Difference between revisions of "Team:FJNU-China/Design"

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<link rel="stylesheet" type="text/css" href="https://2018.igem.org/Template:FJNU-China/CSS/bootstrap-min?action=raw&amp;ctype=text/css
 
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<a class="navbar-brand" href="https://2018.igem.org/Team:FJNU-China""><img src="https://static.igem.org/mediawiki/2018/9/9d/T--FJNU-China--index_logo.png"  width="100px" height="80px" alt="FJNU-China " style="margin-top: -5px !important;"></a>
<a class="navbar-brand" href="https://2018.igem.org/Team:FJNU-China""><img src="https://static.igem.org/mediawiki/2018/9/9d/T--FJNU-China--index_logo.png" alt="FJNU-China" style="margin-top: -5px !important;"></a></div>
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<ul class="nav navbar-nav main-menu">
 
<ul class="nav navbar-nav main-menu">
 
<li class="clearfix"><a href="https://2018.igem.org/Team:FJNU-China">Home</a></li>
 
<li class="clearfix"><a href="https://2018.igem.org/Team:FJNU-China">Home</a></li>
 
<li class="clearfix"><a href="#">Team</a>
 
<li class="clearfix"><a href="#">Team</a>
 
<ul class="dropdown-menu">
 
<ul class="dropdown-menu">
<li><a href="https://2018.igem.org/Team:FJNU-China/Team">About us</a></li>
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<li><a href="https://2018.igem.org/Team:FJNU-China/Team">About us</a></li>
<li><a href="https://2018.igem.org/Team:FJNU-China/Attributions">Attributions</a></li>
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<li><a href="https://2018.igem.org/Team:FJNU-China/Attributions">Attributions</a></li>
<li><a href="https://2018.igem.org/Team:FJNU-China/Gallery">Gallery</a></li>
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<li><a href="https://2018.igem.org/Team:FJNU-China/Gallery">Gallery</a></li>
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</ul>
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</li>
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<li class="active"><a href="#">Projects</a>
 
<li class="active"><a href="#">Projects</a>
 
<ul class="dropdown-menu">
 
<ul class="dropdown-menu">
<li><a href="https://2018.igem.org/Team:FJNU-China/Description">Description</a></li>
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<li><a href="https://2018.igem.org/Team:FJNU-China/Description">Description</a></li>
<li ><a href="https://2018.igem.org/Team:FJNU-China/Design">Design</a></li>
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<li ><a href="https://2018.igem.org/Team:FJNU-China/Design">Design</a></li>
<li ><a href="https://2018.igem.org/Team:FJNU-China/Result">Results</a></li>
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<li ><a href="https://2018.igem.org/Team:FJNU-China/Result">Results</a></li>
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<li><a href="https://2018.igem.org/Team:FJNU-China/Demonstrate">Demonstrate</a></li>
 
<li><a href="https://2018.igem.org/Team:FJNU-China/Demonstrate">Demonstrate</a></li>
 
<li><a href="https://2018.igem.org/Team:FJNU-China/Model">Model</a></li>
 
<li><a href="https://2018.igem.org/Team:FJNU-China/Model">Model</a></li>
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</ul>
 
</li>
 
</li>
 
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<li class="clearfix"><a href="#">Laboratory</a>
<li class="clearfix"><a href="#">Laboratory</a>
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<ul class="dropdown-menu">
 
<ul class="dropdown-menu">
 
<li><a href="https://2018.igem.org/Team:FJNU-China/Notebook">Notebook</a></li>
 
<li><a href="https://2018.igem.org/Team:FJNU-China/Notebook">Notebook</a></li>
<li><a href="https://2018.igem.org/Team:FJNU-China/InterLab">InterLab</a></li>
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<li><a href="https://2018.igem.org/Team:FJNU-China/InterLab">InterLab</a></li>
 
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<li><a href="https://2018.igem.org/Team:FJNU-China/Safety">Safety</a></li>
<li><a href="https://2018.igem.org/Team:FJNU-China/Safety">Safety</a></li>
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</ul>
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</li>
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<li class="clearfix"><a href="#">Parts</a>
<li class="clearfix"><a href="#">Parts</a>
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<ul class="dropdown-menu">
 
