Difference between revisions of "Team:Navarra BG/safety"

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<h2 id="scroll-to"><span>Safety</span></h2>
 
<h2 id="scroll-to"><span>Safety</span></h2>
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<p class="mt-30" style=" margin-bottom: 30px; "> Lorem ipsum dolor sit amet, consectetur adipiscing elit. Sed in hendrerit leo, vitae elementum neque. Sed ullamcorper sapien nec lectus sagittis, non tempus leo venenatis. Nam suscipit, eros vel facilisis sollicitudin, justo leo varius elit, ut suscipit diam tellus ac turpis. Praesent ex eros, feugiat non rutrum at, semper eleifend turpis. Donec vitae dolor vel lorem dapibus fringilla et nec dui. Etiam felis urna, tristique a ipsum eget, pulvinar vulputate enim. Nulla mollis sit amet ante porttitor mattis. Nam vel metus eget ipsum porttitor interdum et vel risus.</p>
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<p>Working a team of eight people in a laboratory is a very nice experience, very enriching, fun and, above all, it must be very safe. Since the project was raised we were clear that we are a High School team and that the important thing is the protection. Therefore, decisions were made about the design of the project to reduce possible risks to the maximum, how to choose  on-pathogenic microorganisms and use all the necessary protective material.</p>
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<p>Our project is based on developing a method for the purification of recombinant proteins in plants, simple and profitable, which has been developed with the Carbohydrate Metabolism group (belonging to the CSIC) in its laboratories at the Institute of Agrobiotechnology.</p>
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<p>The laboratory where it has been developed el proyecto, tiene un nivel de bioseguridad 1 (low risk). Además, los organismos con los que trabajamos no presentan riesgo potencial.</p>
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<p>However, during the entire time we were in the laboratory, we met the following security measures:</p>
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<img class="img-fluid d-flex mx-auto" src="https://static.igem.org/mediawiki/2018/e/eb/T--Navarra_BG--LOGOOOOO.jpg" alt="BioGalaxy">
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<p><b>Supervised work</b></p>
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<p>At all times we have been supervised by the people in charge of the laboratory, who have been in charge of supervising the actions carried out and guiding us in the appropriate knowledge and techniques.</p>
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<p>Before entering the laboratory, we attended mandatory lectures on Health and Biosafety in the laboratory, where they also explained the protocols and actions to follow in case of emergency.</p>
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<p><b>Prevention and safety measures</b></p>
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<p>We receive lab coats (each person with their own gown), safety goggles, respirators and nitrile and / or latex gloves.</p>
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<p>We had our own workspace, dedicated to the development of our project; and that we always kept clean and tidy.</p>
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<p>To reduce any type of risk, when we worked, we disinfected the Ethanol work area before and after each use.</p>
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<div class="img-text">
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<p>The laboratory has a biological safety cabinet in which we managed the bacteria to avoid external contamination.</p>
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<img src="img/a.jpg" alt="" class="img-fluid float-left mr-20 mb-20">
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<p>Any waste that we produced was deposited in its corresponding container and processed in the regulatory manner.</p>
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<p>Recently, the US Federal government banned online casinos from operating in America by making it illegal to transfer money to them through any US bank or payment system. As a result of this law, most of the popular online casino networks such as Party Gaming and PlayTech left the United States. Overnight, online casino players found themselves being chased by the Federal government. But, after a fortnight, the online casino industry came up with a solution and new online casinos started taking root. These began to operate under a different business umbrella, and by doing that, rendered the transfer of money to and from them legal. A major part of this was enlisting electronic banking systems that would accept this new clarification and start doing business with me. Listed in this article are the electronic banking systems that accept players from the United States that wish to play in online casinos.</p>
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<p>In addition, Ethidium Bromide has been replaced in the laboratory by Midori Green, a compound with many fewer risks than the previous one.</p>
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<p><b>Organisms</b></p>
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<p>Recently, the US Federal government banned online casinos from operating in America by making it illegal to transfer money to them through any US bank or payment system. As a result of this law, most of the popular online casino networks such as Party Gaming and PlayTech left the United States. Overnight, online casino players found themselves being chased by the Federal government. But, after a fortnight, the online casino industry came up with a solution and new online casinos started taking root. These began to operate under a different business umbrella, and by doing that, rendered the transfer of money to and from them legal. A major part of this was enlisting electronic banking systems that would accept this new clarification and start doing business with me. Listed in this article are the electronic banking systems that accept players from the United States that wish to play in online casinos.</p>
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<p>To choose well the design of the project depended to avoid many possible biological risks. That is why it was decided to work with non-pathogenic organisms, which have no potential risk outside the laboratory, neither for humans, animals or the environment.</p>
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<p>These organisms are:</p>
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<ul>
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<p>Recently, the US Federal government banned online casinos from operating in America by making it illegal to transfer money to them through any US bank or payment system. As a result of this law, most of the popular online casino networks such as Party Gaming and PlayTech left the United States. Overnight, online casino players found themselves being chased by the Federal government. But, after a fortnight, the online casino industry came up with a solution and new online casinos started taking root. These began to operate under a different business umbrella, and by doing that, rendered the transfer of money to and from them legal. A major part of this was enlisting electronic banking systems that would accept this new clarification and start doing business with me. Listed in this article are the electronic banking systems that accept players from the United States that wish to play in online casinos.</p>
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<li><em>Escherichia coli</em> (TOP 10)</li>
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<li><em>Agrobacterium tumefaciens</em> (GV3101)</li>
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<li><em>Alternaria alternata</em></li>
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</ul>
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<img src="img/a2.jpg" alt="" class="img-fluid float-left mr-20 mb-20">
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<p>In addition to develop our project, we need the following plant species:</p>
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<p>Recently, the US Federal government banned online casinos from operating in America by making it illegal to transfer money to them through any US bank or payment system. As a result of this law, most of the popular online casino networks such as Party Gaming and PlayTech left the United States. Overnight, online casino players found themselves being chased by the Federal government. But, after a fortnight, the online casino industry came up with a solution and new online casinos started taking root. These began to operate under a different business umbrella, and by doing that, rendered the transfer of money to and from them legal. A major part of this was enlisting electronic banking systems that would accept this new clarification and start doing business with me. Listed in this article are the electronic banking systems that accept players from the United States that wish to play in online casinos.</p>
+
<ul>
 +
<li><em>Nicotiana benthamiana</em></li>
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<li><em>Arabidopsis thaliana</em></li>
 +
</ul>
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<p>In conclusion, from the first moment the experts have supervised all the steps in the laboratory. They have a broad experience working whit these organism and they are totally familiar with all experimental procedures used in this project. The organisms that we are going to use in this project have not potential risks outside the lab.</p>
 +
<p>From the first moment all the biosafety rules have been fulfilled.</p>
 +
<p>All the protocols have been made according to good practices, according to the laws and complying with iGEM’s security rules and policies.</p>
 +
<p>For more information, you can visit both the website with igem regulations:<br><a href="https://2018.igem.org/Safety/Rules" target="_blank">https://2018.igem.org/Safety/Rules</<></p>
 +
<p>like the white list here: <a href="https://2018.igem.org/Safety/White_List" target="_blank">https://2018.igem.org/Safety/White_List</a>.</p>
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Revision as of 10:32, 5 October 2018

