Difference between revisions of "Team:NU Kazakhstan/Notebook"

Revision as of 07:33, 12 October 2018

Bioremediation of Sour Crude Oil Waste using Cyanobacteria

Subculture of Cyanobacteria

1. Resuspend and take 200μL of cyanobacteria from the old solution of BG11-media
2. Place into fresh 50 mL BG11-media
3. Leave it under continuous illumination and shaking on the shaker at room temperature in the hood
4. Check OD at 750nm

Preparation of BG11-media Stock solutions for BG-11:
A) Stock 1:

Filter sterilize into a sterile bottle or autoclave
Stock 2:

MgSO4 . 7H2O

7.5g/liter

Filter sterilize into a sterile bottle or autoclave
Stock 3:

K2HPO4 . 3H2O	4.0g/liter
or K2HPO4	3.05g/liter

Filter sterilize into a sterile bottle or autoclave
Stock 5 (Microelements):

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-				<h3 class="text-heading"><span>PROTOCOLS</span> <a href="#"><span style="border-bottom: 1px dashed #777; ">&nbsp; PROJECT TIMELINE</span></a></h3>
+				<h3 class="text-heading"><span>PROTOCOLS</span> <a href="https://2018.igem.org/Team:NU_Kazakhstan/Project_Timeline"><span style="border-bottom: 1px dashed #777; ">&nbsp; PROJECT TIMELINE</span></a></h3>
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