Team:ZJU-China/AllParts

<!DOCTYPE html> Electrode

ALL PARTS  
OVERVIEW  

This year, we designed 23 parts, and all of them had been submitted to the iGEM Registry following the BioBricks assembly standard as described by RFC10.


Here is a brief description of our parts. If you want to get more detailed information about our parts, we strongly recommend you visit the corresponding parts pages on the iGEM BioBricks Registry.



Parts Table

Name

Type

Description

Length(bp)

Designer

BBa_K2721000

promoters

Modified T7 promoter 1

23

Huining Liang

BBa_K2721001

promoters

Modified T7 promoter 2

23

Huining Liang

BBa_K2721002

promoters

Modified T7 promoter 3

23

Huining Liang

BBa_K2721003

promoters

Modified T7 promoter 4

23

Huining Liang

BBa_K2721000

coding

Gene of csgA

525

Xinhui Li

BBa_K2721001

composite part

modified csgA expression system

893

Xinhui Li

BBa_K2721020

coding

Spycatcher with histag

306

Yunqian Li

BBa_K2721021

coding

Snoopcatcher with histag

366

Yunqian Li

BBa_K2721022

coding

Spycatcher with histag

369

Yunqian Li

BBa_K2721023

coding

SpyTag with GFP

759

Yunqian Li

BBa_K2721024

coding

RFP with SdyTag and SpyCatcher

1047

Yunqian Li

BBa_K2721025

coding

GFP with SnoopTag and SdyCatcher

1131

Yunqian Li

BBa_K2721026

coding

Snoopcatcher with YFP

1092

Yunqian Li

BBa_K2721027

coding

LDH with SdyTag and SpyCatcher

1116

Yunqian Li

BBa_K2721028

coding

Glucose Oxidase(GOx) with SnoopCatcher

2157

Yunqian Li

BBa_K2721029

coding

HRP with SnoopTag and SdyCatcher

1374

Yunqian Li

BBa_K2721030

composite part

SpyTag with GFP

1124

Yunqian Li

BBa_K2721031

composite part

RFP with SdyTag and SpyCatcher

943

Yunqian Li

BBa_K2721032

composite part

GFP with SnoopTag and SdyCatcher

1315

Yunqian Li

BBa_K2721033

composite part

Snoopcatcher with YFP

1274

Yunqian Li

BBa_K2721034

composite part

LDH with SdyTag and SpyCatcher

1300

Yunqian Li

BBa_K2721035

composite part

Glucose Oxidase(GOx) with SnoopCatcher

2341

Yunqian Li

BBa_K2721036

composite part

HRP with SnoopTag and SdyCatcher

1558

Yunqian Li

Best New Basic Part

BBa_k2721010

Curli is the major component of extra-cellular matrix produced by certain strains of Enterobacteriaceae. This part codes for CsgA, the monomer of Curli fibers which is an ideal material of biofilm. Click here to see more details.


Best New Composite Part

BBa_k2721036

This part codes for HRP(Horseradish Peroxidase), SnoopTag and SdyCatcher. Target DNA sequence of this part was optimized and synthesized. We cloned the synthesized sequence into vector pET-26b(+) and successfully expressed the target protein. Click here to see more details.


Best Part Collection

BBa_k2721027~BBa_k2721029

The main biofunction of our final product lies in the three connected enzymes. So the best part collection codes for the three enzymes LDH(Lactate Dehydrogenase), GOx (Glucose Oxidase) and HRP(Horseradish Peroxidase) separately flanked by different tag-catcher pairs. Click here to see more details.


Improve T7 promoters

BBa_k2721000~BBa_k2721003

Known for its highly yields of recombinant proteins, T7 promoter is currently one of the most widely used promoters in genetic engineering. This year, we did site-directed mutagenesis to get some mutants with even higher efficiency of protein expression. We chose GFP as a reporter to test the function of our modified T7 promoters. Click here to see more details.