Difference between revisions of "Team:SSHS-Shenzhen/InterLab"
| Line 102: | Line 102: | ||
</h1> | </h1> | ||
| + | <h2> | ||
| + | Monday, July 23, 2018 10:30 | ||
| + | </h2> | ||
| + | |||
| + | |||
| + | <p id="para"> | ||
| + | 1) Obtain the tube labeled “Silica Beads” from the InterLab test kit and vortex 4 vigorously for 30 seconds.<br> | ||
| + | 2) Immediately pipet 96 μL microspheres into a 1.5 mL eppendorf tube <br> | ||
| + | 3) Add 904 μL of ddH2O to the microspheres | ||
| + | </p> | ||
<br><br><br><br><br> | <br><br><br><br><br> | ||
</body> | </body> | ||
Revision as of 13:09, 2 October 2018
Monday July 23, 2018 11:30
7/23: > Calibration 1: OD600 Reference point
Monday, July 23, 2018 9:00
1) Add 100 μl LUDOX into wells A1, B1, C1, D1
2) Add 100 μl of dd H2O into wells A2, B2, C2, D2
3) Measure absorbance at 600 nm of all samples in the measurement mode we plan to use for cell measurements
4) Record the data in both the table below and our notebook
5) Import data into Excel sheet provided
Monday, July 23, 2018 9:25(pm)
Specific data from our experiment
| LUDOX CL-X | H2O | |
| Replicate 1 | 0.058 | 0.038 |
| Replicate 2 | 0.059 | 0.037 |
| Replicate 3 | 0.053 | 0.037 |
| Replicate 4 | 0.055 | 0.036 |
| Arith. Mean | 0.056 | 0.037 |
| Corrected Abs600 | 0.019 | |
| Reference OD600 | 0.063 | |
| OD600/Abs600 | 3.238 |
Note: Carefully pipet liquids into the wells of 96 well plates in order to prevent the case of liquid sticking on the wall of wells and caused inaccurate data.
7/23:> Calibration 2: Particle Standard Curve
Monday, July 23, 2018 10:30
1) Obtain the tube labeled “Silica Beads” from the InterLab test kit and vortex 4 vigorously for 30 seconds.
2) Immediately pipet 96 μL microspheres into a 1.5 mL eppendorf tube
3) Add 904 μL of ddH2O to the microspheres