Team:USP-Brazil/Part Collection

Wiki - iGEM Brazil

The Quorum Sensing Collection

Our collection of Quorum Sensing parts may very well be considered the apex of our 2018 project. As we set on a goal to improve the repertoire of available Quorum Sensing parts and to improve their characterization, we arrived at an extensive array of basic and composite parts. Many parts already existed on the Registry, with varying amounts of characterization information, but we decided to include them in our Quorum Sensing Collection as a way to centralize this information for future reference. We hope that this collection may be the cornerstone for innovative synthetic biology projects, which use quorum sensing (and bacterial communication as a whole) as circuit building tool.

Basic Parts - Promoters

Part Name Part Number Length
pLux BBa_R0062 55 bp
Modified pLux BBa_K2771000 55 bp
pLas BBa_K091117 126 bp
Modified pLas BBa_K2771001 55 bp
pRhl BBa_R0071 53 bp
Modified pRhl BBa_I14017 51 bp
pTra BBa_K553002 151 bp
Modified pTra BBa_K2771002 54 bp
pRpa BBa_K1421005 86 bp
Modified pRpa BBa_K2771003 56 bp
pCin BBa_R0078 225 bp

Basic Parts - Synthases

Part Name Part Number Length
LuxI BBa_C0061 643 bp
LasI BBa_C0178 609 bp
LasI (L72F) BBa_K2771009 603 bp
RhlI BBa_C0170 609 bp
CinI BBa_C0076 727 bp
RpaI BBa_K1421006 657 bp
TraI BBa_K553001 639 bp
TraI (codon optimized) BBa_K2771004 639 bp
TraI (K34G) BBa_K2505033 639 bp
AubI BBa_K2033000 714 bp
BjaI BBa_K2033002 672 bp
BraI BBa_K2033004 684 bp
CerI BBa_K2033006 634 bp
SinI BBa_K2033008 645 bp
EsaI Device BBa_K1670004 673 bp

Basic Parts - Receivers

Part Name Part Number Length
LuxR BBa_C0062 781 bp
LuxR alternative seq. BBa_K2771008 750 bp
LasR BBa_C0179 723 bp
RhlR BBa_C0170 729 bp
CinR BBa_C0077 787 bp
RpaR BBa_K2771006 732 bp
TraR BBa_K2771007 702 bp
TraR (E192W) BBa_K2771005 708 bp

Composite Parts

The Registry’s range of quorum sensing-related composite part is simply massive. So we have selected a few that peaked our interest over the course of our project, and that may come in hand for future projects.

Part Name Part Number Length
pLux Ratiometric Activity Reporter BBa_K2771021 1928 bp
pLas Ratiometric Activity Reporter BBa_K2771022 1928 bp
pRhl Ratiometric Activity Reporter BBa_K2771023 1926 bp
pCin Ratiometric Activity Reporter BBa_K2771024 2098 bp
pTra Ratiometric Activity Reporter BBa_K2771025 1927 bp
pRpa Ratiometric Activity Reporter BBa_K2771026 1929 bp
Lux Sender Device (pBad) BBa_I13015 2016 bp
Las Sender Device (tetR) BBa_K574004 797 bp
Rhl Sender Device (pBad) BBa_K2771043 1982 bp
Cin Sender Device (pBad) BBa_K2771042 2100 bp
Rpa Sender Device (pBad) BBa_K2771045 2030 bp
Tra Sender Device (pBad) BBa_K2771044 2012 bp
Lux Receiver Device (tetR) BBa_F2620 1061 bp
Las Receiver Device (constitutive) BBa_K574009 1147 bp
Rhl Receiver Device (tetR) BBa_F2710 1007 bp
Cin Receiver Device (tetR) BBa_F2770 1185 bp
Rpa Receiver Device (constitutive) BBa_K2771055 930 bp
Tra Receiver Device (constitutive) BBa_K2771054 900 bp