Team:Uppsala/test-3

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Bigass heading

Exampel rubrik utan annat

Well, the way they make shows is, they make one show. That show's called a pilot. Then they show that show to the people who make shows, and on the strength of that one show they decide if they're going to make more shows. Some pilots get picked and become television programs. Some don't, become nothing. She starred in one of the ones that became nothing.

Random heading

Well, the way they make shows is, they make one show. That show's called a pilot. Then they show that show to the people who make shows, and on the strength of that one show they decide if they're going to make more shows. Some pilots get picked and become television programs. Some don't, become nothing. She starred in one of the ones that became nothing.

Exempel på rubrik 1

Well, the way they make shows is, they make one show. That show's called a pilot. Then they show that show to the people who make shows, and on the strength of that one show they decide if they're going to make more shows. Some pilots get picked and become television programs. Some don't, become nothing. She starred in one of the ones that became nothing

Bigass heading

Can remove heading if this will bein the middle of the page.

My money's in that office, right? If she start giving me some bullshit about it ain't there, and we got to go someplace else and get it, I'm gonna shoot you in the head then and there. Then I'm gonna shoot that bitch in the kneecaps, find out where my goddamn money is. She gonna tell me too. Hey, look at me when I'm talking to you, motherfucker. You listen: we go in there, and that Winston or anybody else is in there, you the first motherfucker to get shot. You understand?

My money's in that office, right? If she start giving me some bullshit about it ain't there, and we got to go someplace else and get it, I'm gonna shoot you in the head then and there. Then I'm gonna shoot that bitch in the kneecaps, find out where my goddamn money is. She gonna tell me too. Hey, look at me when I'm talking to you, motherfucker. You listen: we go in there, and that Winston or anybody else is in there, you the first motherfucker to get shot. You understand?

Look, just because I don't be givin' no man a foot massage don't make it right for Marsellus to throw Antwone into a glass motherfuckin' house, fuckin' up the way the talks. Motherfucker do that shit to me, he better paralyze my ass, 'cause I'll kill the motherfucker, know what I'm sayin'?

Bigass heading

Random heading

Well, the way they make shows is, they make one show. That show's called a pilot. Then they show that show to the people

Random Heading

Well, the way they make shows is, they make one show. That show's called a pilot. Then they show that show to the people who make shows, and on the strength of that one show they decide if they're going to make more shows. Some pilots get picked and become television programs. Some don't, become nothing. She starred in one of the ones that became nothing.

I'm serious as a heart attack

The path of the righteous man is beset on all sides by the iniquities of the selfish and the tyranny of evil men. Blessed is he who, in the name of charity and good will, shepherds the weak through the valley of darkness, for he is truly his brother's keeper and the finder of lost children. And I will strike down upon thee with great vengeance and furious anger those who would attempt to poison and destroy My brothers. And you will know My name is the Lord when I lay My vengeance upon thee.

RUBRIK NR 2

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Figure 2: Bilirubin test before/after affinity chromatography. Going from right to left the samples are:

• Symptoms

  Symptoms

  The release of larvae from cysts can lead to lesions, diarrhea, and potential weight loss. This condition is called cyathostominosis [1]. When untreated, the death tolI can reach up to 50%. During the seasonal rupture of cysts, millions of larvae can be released at the same time, which can result in severe and life-threatening consequences [5]. The infection of small strongyles is not one of presence, but one of quantity. They are not dangerous in small amounts and therefore it is difficult to tell whether a horse needs to be treated or not [9].
  
  If farmers had the possibility to know when to treat their horses, prevention of mass rupture and other severe consequences like increased resistance development could be achieved. To reach this goal we have developed a model that (based on multiple parameters) calculates the optimal amount of treatments in a specified period. This will avoid unnecessary use of anthelmintics by raising the awareness in farmers regarding when they actually need to treat their horses.

• Current Methods

  Current Methods of Detection

  Currently the only method for detecting/counting how many worms there are in an animal is counting nematode eggs in fecal samples. This method is not reliable and also requires farmers and ranchers to send in fecal samples to a lab with trained personnel [4]. This technique is expensive, inconsistent, and requires time sensitive shipping of samples. Currently many horse owners are reluctant to conduct the testing; treating their horses regardless of need which contributes to the resistance problem [10].

• Resistance Development

  Resistance Development

  Unfortunately, the extensive overuse of deworming drugs has now lead to the detection of worms that are resistant to the most commonly used drugs [2,4]. Since no new deworming drugs have been approved for use in horses the whole equine industry relies on macrocyclic lactones, currently the most common type of deworming drugs used. Unfortunately, cases of resistance among nematode adults have been spotted for macrocyclic lactones as well. So far, four studies with similar results spanning from Europe to North America have been published with concrete data, linking certain small strongyle species to reduced time until detection of eggs after deworming treatment, showing an increase in resistance[2].
  
  Moxidectin is a very common drug used. It is both hazardous for the environment and is also losing its effectiveness towards worm populations [14,15].
  
  Due to all of these facts, we have during our iGEM project developed a reporter that is suitable for the in vivo environment of horse intestines. Moreover, we have developed new applications on existing techniques to be able to find the promoter that would be coupled to the reporter which would at last create the worm buster.
  
  By implementing the model described under Symptoms together with the worm buster, horse owners will not only know how large the treatments should be but also when the treatments should happen. Thus they complement each other to minimize the amount of anthelmintics used; thereby helping to prevent resistance development.