<ul class="dropdown-menu">
<li><a href="https://2018.igem.org/Team:FJNU-China/Basic_Part">Basic Part</a></li>
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<li><a href="https://2018.igem.org/Team:FJNU-China/Basic_Part">Basic Part</a></li>
<li><a href="https://2018.igem.org/Team:FJNU-China/Composite_Part">Composite Part</a></li>
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<li><a href="https://2018.igem.org/Team:FJNU-China/Composite_Part">Composite Part</a></li>
<li><a href="https://2018.igem.org/Team:FJNU-China/Improve">Improve</a></li>
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<li><a href="https://2018.igem.org/Team:FJNU-China/Improve">Improve</a></li>
<li><a href="https://2018.igem.org/Team:FJNU-China/Parts">Part List</a></li>
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<li><a href="https://2018.igem.org/Team:FJNU-China/Parts">Part List</a></li>
</ul>
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</li> <li class="clearfix"><a href="#">HP</a>
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<li class="clearfix"><a href="#">HP</a>
 
<ul class="dropdown-menu">
 
<ul class="dropdown-menu">
<li><a href="https://2018.igem.org/Team:FJNU-China/Overview">Overview</a></li>
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<li><a href="https://2018.igem.org/Team:FJNU-China/Overview">Overview</a></li>
                                                          <li><a href="https://2018.igem.org/Team:FJNU-China/Human_Practices">Intergrated HP</a></li>
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                                <li><a href="https://2018.igem.org/Team:FJNU-China/Human_Practices">Intergrated HP</a></li>
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<li><a href="https://2018.igem.org/Team:FJNU-China/Public_Engagement">Engagement&amp;<br>Public Education</a></li>
<li><a href="https://2018.igem.org/Team:FJNU-China/Public_Engagement">Engagement&amp;<br>Public Education</a></li>
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<li><a href="https://2018.igem.org/Team:FJNU-China/Collaborations">Collaborations</a></li>
 
<li><a href="https://2018.igem.org/Team:FJNU-China/Collaborations">Collaborations</a></li>
 
 
</ul>
 
</ul>
 
</li>
 
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<li class=""><a href="https://2018.igem.org/Team:FJNU-China/Medals">Medals</a></li>
 
<li class=""><a href="https://2018.igem.org/Team:FJNU-China/Medals">Medals</a></li>
  
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<div class="container">
 
<div class="container">
  <h1 style="text-align: center;margin-top: 100px; font-size:35px;">Design</h1>
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  <h1 style="text-align: center;margin-top: 100px; font-size:40px; padding-bottom:5px;">Design</h1>
 
     <div class="row"  style="background-color:rgba(243,243,243,0.4); font-size: 20px; padding: 0 20 0 20;width:75% !important; margin:0 auto !important;-moz-box-shadow: 2px 2px 10px #909090;
 
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   <ul class="lift" style="font-size: 16px;">
 
   <ul class="lift" style="font-size: 16px;">
     <li>Abstract</li>
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     <li>Overview</li>
     <li>PLA</li>
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     <li>handbook</li>
     <li>2-PE</li>
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     <li>Academic lecture</li>
     <li>Garbage can</li>
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     <li>“smell game”</li>
     <li>Bromidrosis</li>
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     <li>Public activity</li>
     <li>Biosafty</li>
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     <li>Communication</li>
    <li>Demonstrate</li>
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     <div class="t1 col-md-12 col-xs-12">
 
     <div class="t1 col-md-12 col-xs-12">
<h2 class="target" style="color: tomato;">Abstract</h2>
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<h2 class="target" style="color: tomato;padding-bottom:5px">Public engagement & education</h2>
 
<div >
 
<div >
   <p>As we know, the purpose of modeling is mainly to provide guidance and solve problems for the team experiment. So, before we designed the model, we sorted out some of the problems the team was facing. Based on these three problems,.Because The effect of PLA concentration on the quantity Staphylococcus epidermidis.Vestibulum quis quam ut magna consequat faucibus. Pellentesque eget nisi a mi suscipit tincidunt. Ut tempus dictum risus. Pellentesque viverra sagittis quam at mattis. Suspendisse potenti. Aliquam sit amet gravida nibh, facilisis gravida odio. Phasellus auctor velit at lacus blandit, commodo iaculis justo viverra. Etiam vitae est arcu.</p>
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   <p>&nbsp;&nbsp;&nbsp;&nbsp;As we mentioned in our background, there is a number of microorganism in our daily life. This year our team focuses on the microbe pollution in our laboratory, which may cause by abandoned pipettetips, waste of medium, diverse biological reagent and so on. And the stink releases by the garbage gives us intuitive feelings and bothered us.</br>
    <img src="https://static.igem.org/mediawiki/2018/b/bc/T--FJNU-China--pick.png" style="width: 50%">
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After we learnt about the characterization of PLA and 2-PE, we would like to give this problem a nice try.
  <img src="https://static.igem.org/mediawiki/2018/5/5b/T--FJNU-China--pingtu.jpg" style="width: 100%;">
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</p>
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   <div class="t2 col-md-12 col-xs-12">
 
