Difference between revisions of "Team:Kyoto/Safety"

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<h5 id="Safe project design">1) Safe project design</h5>
 
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<h6><a href="https://2017.igem.org/Team:Kyoto/Basic_Part" target="brank">Basic Parts</a></h6>
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<p>All of the species we expect to use are not harmful for human.</p>
 
<h6><a href="https://2017.igem.org/Team:Kyoto/Composite_Part" target="brank">Composite Parts</a></h6>
 
<h6><a href="https://2017.igem.org/Team:Kyoto/Composite_Part" target="brank">Composite Parts</a></h6>
 
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Revision as of 08:27, 2 October 2018

Safety






1) Safe project design

All of the species we expect to use are not harmful for human.

Composite Parts
2) Safe lab work
primer namesequenceLengthTmGC%DesignerManufacturer
tropomyosinFGATCGAGAAGGACAACGCCC206560FukudaMacrogen Japan Corp
IK107cccgccgccaccatggaggcggccgcaaaatcagg359471YoshimotoMacrogen Japan Corp
3) Safe shipment
3-1 Kit
NameSupplier
Wizard® SV Gel and PCRPromega
FastGene™Plasmid Mini KitNIPPON Genetics Co.,Ltd
4-1 Miniprep

Minipreps were performed using FastGene™Plasmid Mini Kit and and Wizard® Plus SV Minipreps DNA Purification System according to the manufacturer's protocols.

4-17 Soaking

We followed this paper as for soaking.
https://link.springer.com/content/pdf/10.1007%2Fs10658-012-0035-0.pdf





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