Difference between revisions of "Team:NU Kazakhstan/Notebook"
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| − | <h1 style="color: #fff; border-bottom: none; font-weight: bold!important"> | + | <h1 style="color: #fff; border-bottom: none; font-weight: bold!important">Overview & Background</h1> |
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| − | <h3 class="text-heading"><span>PROTOCOLS</span> <a href=" | + | <h3 class="text-heading"><span>PROTOCOLS</span> <a href="https://2018.igem.org/Team:NU_Kazakhstan/Project_Timeline"><span style="border-bottom: 1px dashed #777; "> PROJECT TIMELINE</span></a></h3> |
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<li>3. Leave it under continuous illumination and shaking on the shaker at room temperature in the hood</li> | <li>3. Leave it under continuous illumination and shaking on the shaker at room temperature in the hood</li> | ||
<li>4. Check OD at 750nm</li> | <li>4. Check OD at 750nm</li> | ||
| − | <ol> | + | </ol> |
<u><b>Preparation of BG11-media</b></u> | <u><b>Preparation of BG11-media</b></u> | ||
<b>Stock solutions for BG-11:<br> | <b>Stock solutions for BG-11:<br> | ||
| − | A) Stock 1:</b><br> | + | <b>A) Stock 1:</b><br> |
<table> | <table> | ||
<tr><td>Na2MG EDTA</td><td>0.1g/liter</td></tr> | <tr><td>Na2MG EDTA</td><td>0.1g/liter</td></tr> | ||
| − | tr><td>NFerric ammonium citrate</td><td>0.6g/liter</td></tr> | + | <tr><td>NFerric ammonium citrate</td><td>0.6g/liter</td></tr> |
| − | tr><td>NCitric acid . 1H2O</td><td>0.6g/liter</td></tr> | + | <tr><td>NCitric acid . 1H2O</td><td>0.6g/liter</td></tr> |
| − | tr><td>NCaCl2 . 2H2O</td><td>3.6g/liter</td></tr> | + | <tr><td>NCaCl2 . 2H2O</td><td>3.6g/liter</td></tr> |
</table> | </table> | ||
Filter sterilize into a sterile bottle or autoclave | Filter sterilize into a sterile bottle or autoclave | ||
| + | <br> | ||
| + | <b>Stock 2:</b><br> | ||
| + | <table> | ||
| + | <tr><td>MgSO4 . 7H2O</td><td>7.5g/liter</td></tr> | ||
| + | </table> | ||
| + | Filter sterilize into a sterile bottle or autoclave | ||
| + | <br> | ||
| + | S<b>tock 3:</b><br> | ||
| + | <table> | ||
| + | <tr><td>K2HPO4 . 3H2O</td><td>4.0g/liter</td></tr> | ||
| + | <tr><td>or K2HPO4</td><td>3.05g/liter</td></tr> | ||
| + | </table> | ||
| + | Filter sterilize into a sterile bottle or autoclave | ||
| + | <br> | ||
| + | |||
| + | <br><b>Stock 5 (Microelements):</b></br> | ||
| + | |||
| + | <table> | ||
| + | <tr><td>H3BO3</td><td>2.86g/liter</td></tr> | ||
| + | <tr><td>MnCl2 . 4H2O</td><td>1.81g/liter</td></tr> | ||
| + | <tr><td>ZnSO4 . 7H2O</td><td>0.222g/liter</td></tr> | ||
| + | <tr><td>CuSO4 . 5H2O</td><td>0.079g/liter</td></tr> | ||
| + | <tr><td>COCl2 . 6H2O</td><td>0.050g/liter</td></tr> | ||
| + | <tr><td>NaMoO4 . 2H2O</td><td>0.391g/liter</td></tr> | ||
| + | <tr><td>or MoO4 (85%)</td><td>0.018g/liter</td></tr> | ||
| + | </table> | ||
| + | |||
</div> | </div> | ||
</section> | </section> | ||
Latest revision as of 07:34, 12 October 2018
PROTOCOLS PROJECT TIMELINE
Subculture of Cyanobacteria
A) Stock 1:
Filter sterilize into a sterile bottle or autoclave
Stock 2:
Filter sterilize into a sterile bottle or autoclave
Stock 3:
Filter sterilize into a sterile bottle or autoclave
Stock 5 (Microelements):
- 1. Resuspend and take 200μL of cyanobacteria from the old solution of BG11-media
- 2. Place into fresh 50 mL BG11-media
- 3. Leave it under continuous illumination and shaking on the shaker at room temperature in the hood
- 4. Check OD at 750nm
A) Stock 1:
| Na2MG EDTA | 0.1g/liter |
| NFerric ammonium citrate | 0.6g/liter |
| NCitric acid . 1H2O | 0.6g/liter |
| NCaCl2 . 2H2O | 3.6g/liter |
Stock 2:
| MgSO4 . 7H2O | 7.5g/liter |
Stock 3:
| K2HPO4 . 3H2O | 4.0g/liter |
| or K2HPO4 | 3.05g/liter |
Stock 5 (Microelements):
| H3BO3 | 2.86g/liter |
| MnCl2 . 4H2O | 1.81g/liter |
| ZnSO4 . 7H2O | 0.222g/liter |
| CuSO4 . 5H2O | 0.079g/liter |
| COCl2 . 6H2O | 0.050g/liter |
| NaMoO4 . 2H2O | 0.391g/liter |
| or MoO4 (85%) | 0.018g/liter |