Difference between revisions of "Team:FJNU-China/Description"

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<h3 style="text-align:left;"><i class="fa fa-hand-o-right"  style=" align:center; color:tomato;" ></i>&nbsp;&nbsp;Design</h3>
 
<h3 style="text-align:left;"><i class="fa fa-hand-o-right"  style=" align:center; color:tomato;" ></i>&nbsp;&nbsp;Design</h3>
 
   <p>According to different scenarios, we designed different promoters to combine with mazF. With the humanization design, the biosafty of our projects is guaranteed. </p>
 
   <p>According to different scenarios, we designed different promoters to combine with mazF. With the humanization design, the biosafty of our projects is guaranteed. </p>
<h3 style="text-align:left;">&nbsp;&nbsp;&nbsp;&nbsp;Design</h3>
 
  
<h3 style="text-align:left;"><i class="fa fa-hand-o-right"   style=" align:center; color:tomato;" ></i>&nbsp;&nbsp;Design</h3>
+
<h3 style="text-align:left;">&nbsp;&nbsp;&nbsp;&nbsp;Bromidrosis</h3>
   <p>mazEF is a toxin-antitoxin module located on the Escherichia coli chromosome and that of some other bacteria, including pathogens. mazF specifies for a stable toxin, MazF, and mazE specifies for a labile antitoxin, MazE, that antagonizes MazF. (Engelberg-Kulka, H., et al. 2005)MazF is a toxic nuclease arresting cell growth through the mechanism of RNA cleavage and that MazE inhibits the RNase activity of MazF by forming a complex.(Syed, M. A., et al. 2011).We used mazF as our killer to ensure biosafty. </p>
+
  <p>In our scent spray, we designed a suicide switch induced by arabinose. When we don't want the engineered bacteria to work again, we can kill the engineered bacteria by spraying a spray containing arabinose. In order to ensure that our engineering bacteria do not work in other environments, we set the switch of PLA and 2-PE to salt control and temperature control. Only when the temperature reaches the body temperature condition and the salinity reaches the human sweating condition, our engineering bacteria will play a role. </p>
 +
<div class="col-sm-12 col-md-12">
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<img  style="width:99%"src="https://static.igem.org/mediawiki/2018/e/ee/T--FJNU-China--Description-mazEF.png" alt="mazEF">
 +
</div>
 +
<h3 style="text-align:left;">&nbsp;&nbsp;&nbsp;&nbsp;Garbage can</h3>
 +
   <p>In the trash can lid, we chose a light-controlled promoter to combine with mazF. Once the engineered bacteria leaves the dark environment of the trash can, it will be killed. </p>
 
<div class="col-sm-12 col-md-12">
 
<div class="col-sm-12 col-md-12">
 
<img  style="width:99%"src="https://static.igem.org/mediawiki/2018/e/ee/T--FJNU-China--Description-mazEF.png" alt="mazEF">
 
<img  style="width:99%"src="https://static.igem.org/mediawiki/2018/e/ee/T--FJNU-China--Description-mazEF.png" alt="mazEF">
 
</div>
 
</div>
 
<h3 style="text-align:left;">&nbsp;&nbsp;&nbsp;&nbsp;Bromidrosis</h3>
 
  <p>According to different scenarios, we designed different promoters to combine with mazF. With the humanization design, the biosafty of our projects is guaranteed. </p>
 
  
  

Revision as of 15:12, 2 October 2018

Description

  • Abstract
  • PLA
  • 2-PE
  • Garbage can
  • Bromidrosis
  • Biosafty
  • Demonstrate

Abstract

As we know, the purpose of modeling is mainly to provide guidance and solve problems for the team experiment. So, before we designed the model, we sorted out some of the problems the team was facing. Based on these three problems,.Because The effect of PLA concentration on the quantity Staphylococcus epidermidis.Vestibulum quis quam ut magna consequat faucibus. Pellentesque eget nisi a mi suscipit tincidunt. Ut tempus dictum risus. Pellentesque viverra sagittis quam at mattis. Suspendisse potenti. Aliquam sit amet gravida nibh, facilisis gravida odio. Phasellus auctor velit at lacus blandit, commodo iaculis justo viverra. Etiam vitae est arcu.

PLA

As we know, the purpose of modeling is mainly to provide guidance and solve problems for the team experiment. So, before we designed the model, we sorted out some of the problems the team was facing. Based on these three problems,.Because The effect of PLA concentration on the quantity Staphylococcus epidermidis.Vestibulum quis quam ut magna consequat faucibus. Pellentesque eget nisi a mi suscipit tincidunt. Ut tempus dictum risus. Pellentesque viverra sagittis quam at mattis. Suspendisse potenti. Aliquam sit amet gravida nibh, facilisis gravida odio. Phasellus auctor velit at lacus blandit, commodo iaculis justo viverra. Etiam vitae est arcu.

