Difference between revisions of "Team:SSHS-Shenzhen/InterLab"

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3) Add 904 μL of ddH2O to the microspheres  
 
3) Add 904 μL of ddH2O to the microspheres  
 
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Monday, July 23, 2018 10:40
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1）Add 100 μl of ddH​2​O​ into wells A2, B2, C2, D2....A12, B12, C12, D12 <br>
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2）Vortex the tube containing the stock solution of microspheres vigorously for 10 seconds <br>
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3）Immediately add 200 μl​ of microspheres stock​ solution into A1 <br>
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4）Transfer 100 μl of microsphere stock solution from A1 into A2. <br>
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5）Mix A2 by pipetting up and down 3x and transfer 100 μl into A3… <br>
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6）Mix A3 by pipetting up and down 3x and transfer 100 μl into A4...<br>
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7）Mix A4 by pipetting up and down 3x and transfer 100 μl into A5...<br>
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8）Mix A5 by pipetting up and down 3x and transfer 100 μl into A6... <br>
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9）Mix A6 by pipetting up and down 3x and transfer 100 μl into A7... <br>
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10）Mix A7 by pipetting up and down 3x and transfer 100 μl into A8... 5 <br>
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11）Mix A8 by pipetting up and down 3x and transfer 100 μl into A9... <br>
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12）Mix A9 by pipetting up and down 3x and transfer 100 μl into A10... <br>
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13）Mix A10 by pipetting up and down 3x and transfer 100 μl into A11... <br>
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14）Mix A11 by pipetting up and down 3x and transfer 100 μl into <b>liquid waste</b> <br>
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Revision as of 13:11, 2 October 2018

Title

Title
InterLab

Monday July 23, 2018 11:30

7/23: > Calibration 1:​ OD​600​ Reference point

Monday, July 23, 2018 9:00

1) Add 100 μl LUDOX into wells A1, B1, C1, D1
2) Add 100 μl of dd H2O into wells A2, B2, C2, D2
3) Measure absorbance at 600 nm of all samples in the measurement mode we plan to use for cell measurements
4) Record the data in both the table below and our notebook
5) Import data into Excel sheet provided

Monday, July 23, 2018 9:25(pm)

Specific data from our experiment


LUDOX CL-X H2O
Replicate 1 0.058 0.038
Replicate 2 0.059 0.037
Replicate 3 0.053 0.037
Replicate 4 0.055 0.036
Arith. Mean 0.056 0.037
Corrected Abs600 0.019
Reference OD600 0.063
OD600/Abs600 3.238

Note: Carefully pipet liquids into the wells of 96 well plates in order to prevent the case of liquid sticking on the wall of wells and caused inaccurate data.

7/23:> Calibration 2:​ Particle Standard Curve

Monday, July 23, 2018 10:30

1) Obtain the tube labeled “Silica Beads” from the InterLab test kit and vortex 4 vigorously for 30 seconds.
2) Immediately pipet 96 μL microspheres into a 1.5 mL eppendorf tube
3) Add 904 μL of ddH2O to the microspheres

Monday, July 23, 2018 10:40

1）Add 100 μl of ddH​2​O​ into wells A2, B2, C2, D2....A12, B12, C12, D12
2）Vortex the tube containing the stock solution of microspheres vigorously for 10 seconds
3）Immediately add 200 μl​ of microspheres stock​ solution into A1
4）Transfer 100 μl of microsphere stock solution from A1 into A2.
5）Mix A2 by pipetting up and down 3x and transfer 100 μl into A3…
6）Mix A3 by pipetting up and down 3x and transfer 100 μl into A4...
7）Mix A4 by pipetting up and down 3x and transfer 100 μl into A5...
8）Mix A5 by pipetting up and down 3x and transfer 100 μl into A6...
9）Mix A6 by pipetting up and down 3x and transfer 100 μl into A7...
10）Mix A7 by pipetting up and down 3x and transfer 100 μl into A8... 5
11）Mix A8 by pipetting up and down 3x and transfer 100 μl into A9...
12）Mix A9 by pipetting up and down 3x and transfer 100 μl into A10...
13）Mix A10 by pipetting up and down 3x and transfer 100 μl into A11...
14）Mix A11 by pipetting up and down 3x and transfer 100 μl into liquid waste