Difference between revisions of "Team:NU Kazakhstan/Notebook"
| Line 38: | Line 38: | ||
<u><b>Preparation of BG11-media</b></u> | <u><b>Preparation of BG11-media</b></u> | ||
<b>Stock solutions for BG-11:<br> | <b>Stock solutions for BG-11:<br> | ||
| − | A) Stock 1:</b><br> | + | <b>A) Stock 1:</b><br> |
<table> | <table> | ||
<tr><td>Na2MG EDTA</td><td>0.1g/liter</td></tr> | <tr><td>Na2MG EDTA</td><td>0.1g/liter</td></tr> | ||
| Line 47: | Line 47: | ||
Filter sterilize into a sterile bottle or autoclave | Filter sterilize into a sterile bottle or autoclave | ||
<br> | <br> | ||
| − | Stock 2:</b><br> | + | <b>Stock 2:</b><br> |
<table> | <table> | ||
<tr><td>MgSO4 . 7H2O</td><td>7.5g/liter</td></tr> | <tr><td>MgSO4 . 7H2O</td><td>7.5g/liter</td></tr> | ||
| Line 53: | Line 53: | ||
Filter sterilize into a sterile bottle or autoclave | Filter sterilize into a sterile bottle or autoclave | ||
<br> | <br> | ||
| − | | + | S<b>tock 3:</b><br> |
<table> | <table> | ||
<tr><td>K2HPO4 . 3H2O</td><td>4.0g/liter</td></tr> | <tr><td>K2HPO4 . 3H2O</td><td>4.0g/liter</td></tr> | ||
| Line 59: | Line 59: | ||
</table> | </table> | ||
Filter sterilize into a sterile bottle or autoclave | Filter sterilize into a sterile bottle or autoclave | ||
| − | + | <br> | |
<br><b>Stock 5 (Microelements):</b></br> | <br><b>Stock 5 (Microelements):</b></br> | ||
Latest revision as of 07:34, 12 October 2018
PROTOCOLS PROJECT TIMELINE
Subculture of Cyanobacteria
A) Stock 1:
Filter sterilize into a sterile bottle or autoclave
Stock 2:
Filter sterilize into a sterile bottle or autoclave
Stock 3:
Filter sterilize into a sterile bottle or autoclave
Stock 5 (Microelements):
- 1. Resuspend and take 200μL of cyanobacteria from the old solution of BG11-media
- 2. Place into fresh 50 mL BG11-media
- 3. Leave it under continuous illumination and shaking on the shaker at room temperature in the hood
- 4. Check OD at 750nm
A) Stock 1:
| Na2MG EDTA | 0.1g/liter |
| NFerric ammonium citrate | 0.6g/liter |
| NCitric acid . 1H2O | 0.6g/liter |
| NCaCl2 . 2H2O | 3.6g/liter |
Stock 2:
| MgSO4 . 7H2O | 7.5g/liter |
Stock 3:
| K2HPO4 . 3H2O | 4.0g/liter |
| or K2HPO4 | 3.05g/liter |
Stock 5 (Microelements):
| H3BO3 | 2.86g/liter |
| MnCl2 . 4H2O | 1.81g/liter |
| ZnSO4 . 7H2O | 0.222g/liter |
| CuSO4 . 5H2O | 0.079g/liter |
| COCl2 . 6H2O | 0.050g/liter |
| NaMoO4 . 2H2O | 0.391g/liter |
| or MoO4 (85%) | 0.018g/liter |