Difference between revisions of "Team:FJNU-China/InterLab"
| Line 244: | Line 244: | ||
<hr> | <hr> | ||
<h2> Materials</h2> | <h2> Materials</h2> | ||
| + | <p ><span style="font-size:20px;font-weight:bold;">Strain used</span></br><span style=" font-style:italic;">Escherichia coli</span> DH5α</p> | ||
| + | <p ><span style="font-size:20px;font-weight:bold;">Plasmid used</span></br>Negative control: BBa_R0040 Plate 7 Well 2D </br> | ||
| + | Positive control: BBa_I20270 Plate 7 Well 2B </br> | ||
| + | Test Device 1: BBa_J364000 Plate 7 Well 2F </br> | ||
| + | Test Device 2: BBa_J364001 Plate 7 Well 2H </br> | ||
| + | Test Device 3: BBa_J364002 Plate 7 Well 2J </br> | ||
| + | Test Device 4: BBa_J364007 Plate 7 Well 2L </br> | ||
| + | Test Device 5: BBa_J364008 Plate 7 Well 2N <br> | ||
| + | Test Device 6: BBa_J364009 Plate 7 Well 2P | ||
| + | </p> | ||
| + | <p ><span style="font-size:20px;font-weight:bold;">Materials</span></br>Measurement Kit ( 1ml LUDOX CL-X, 150 μL Silica Bead, Fluorescein) </br> | ||
| + | 1x PBS</br> | ||
| + | ddH2O </br> | ||
| + | LB media </br> | ||
| + | Chloramphenicol (stock concentration 25 mg/mL dissolved in EtOH) </br> | ||
| + | 50 ml Falcon tube (or equivalent, preferably amber or covered in foil to block light) </br> | ||
| + | 96 well plates, black with clear flat bottom preferred | ||
| + | </p> | ||
<p ><span style="font-size:20px;font-weight:bold;">Strain used</span></br><span style=" font-style:italic;">Escherichia coli</span> DH5α</p> | <p ><span style="font-size:20px;font-weight:bold;">Strain used</span></br><span style=" font-style:italic;">Escherichia coli</span> DH5α</p> | ||
<p>Vestibulum consectetur scelerisque lacus, ac fermentum lorem convallis sed. Nam odio tortor, dictum quis malesuada at, pellentesque vitae orci. Vivamus elementum, felis eu auctor lobortis, diam velit egestas lacus, quis fermentum metus ante quis urna. Sed at facilisis libero. Cum sociis natoque penatibus et magnis dis parturient montes, nascetur ridiculus mus. Vestibulum bibendum blandit dolor. Nunc orci dolor, molestie nec nibh in, hendrerit tincidunt ante. Vivamus sem augue, hendrerit non sapien in, mollis ornare augue.</p> | <p>Vestibulum consectetur scelerisque lacus, ac fermentum lorem convallis sed. Nam odio tortor, dictum quis malesuada at, pellentesque vitae orci. Vivamus elementum, felis eu auctor lobortis, diam velit egestas lacus, quis fermentum metus ante quis urna. Sed at facilisis libero. Cum sociis natoque penatibus et magnis dis parturient montes, nascetur ridiculus mus. Vestibulum bibendum blandit dolor. Nunc orci dolor, molestie nec nibh in, hendrerit tincidunt ante. Vivamus sem augue, hendrerit non sapien in, mollis ornare augue.</p> | ||
Revision as of 06:52, 30 September 2018
Interlab
Overview
Reliable and repeatable measurement is a key component to all engineering disciplines. However, the number of cells in the sample is a variability in measurements. The goal of the iGEM InterLab Study is to identify and correct the sources of systematic variability in synthetic biology measurements. This year, our team take part in the fifth InterLab study, using normalizing to absolute cell count or CFUs instead of OD to reduce variability of fluorescence measurements.
Materials
Strain usedEscherichia coli DH5α
Plasmid usedNegative control: BBa_R0040 Plate 7 Well 2D
Positive control: BBa_I20270 Plate 7 Well 2B
Test Device 1: BBa_J364000 Plate 7 Well 2F
Test Device 2: BBa_J364001 Plate 7 Well 2H
Test Device 3: BBa_J364002 Plate 7 Well 2J
Test Device 4: BBa_J364007 Plate 7 Well 2L
Test Device 5: BBa_J364008 Plate 7 Well 2N
Test Device 6: BBa_J364009 Plate 7 Well 2P
MaterialsMeasurement Kit ( 1ml LUDOX CL-X, 150 μL Silica Bead, Fluorescein) 1x PBS ddH2O LB media Chloramphenicol (stock concentration 25 mg/mL dissolved in EtOH) 50 ml Falcon tube (or equivalent, preferably amber or covered in foil to block light) 96 well plates, black with clear flat bottom preferred
Strain usedEscherichia coli DH5α
Vestibulum consectetur scelerisque lacus, ac fermentum lorem convallis sed. Nam odio tortor, dictum quis malesuada at, pellentesque vitae orci. Vivamus elementum, felis eu auctor lobortis, diam velit egestas lacus, quis fermentum metus ante quis urna. Sed at facilisis libero. Cum sociis natoque penatibus et magnis dis parturient montes, nascetur ridiculus mus. Vestibulum bibendum blandit dolor. Nunc orci dolor, molestie nec nibh in, hendrerit tincidunt ante. Vivamus sem augue, hendrerit non sapien in, mollis ornare augue.
Result
Integer pulvinar leo id risus pellentesque vestibulum. Sed diam libero, sodales eget sapien vel, porttitor bibendum enim. Donec sed nibh vitae lorem porttitor blandit in nec ante.
Integer pulvinar leo id risus pellentesque vestibulum. Sed diam libero, sodales eget sapien vel, porttitor bibendum enim. Donec sed nibh vitae lorem porttitor blandit in nec ante.
Integer pulvinar leo id risus pellentesque vestibulum. Sed diam libero, sodales eget sapien vel, porttitor bibendum enim. Donec sed nibh vitae lorem porttitor blandit in nec ante.
Integer pulvinar leo id risus pellentesque vestibulum. Sed diam libero, sodales eget sapien vel, porttitor bibendum enim. Donec sed nibh vitae lorem porttitor blandit in nec ante.
Analyse
Suspendisse a orci facilisis, dignissim tortor vitae, ultrices mi. Vestibulum a iaculis lacus. Phasellus vitae convallis ligula, nec volutpat tellus. Vivamus scelerisque mollis nisl, nec vehicula elit egestas a. Sed luctus metus id mi gravida, faucibus convallis neque pretium. Maecenas quis sapien ut leo fringilla tempor vitae sit amet leo. Donec imperdiet tempus placerat. Pellentesque pulvinar ultrices nunc sed ultrices. Morbi vel mi pretium, fermentum lacus et, viverra tellus. Phasellus sodales libero nec dui convallis, sit amet fermentum sapien auctor. Vestibulum ante ipsum primis in faucibus orci luctus et ultrices posuere cubilia Curae; Sed eu elementum nibh, quis varius libero.