentor. Shahead provided insight into the 2017 iGEM project and helped us dig into some of the scientific papers relevant to our project.
Mason Lamarche
We would like to thank former 2017 UIOWA iGEM member, Mason, for interviewing our group for a 23andMe article.
Nicholas (Niko) McCarty
We would like to thank the founder of the UIOWA iGEM team. Niko met with us, brought us coffee and bagels and provided some scientific background and insight into current news and advances in synthetic biology.
Sander's lab
Mentor: Dr. Ed Sander
Lab advisor: Dr. Sathivel Chinnathambi
Students: Sean Ryan, Sandra Castillo, Jennifer Farrell
Contributions:
- Interlab
- Device testing
- Hanko device transformation (Dr. Sander)
Ellermeier's lab
Mentor: Dr. Craig Ellermeier
Students: Brynn Kyleakin Helm, Kathrine Amick
Contributions:
- Transformed biobricks into B. subtilis
- Transformed biobricks into E. coli
Fuentes's lab
Mentor: Dr. Ernesto Fuentes
Lab advisor: Dr. Young Joo Sun
Students: Joe Davis, Jianwei Hu
Contributions:
- Characterized LysR transcription regulator protein.
Fassler's lab
Mentor: Dr. Jan Fassler
Students: Delaney Soule, Yishuo Jang, Eleanor Bessner, Shao Yang Zhang, Jennifer Farrell, Rafael Linares.
Contributions:
- Characterization of parts
- Transformation and characterization of 2017 parts
- Basic lab support
Goldrush: University of Iowa’s student fundraising platform
- Theresa Jubert: University of Iowa GoldRush specialist
- Project Manager: Delaney Soule
- Assistants to Manager: Joe Davis, Rafael Linares
- Fundraisers: Katherine Amick, Eleanor Bessner, Sandra Castillo, Joe Davis, Jennifer Farrell, Yishuo Jiang, Jianwei Hu, Kyleakin Brynn Helm, Sean Ryan, Delaney Soule, Shao Yang Zhang
- Significant Contributors: Sue Bessner, Charles Brenner, William Davis, Matthew & April Helm, Edward Helm, Jeff & Jacqueline Martini, Janet McGurk, Roxie & Dick Merkel, Steve Ryan,Ted & Beverly Sanders,Patrick Sheehy, Judy & Kevin Soule, Wei Tao, Manlu-tababiaoji Xie, Shao Yang Zhang
Iowa State Fair STEM booth education, community outreach and activities related to synthetic biology
- Fair Booth Hosts: Katherine Amick, Rafael Linares, Rafael Linares, Yishuo Jiang, Joe Davis
- Poster and Pamphlet: Shao Yang Zhang
- Activity: Jennifer Farrell
iGEM Midwest Jamboree MSU: Midwest US University meet-up
- Attendees: Katherine Amick, Brynn Kyleakin Helm, Jianwei Hu, Delaney Soule
West Liberty High School: Synthetic Biology Clinic Mosquito Escape room
- Activity Organizers: Katherine Amick, Eleanor Bessner, Jennifer Farrell, Delaney Soule
University of Iowa: Honors Primetime New Student Lab Orientation
- TA’s: Katherine Amick, Brynn Kyleakin Helm, Sean Ryan
University of Iowa: Synthetic Biology lab course
- Mentors: Dr. Jan Fassler, Dr. Craig Ellermeier, Dr. Ed Sander
- Teacher's Assistant: Tanner Reeb
- We would like to thank our teaching assistant for the Synthetic Biology class. He ensured all the reagents for class were prepared and available. He also attended meetings and provided some insight and assistance with data analysis and interpretation.
- Students: all
- The course objective aims to establish standardized lab practices for the 2018 iGEM team using the iGEM protocols and invited interested University students to participate in an abridged iGEM experience. The course exposure included recreating the 2017 interlab and working with actual iGEM Biobricks to demonstrate applied principles of synthetic biology.
The Voayers of Plasmo, Molecular Defender-
Animated story to bridge the gap between STEM student outreach and fans of sci-fi/COMICON. We wanted to bridge the gap between outreach attempts to students who self-identify as STEM tracked and fans of sci-fi/COMICON aka: future world citizens who would be favorably disposed and have a potential aptitude for STEM concepts.
- Story by Brynn Kyleakin Helm, Drawings by Katherine Amick
Jorge Moreno
We would like to thank our student mentor, Jorge Moreno. Jorge went above and beyond attending meetings and mentoring our Outreach and Human Practices group. Good luck at Princeton!
Meeting with Dr. Anya Prince
- Dr. Prince currently serves as University of Iowa professor and attorney who specializes in the ethics of genetics and biotechnology. We would like to thank her for taking the time to sit down and discuss with us possible issues with working in synthetic biology.
LanzaTech, Skokie, IL
- Michael Koepke (business)– Team Leader synthetic Biology
- Mentor: Dr. Ed Sander
- Students: Jennifer Farrell, Sean Ryan, Delaney Soule
- Industrial applications in synthetic biology and biotechnology
- Applications and industrial expectations for our biosensor
Zhou, Shenfang, Ainala, Satish Kumar, Seol, Eunhee, Nguyen, Trinh Thi, Park, Sunghoon 2015 Inducible gene expression system by 3-hydroxypropionic acid Biotechnology for Biofuels 8:169
Kalantari, Aida, Chen, Tao, Ji, Boyang, Stancik, Ivan, Ravikumar, Vaishnavi, Franjevic, Damjan, Saulou-Berion, Claire, Goelzer, Mijakovic, Ivan 18April 2017 Conversion of glycerol to 3-hydroxypropanoic acid by genetically engineered Bacillus subtilis Frontiers in Microbiology
Hanko, Erik K, Minton, Nigel P, Malys, Naglis, 11May 2017 Characterisation of a 3-hydroxypropionic acid- inducible system from Pseudomonas putida for orthogonal gene expression control in Escheria coli and Cupriavidus necator Nature Scientific Reports p>
Beal, Jacob, Haddock-Angelli, Traci, Gershater, Markus, de Mora, Kim, Lizarazo, Meagan, Hollenhorst, Jim, Rettberg, Randy, iGEM Interlab Study Contributors 3March 2016 Reproducibility of Fluorescent Epression from Engineered Biological Constructs in E. coli Plos One
Buckley, Anthony M, Jukes, Caitlin, Douce, Gillian 21 March 2016 Lighting up Clostridium difficile reporting gene expression using fluorescent lov domains Nature Scientific Reports 23463