Safety

Working a team of eight people in a laboratory is a very nice experience, very enriching, fun and, above all, it must be very safe. Since the project was raised we were clear that we are a High School team and that the important thing is the protection. Therefore, decisions were made about the design of the project to reduce possible risks to the maximum, how to choose on-pathogenic microorganisms and use all the necessary protective material.

Our project is based on developing a method for the purification of recombinant proteins in plants, simple and profitable, which has been developed with the Carbohydrate Metabolism group (belonging to the CSIC) in its laboratories at the Institute of Agrobiotechnology.

The laboratory where it has been developed el proyecto, tiene un nivel de bioseguridad 1 (low risk). Además, los organismos con los que trabajamos no presentan riesgo potencial.

However, during the entire time we were in the laboratory, we met the following security measures:

  • Supervised work

    At all times we have been supervised by the people in charge of the laboratory, who have been in charge of supervising the actions carried out and guiding us in the appropriate knowledge and techniques.

    Before entering the laboratory, we attended mandatory lectures on Health and Biosafety in the laboratory, where they also explained the protocols and actions to follow in case of emergency.

  • Prevention and safety measures

    We receive lab coats (each person with their own gown), safety goggles, respirators and nitrile and / or latex gloves.

    We had our own workspace, dedicated to the development of our project; and that we always kept clean and tidy.

    To reduce any type of risk, when we worked, we disinfected the Ethanol work area before and after each use.

    The laboratory has a biological safety cabinet in which we managed the bacteria to avoid external contamination.

    Any waste that we produced was deposited in its corresponding container and processed in the regulatory manner.

    In addition, Ethidium Bromide has been replaced in the laboratory by Midori Green, a compound with many fewer risks than the previous one.

  • Organisms

    To choose well the design of the project depended to avoid many possible biological risks. That is why it was decided to work with non-pathogenic organisms, which have no potential risk outside the laboratory, neither for humans, animals or the environment.

    These organisms are:

    • Escherichia coli (TOP 10)
    • Agrobacterium tumefaciens (GV3101)
    • Alternaria alternata

    In addition to develop our project, we need the following plant species:

    • Nicotiana benthamiana
    • Arabidopsis thaliana

    In conclusion, from the first moment the experts have supervised all the steps in the laboratory. They have a broad experience working whit these organism and they are totally familiar with all experimental procedures used in this project. The organisms that we are going to use in this project have not potential risks outside the lab.

    From the first moment all the biosafety rules have been fulfilled.

    All the protocols have been made according to good practices, according to the laws and complying with iGEM’s security rules and policies.

    For more information, you can visit both the website with igem regulations:
    https://2018.igem.org/Safety/Rules

    like the white list here: https://2018.igem.org/Safety/White_List.