The Quorum Sensing Collection
Our collection of Quorum Sensing parts may very well be considered the apex of our 2018 project. As we set on a goal to improve the repertoire of available Quorum Sensing parts and to improve their characterization, we arrived at an extensive array of basic and composite parts. Many parts already existed on the Registry, with varying amounts of characterization information, but we decided to include them in our Quorum Sensing Collection as a way to centralize this information for future reference. We hope that this collection may be the cornerstone for innovative synthetic biology projects, which use quorum sensing (and bacterial communication as a whole) as circuit building tool.
Basic Parts - Promoters
| Part Name | Part Number | Length |
|---|---|---|
| pLux | BBa_R0062 | 55 bp |
| Modified pLux | BBa_K2771000 | 55 bp |
| pLas | BBa_K091117 | 126 bp |
| Modified pLas | BBa_K2771001 | 55 bp |
| pRhl | BBa_R0071 | 53 bp |
| Modified pRhl | BBa_I14017 | 51 bp |
| pTra | BBa_K553002 | 151 bp |
| Modified pTra | BBa_K2771002 | 54 bp |
| pRpa | BBa_K1421005 | 86 bp |
| Modified pRpa | BBa_K2771003 | 56 bp |
| pCin | BBa_R0078 | 225 bp |
Basic Parts - Synthases
| Part Name | Part Number | Length |
|---|---|---|
| LuxI | BBa_C0061 | 643 bp |
| LasI | BBa_C0178 | 609 bp |
| LasI (L72F) | BBa_K2771009 | 603 bp |
| RhlI | BBa_C0170 | 609 bp |
| CinI | BBa_C0076 | 727 bp |
| RpaI | BBa_K1421006 | 657 bp |
| TraI | BBa_K553001 | 639 bp |
| TraI (codon optimized) | BBa_K2771004 | 639 bp |
| TraI (K34G) | BBa_K2505033 | 639 bp |
| AubI | BBa_K2033000 | 714 bp |
| BjaI | BBa_K2033002 | 672 bp |
| BraI | BBa_K2033004 | 684 bp |
| CerI | BBa_K2033006 | 634 bp |
| SinI | BBa_K2033008 | 645 bp |
| EsaI Device | BBa_K1670004 | 673 bp |
Basic Parts - Receivers
| Part Name | Part Number | Length |
|---|---|---|
| LuxR | BBa_C0062 | 781 bp |
| LuxR alternative seq. | BBa_K2771008 | 750 bp |
| LasR | BBa_C0179 | 723 bp |
| RhlR | BBa_C0170 | 729 bp |
| CinR | BBa_C0077 | 787 bp |
| RpaR | BBa_K2771006 | 732 bp |
| TraR | BBa_K2771007 | 702 bp |
| TraR (E192W) | BBa_K2771005 | 708 bp |
Composite Parts
The Registry’s range of quorum sensing-related composite part is simply massive. So we have selected a few that peaked our interest over the course of our project, and that may come in hand for future projects.
| Part Name | Part Number | Length |
|---|---|---|
| pLux Ratiometric Activity Reporter | BBa_K2771021 | 1928 bp |
| pLas Ratiometric Activity Reporter | BBa_K2771022 | 1928 bp |
| pRhl Ratiometric Activity Reporter | BBa_K2771023 | 1926 bp |
| pCin Ratiometric Activity Reporter | BBa_K2771024 | 2098 bp |
| pTra Ratiometric Activity Reporter | BBa_K2771025 | 1927 bp |
| pRpa Ratiometric Activity Reporter | BBa_K2771026 | 1929 bp |
| Lux Sender Device (pBad) | BBa_I13015 | 2016 bp |
| Las Sender Device (tetR) | BBa_K574004 | 797 bp |
| Rhl Sender Device (pBad) | BBa_K2771043 | 1982 bp |
| Cin Sender Device (pBad) | BBa_K2771042 | 2100 bp |
| Rpa Sender Device (pBad) | BBa_K2771045 | 2030 bp |
| Tra Sender Device (pBad) | BBa_K2771044 | 2012 bp |
| Lux Receiver Device (tetR) | BBa_F2620 | 1061 bp |
| Las Receiver Device (constitutive) | BBa_K574009 | 1147 bp |
| Rhl Receiver Device (tetR) | BBa_F2710 | 1007 bp |
| Cin Receiver Device (tetR) | BBa_F2770 | 1185 bp |
| Rpa Receiver Device (constitutive) | BBa_K2771055 | 930 bp |
| Tra Receiver Device (constitutive) | BBa_K2771054 | 900 bp |