Difference between revisions of "Team:Mingdao/Description"

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             <h2>Thioredoxin fusion system</h2>
 
             <h2>Thioredoxin fusion system</h2>
 
             <p style="text-indent:2em"> To create a reporter system, we constructed a GFP expression vector. We amplified a constitutive promoter from Drosophila actin 5c gene and an eukaryotic poly A signal by PCR from pAc5.1 vector. The resulting DNA fragments were assembled with a BioBrick existing part of GFP to generate the reporter vector of Ac5-GFP-polyA / pSB1C3 (K2543004).</p>
 
             <p style="text-indent:2em"> To create a reporter system, we constructed a GFP expression vector. We amplified a constitutive promoter from Drosophila actin 5c gene and an eukaryotic poly A signal by PCR from pAc5.1 vector. The resulting DNA fragments were assembled with a BioBrick existing part of GFP to generate the reporter vector of Ac5-GFP-polyA / pSB1C3 (K2543004).</p>
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             <img src="https://static.igem.org/mediawiki/2018/d/d5/T--Mingdao--project_picture_1.jpg" alt=""style="width:90%">
 
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Revision as of 10:01, 15 September 2018

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Design

Thioredoxin fusion system

To create a reporter system, we constructed a GFP expression vector. We amplified a constitutive promoter from Drosophila actin 5c gene and an eukaryotic poly A signal by PCR from pAc5.1 vector. The resulting DNA fragments were assembled with a BioBrick existing part of GFP to generate the reporter vector of Ac5-GFP-polyA / pSB1C3 (K2543004).




Reference

Antidote

Test Strip

Kit