2-PE

As we know, the purpose of modeling is mainly to provide guidance and solve problems for the team experiment. So, before we designed the model, we sorted out some of the problems the team was facing. Based on these three problems,.Because The effect of PLA concentration on the quantity Staphylococcus epidermidis.Vestibulum quis quam ut magna consequat faucibus. Pellentesque eget nisi a mi suscipit tincidunt. Ut tempus dictum risus. Pellentesque eget nisi a mi suscipit tincidunt. Ut tempus dictum risus.

Garbage can

As we know, the purpose of modeling is mainly to provide guidance and solve problems for the team experiment. So, before we designed the model, we sorted out some of the problems the team was facing. Based on these three problems,.Because The effect of PLA concentration on the quantity Staphylococcus epidermidis.Vestibulum quis quam ut magna consequat faucibus. Pellentesque eget nisi a mi suscipit tincidunt. Ut tempus dictum risus. Pellentesque viverra sagittis quam at mattis. Suspendisse potenti. Aliquam sit amet gravida nibh, facilisis gravida odio. Phasellus auctor velit at lacus blandit, commodo iaculis justo viverra. Etiam vitae est arcu.

Bromidrosis

As we know, the purpose of modeling is mainly to provide guidance and solve problems for the team experiment. So, before we designed the model, we sorted out some of the problems the team was facing. Based on these three problems,.Because The effect of PLA concentration on the quantity Staphylococcus epidermidis.Vestibulum quis quam ut magna consequat faucibus. Pellentesque eget nisi a mi suscipit tincidunt. Ut tempus dictum risus. Pellentesque viverra sagittis quam at mattis. Suspendisse potenti. Aliquam sit amet gravida nibh, facilisis gravida odio. Phasellus auctor velit at lacus blandit, commodo iaculis justo viverra. Etiam vitae est arcu.

Biosafty

  Background

mazEF is a toxin-antitoxin module located on the Escherichia coli chromosome and that of some other bacteria, including pathogens. mazF specifies for a stable toxin, MazF, and mazE specifies for a labile antitoxin, MazE, that antagonizes MazF. (Engelberg-Kulka, H., et al. 2005)MazF is a toxic nuclease arresting cell growth through the mechanism of RNA cleavage and that MazE inhibits the RNase activity of MazF by forming a complex.(Syed, M. A., et al. 2011).We used mazF as our killer to ensure biosafty.

mazEF

  Design

According to different scenarios, we designed different promoters to combine with mazF. With the humanization design, the biosafty of our projects is guaranteed.

    Bromidrosis

In our scent spray, we designed a suicide switch induced by arabinose. When we don't want the engineered bacteria to work again, we can kill the engineered bacteria by spraying a spray containing arabinose. In order to ensure that our engineering bacteria do not work in other environments, we set the switch of PLA and 2-PE to salt control and temperature control. Only when the temperature reaches the body temperature condition and the salinity reaches the human sweating condition, our engineering bacteria will play a role.

mazEF

    Garbage can

In the trash can lid, we chose a light-controlled promoter to combine with mazF. Once the engineered bacteria leaves the dark environment of the trash can, it will be killed.

mazEF

Demonstrate

EurAsian Meetup

As we know, the purpose of modeling is mainly to provide guidance and solve problems for the team experiment. So, before we designed the model, we sorted out some of the problems the team was facing. Based on these three problems,.Because The effect of PLA concentration on the quantity Staphylococcus epidermidis.Vestibulum quis quam ut magna consequat faucibus. Pellentesque eget nisi a mi suscipit tincidunt. Ut tempus dictum risus. Pellentesque viverra sagittis quam at mattis. Suspendisse potenti. Aliquam sit amet gravida nibh, facilisis gravida odio. Phasellus auctor velit at lacus blandit, commodo iaculis justo viverra. Etiam vitae est arcu.

5th CCiC

As we know, the purpose of modeling is mainly to provide guidance and solve problems for the team experiment. So, before we designed the model, we sorted out some of the problems the team was facing. Based on these three problems,.Because The effect of PLA concentration on the quantity Staphylococcus epidermidis.Vestibulum quis quam ut magna consequat faucibus. Pellentesque eget nisi a mi suscipit tincidunt. Ut tempus dictum risus. Pellentesque viverra sagittis quam at mattis. Suspendisse potenti. Aliquam sit amet gravida nibh, facilisis gravida odio. Phasellus auctor velit at lacus blandit, commodo iaculis justo viverra. Etiam vitae est arcu.