Samdong2017 (Talk | contribs) |
Samdong2017 (Talk | contribs) |
||
Line 18: | Line 18: | ||
<script src="http://igem2017.worldshaper.cn/XSHS/js/jquery.fancybox.js"></script> | <script src="http://igem2017.worldshaper.cn/XSHS/js/jquery.fancybox.js"></script> | ||
<script src="http://igem2017.worldshaper.cn/XSHS/js/main.js"></script> | <script src="http://igem2017.worldshaper.cn/XSHS/js/main.js"></script> | ||
+ | |||
<header id="top-bar" class="navbar-fixed-top animated-header"> | <header id="top-bar" class="navbar-fixed-top animated-header"> | ||
<div class="container"> | <div class="container"> | ||
Line 28: | Line 29: | ||
</button> | </button> | ||
<div class="navbar-brand"> | <div class="navbar-brand"> | ||
− | <a href="https:// | + | <a href="https://2018.igem.org/Team:HFLS_ZhejiangUnited" > |
HFLS_ZhejiangUnited | HFLS_ZhejiangUnited | ||
</a> | </a> | ||
Line 37: | Line 38: | ||
<ul class="nav navbar-nav navbar-right"> | <ul class="nav navbar-nav navbar-right"> | ||
<li> | <li> | ||
− | <a href="https:// | + | <a href="https://2018.igem.org/Team:HFLS_ZhejiangUnited">Home</a> |
</li> | </li> | ||
<li class="dropdown"> | <li class="dropdown"> | ||
Line 47: | Line 48: | ||
<ul> | <ul> | ||
<li> | <li> | ||
− | <a href="https:// | + | <a href="https://2018.igem.org/Team:HFLS_ZhejiangUnited/aboutus.html">about us</a> |
</li> | </li> | ||
<li> | <li> | ||
− | <a href="https:// | + | <a href="https://2018.igem.org/Team:HFLS_ZhejiangUnited/members.html">Members</a> |
</li> | </li> | ||
<li> | <li> | ||
− | <a href="https:// | + | <a href="https://2018.igem.org/Team:HFLS_ZhejiangUnited/Collaborations">Collaborations</a> |
</li> | </li> | ||
</ul> | </ul> | ||
Line 66: | Line 67: | ||
<ul> | <ul> | ||
<li> | <li> | ||
− | <a href="https:// | + | <a href="https://2018.igem.org/Team:HFLS_ZhejiangUnited/Background.html">Background</a> |
</li> | </li> | ||
<li> | <li> | ||
− | <a href="https:// | + | <a href="https://2018.igem.org/Team:HFLS_ZhejiangUnited/design.html">Design</a> |
</li> | </li> | ||
<li> | <li> | ||
− | <a href="https:// | + | <a href="https://2018.igem.org/Team:HFLS_ZhejiangUnited/Protocols-methods.html">Protocols and methods</a> |
</li> | </li> | ||
<li> | <li> | ||
− | <a href="https:// | + | <a href="https://2018.igem.org/Team:HFLS_ZhejiangUnited/result.html">Results</a> |
</li> | </li> | ||
<li> | <li> | ||
− | <a href="https:// | + | <a href="https://2018.igem.org/Team:HFLS_ZhejiangUnited/Future-plans.html">Future plans</a> |
</li> | </li> | ||
<li> | <li> | ||
− | <a href="https:// | + | <a href="https://2018.igem.org/Team:HFLS_ZhejiangUnited/Attributions">Attributions</a> |
</li> | </li> | ||
<li> | <li> | ||
− | <a href="https:// | + | <a href="https://2018.igem.org/Team:HFLS_ZhejiangUnited/InterLab">Interlab</a> |
</li> | </li> | ||
<li> | <li> | ||
− | <a href="https:// | + | <a href="https://2018.igem.org/Team:HFLS_ZhejiangUnited/Model">Model</a> |
</li> | </li> | ||
<li> | <li> | ||
− | <a href="https:// | + | <a href="https://2018.igem.org/Team:HFLS_ZhejiangUnited/Demonstrate">Demonstrate</a> |
</li> | </li> | ||
Line 97: | Line 98: | ||
</li> | </li> | ||
<li> | <li> | ||
− | <a href="https:// | + | <a href="https://2018.igem.org/Team:HFLS_ZhejiangUnited/parts.html">Parts</a> |
</li> | </li> | ||
<li class="dropdown"> | <li class="dropdown"> | ||
Line 107: | Line 108: | ||
<ul> | <ul> | ||
<li> | <li> | ||
− | <a href="https:// | + | <a href="https://2018.igem.org/Team:HFLS_ZhejiangUnited/HP/Silver">Silver</a> |
</li> | </li> | ||
<li> | <li> | ||
− | <a href="https:// | + | <a href="https://2018.igem.org/Team:HFLS_ZhejiangUnited/HP/Gold_Integrated">Integrated and Gold</a> |
</li> | </li> | ||
<li> | <li> | ||
− | <a href="https:// | + | <a href="https://2018.igem.org/Team:HFLS_ZhejiangUnited/Engagement">Engagement</a> |
</li> | </li> | ||
</ul> | </ul> | ||
Line 119: | Line 120: | ||
</li> | </li> | ||
<li> | <li> | ||
− | <a href="https:// | + | <a href="https://2018.igem.org/Team:HFLS_ZhejiangUnited/Applied_Design">Applied Design</a> |
</li> | </li> | ||
<li class="dropdown"> | <li class="dropdown"> | ||
Line 129: | Line 130: | ||
<ul> | <ul> | ||
<li> | <li> | ||
− | <a href="https:// | + | <a href="https://2018.igem.org/Team:HFLS_ZhejiangUnited/Medal-requirements.html">Medal requirements</a> |
</li> | </li> | ||
</ul> | </ul> | ||
Line 135: | Line 136: | ||
</li> | </li> | ||
<li> | <li> | ||
− | <a href="https:// | + | <a href="https://2018.igem.org/Team:HFLS_ZhejiangUnited/safety.html">Safety</a> |
</li> | </li> | ||
</ul> | </ul> |
Revision as of 14:56, 26 September 2018
Hometown Hangzhou is widely known by water- related UNESCO World Heritages – West Lake and Grand Canal, however, severe water pollution problem caused by many reasons also exist. Hence, we hope to provide an easy-operating and low-cost tool for public to monitor water quality around.
We designed a portable water quality bio-detector prototype based on E.coli strains which were constructed to detect dissolved oxygen, phosphorus or nitrogen in water respectively. The oxygen sensitive vgb promoter and a GFP reporter constitute Oxygen detector 1.0. To enhance the expression of GFP, version 2.0 contains a vgb promoter, a T7 RNA polymerase gene, a T7 promoter and a GFP gene. For nitrogen, the PyeaR promoter was used to response to different concentrations of nitrate, nitrite and nitric, with a BFP reporter gene. For phosphate, a plasmid consisting of an ‘external phosphate sensing promoter’ to sense the phosphate concentration and a RFP gene to report.
Abstract