Difference between revisions of "Team:SSTi-SZGD"

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Abstract
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Now is a younger world, more people pay attention to their appearance, and the development of cosmetics is increasing.
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<!--{cn}现在是一个年轻的世界，越来越多的人注重自己的外表，并且化妆品的发展日益增加。-->
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The production of hyaluronic acid has been changed from traditional animal tissue formulation to microbial fermentation. However, there is no report that tissue cells or microorganisms can di-rectly produce low molecular weight hyaluronic acid. In order to prepare low molecular weight hyaluronic acid, physical and chemical methods are needed. However, there are many drawbacks in physical and chemical methods, such as poor product stability, low efficiency, complex reac-tion conditions and possible environmental pollution.
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<!--{cn}透明质酸的生产已经从传统的动物组织配方转变为微生物发酵，但目前还没有关于组织细胞或微生物可以直接生产低分子透明质酸的报道，为了制备低分子透明质酸，需要采用物理和化学的方法，但在物化方法上存在着产品稳定性差、效率低、反应条件复杂、可能对环境造成污染等问题。-->
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This year our project constructed a recombinant strain Bacillus subtilis 168E which could directly produce different molecular weight hyaluronic acid products by regulating the activities of LHAase. Bacillus subtilis 168 was selected as the best chassis. The HasA gene and identified pre-cursor  genes (tuaD , gtaB , gluM , glmM and glmS) was transferred into Bacillus subtilis. Since hyaluronic acid of high molecular weight was produced at this time, we transferred the LHAase gene into Bacillus subtilis 168 which is from leech resources coding hyaluronidase. Therefore the hyaluronic acid could be enzymatic hydrolyzed to different molecular weight.
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<!--{cn}因此，我们打算构建一株能直接产生低分子透明质酸的菌株，发现枯草芽孢杆菌168为最佳选择，将hsA基因转入枯草芽孢杆菌168，并对透明质酸途径基因进行优化，使其大量产生透明质酸。由于此时产生了高分子量的透明质酸，我们将该基因转入枯草杆菌。最后，我们获得了一株能直接生产低分子透明质酸产品的重组枯草芽孢杆菌168 E。-->
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Our intended application is use low molecular weight hyaluronic acid to prepare microneedles and that hyaluronic acid can be dissolved and stuck in the skin under the action of body fluid when it is punctured into the human skin, microneedles allow the penetration of low molecular weight HA through to the dermal papillary cutis, which could not only play a cosmetic role. Be-cause hyaluronic acid is an endogenous substance of the human body and does not cause the in-flammatory reaction, therefore it is a new type of microneedle material and has a potential mar-ket.
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<!--{cn}通过查阅文献，我们认为低分子量的透明质酸可以作为制备微针的基质，当透明质酸被刺入人体皮肤时，透明质酸可以在体液的作用下溶解和附着在皮肤中，这不仅可以起到美容作用，因为透明质酸是人体内的一种内源性物质，而不是引起人体内源性物质的原因。炎症反应，是一种新型的微针材料，具有潜在的市场前景。-->
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The most common substance in cosmetics is hyaluronic acid, which has a good moisturizing ability. And it can remove wrinkles that can make people look younger.
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<!--{cn}化妆品中最常见的物质是透明质酸，具有良好的保湿能力。 它可以去除皱纹，使人看起来更年轻。-->
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General use streptococus to produce HA but it have risk of infection.So we construct a recombinant Bacillus subtilis strain to produce high molecular weight hyaluronic acid.
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<!--{cn}一般使用兽疫链球菌产生HA，但它有感染的风险。因此我们构建了重组枯草芽孢杆菌菌株以产生高分子量透明质酸。-->
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Only Low molecular weight hyaluronic acid has stable structure after cross-linking and can be made for a cosmetic.So we recombinant our strain again,make it can produce LMW-HA.
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<!--{cn}只有低分子量的透明质酸在交联后才具有稳定的结构，可以制成化妆品。所以我们再次重组我们的菌株，使其能够生产LMW-HA。-->
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Hyaluronic Acid Micro-Needles Patch !!!
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<!--{cn}透明质酸微针贴片!!!-->
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<a href="https://2018.igem.org/Team:SSTi-SZGD/Description">Project Detail<!--{cn}项目详情--></a>
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Revision as of 09:20, 1 October 2018

Hyaluronic acid micro factory : A bacterium produces low molecular weight hyaluronic acid
Engineering strain : Bacillus subtilis
Genetic origin : Streptococcus equi and Leech
read more
Now is a younger world, more people pay attention to their appearance, and the development of cosmetics is increasing.
The most common substance in cosmetics is hyaluronic acid, which has a good moisturizing ability. And it can remove wrinkles that can make people look younger.
General use streptococus to produce HA but it have risk of infection.So we construct a recombinant Bacillus subtilis strain to produce high molecular weight hyaluronic acid.
Only Low molecular weight hyaluronic acid has stable structure after cross-linking and can be made for a cosmetic.So we recombinant our strain again,make it can produce LMW-HA.
Hyaluronic Acid Micro-Needles Patch !!!
Project Detail

Copyright © 2018 SSTi-SZGD All Rights Reserved.
Copyright © 2018 SSTi-SZGD All Rights Reserved.