Before we started our experiments, all of our team members responsible for the wet-lab experiments received detailed and rigid training on biosafety and lab safety from Dai and Liu. The training included standard experimental procedures, usage of biosafety cabinets and normative disposal of experimental waste etc. During the first few weeks since we started our experiments, Dr.Liu stayed with us all the way in the lab to supervise our manipulations. He gave us guidance and enlightened us when necessary. During our time in lab, we kept thorough lab records, including protocols, consideration on error-prone steps and careful discussions etc. None of us conducted experiments on our own until it could pass a check on lab safety and experimental operations.

The chassis organisms used in our project is E. coli DH5alpha, which is classified into Risk Group 1 and Safety Level 1. Because of this, It does not cause disease in healthy adult humans and is allowed to be operated in open bench. Our genetically modified bacteria were cautiously operated and discarded and surly not released to the natural environment to prevent interference to the natural environment.

In order to minimize potentially risks that could be brought about by certain instruments and reagents, our members were requested to use normative protective equipment and follow the safety manuals throughout our lab time. Instruments like alcohol burner and centrifugal machine were carefully used according to the safety manuals. Gloves, lab coats, pants and close-toed shoes are demanded during lab time, and it is not allowed to touch about when wearing gloves. Besides, no food or drink is allowed in the working space.