Difference between revisions of "Team:DTU-Denmark/Experiments"

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  <tr>
 
    <th colspan="4"  style="background-color:#27518e;color:#ffffff;text-align:center;">MEA Media</th>
 
  </tr>
 
  <tr>
 
    <td class="tg-mhe8">Materials</td>
 
    <td class="tg-0lax">Weight (g)</td>
 
    <td class="tg-0lax">Weight (g)</td>
 
    <td class="tg-0lax">Weight (g)</td>
 
  </tr>
 
  <tr>
 
    <td class="tg-mhe8">Malt extract</td>
 
    <td class="tg-0lax">47.6</td>
 
    <td class="tg-0lax">49.9</td>
 
    <td class="tg-0lax">52.5</td>
 
  </tr>
 
  <tr>
 
    <td class="tg-mhe8">ZnSO4·7H2O</td>
 
    <td class="tg-0lax">0.01</td>
 
    <td class="tg-0lax">0.012</td>
 
    <td class="tg-0lax">0.01</td>
 
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    <td class="tg-mhe8">CuSO4·5H2O</td>
 
    <td class="tg-0lax">0.05</td>
 
    <td class="tg-0lax">0.049</td>
 
    <td class="tg-0lax">0.051</td>
 
  </tr>
 
  <tr>
 
    <td class="tg-mhe8">Agar</td>
 
    <td class="tg-0lax">14.99</td>
 
    <td class="tg-0lax">14.99</td>
 
    <td class="tg-0lax">15.04</td>
 
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    <th style="background-color:#27518e;color:#ffffff;text-align:center;" colspan="4">PDB Media</th>
 
  </tr>
 
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    <td class="tg-mhe8">Potato dextrose extract</td>
 
    <td class="tg-0lax">Weight (g)</td>
 
    <td class="tg-0lax">Weight (g)</td>
 
    <td class="tg-0lax">Weight (g)</td>
 
  </tr>
 
  <tr>
 
    <td class="tg-mhe8">Malt extract </td>
 
    <td class="tg-0lax">19.54</td>
 
    <td class="tg-0lax">39.12</td>
 
    <td class="tg-0lax">58.56</td>
 
  </tr>
 
  <tr>
 
    <td class="tg-mhe8">ZnSO4·7H2O</td>
 
    <td class="tg-0lax">0.012</td>
 
    <td class="tg-0lax">0.012</td>
 
    <td class="tg-0lax">0.0107</td>
 
  </tr>
 
  <tr>
 
    <td class="tg-mhe8">CuSO4·5H2O </td>
 
    <td class="tg-0lax">0.0056</td>
 
    <td class="tg-0lax">0.0053</td>
 
    <td class="tg-0lax">0.0049</td>
 
  </tr>
 
  <tr>
 
    <td class="tg-mhe8">Agar</td>
 
    <td class="tg-0lax">15.02</td>
 
    <td class="tg-0lax">15.04</td>
 
    <td class="tg-0lax">15.04</td>
 
  </tr>
 
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Revision as of 08:31, 14 September 2018

Experiments

All media was prepared before 11 am to be autoclaved. The actual weights of the different media was:

All media were stirred with a magnetic stirrer at around 110 degrees Celsius and 1000 rpm until homologous.
The species available (Pleuratus ostreatus) was inoculated on the solid PDA and MEA agar plates with a sterile toothpick under a fume hood and left at 30 degrees Celsius. It was on Monday discovered that the sample given was of mycelia without any spores and the process was redone.