   <div class="t2 col-md-12 col-xs-12">
<h2 class="target" style="color: tomato;">PLA</h2>
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<h2 class="target" style="color: tomato;">Garbage lid</h2>
 
<div class="col-md-12 col-xs-12">
 
<div class="col-md-12 col-xs-12">
   <p>As we know, the purpose of modeling is mainly to provide guidance and solve problems for the team experiment. So, before we designed the model, we sorted out some of the problems the team was facing. Based on these three problems,.Because The effect of PLA concentration on the quantity Staphylococcus epidermidis.Vestibulum quis quam ut magna consequat faucibus. Pellentesque eget nisi a mi suscipit tincidunt. Ut tempus dictum risus. Pellentesque viverra sagittis quam at mattis. Suspendisse potenti. Aliquam sit amet gravida nibh, facilisis gravida odio. Phasellus auctor velit at lacus blandit, commodo iaculis justo viverra. Etiam vitae est arcu.</p>
+
   <p>&nbsp;&nbsp;&nbsp;&nbsp;Non-standard experimental operation is common but serious somehow, which could cause infection of disease. The pointed part of used pipettetips and different biological reagent are breeding grounds for bacteria. As the laboratory is the place we went to the most frequently and the garbage usually is not properly processed, this problem needs attention.</p>
 +
<img class="img-responsive center-block" src="https://static.igem.org/mediawiki/2018/5/5c/T--FJNU-China--PLA_in_lid.png" style="width: 60%;"> 
 +
<img class="img-responsive center-block" src="https://static.igem.org/mediawiki/2018/4/46/T--FJNU-China--2-PE_in_lid.png" style="width: 60%;">
  
  <img src="https://static.igem.org/mediawiki/2018/8/84/T--FJNU-China--Description-room_temperature.gif" style="width:100%;">
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<p>We designed our engineered bacteria in a special container, a garbage lid, to deal with this situation. We put it in the laboratory baker, constitutive promoter will bring PLA and 2-PE into play, to inhibit the growth in the experimental garbage and release roselike aromatic at the same time.</br>
 +
Taking biosafety into account, we added toxin protein, mazf, as a suicide gene, linking with light-control promoter to prevent our engineered bacteria revealing to the outer environment than the garbage lid.
 +
</p>
 +
<img class="img-responsive center-block" src="https://static.igem.org/mediawiki/2018/e/ee/T--FJNU-China--Description-mazEF.png" style="width: 60%;">
 +
<p>The promoter could be induced at visible light. Once the bacteria revealed from the container, mazf will express to cleavage the mRNA. And this is the rreason that we use light-proof ingredient to 3D-printed our garbage lid. . (Details can be found on our <a href="https://2018.igem.org/Team:FJNU-China/Applied_Design">Application </a>page.)</br>
 +
Within the laboratory can be controlled, we demonstrated the efficiency of PLA sterilization. And we also got the most comfortable concentration of 2-PE according to the data of our human practice.(Details can be found on our <a href="https://2018.igem.org/Team:FJNU-China/Human_Practices">Human Practices</a> page.)</p>
 
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   <div class="t3 col-md-12 col-xs-12">
 
   <div class="t3 col-md-12 col-xs-12">
<h2 class="target"style="color: tomato;">2-PE</h2>
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<h2 class="target"style="color: tomato;"> Bromidrosis</h2>
     <div class="col-md-7 col-xs-7">
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     <div class="col-md-12 col-xs-12">
   <p>As we know, the purpose of modeling is mainly to provide guidance and solve problems for the team experiment. So, before we designed the model, we sorted out some of the problems the team was facing. Based on these three problems,.Because The effect of PLA concentration on the quantity Staphylococcus epidermidis.Vestibulum quis quam ut magna consequat faucibus. Pellentesque eget nisi a mi suscipit tincidunt. Ut tempus dictum risus. Pellentesque eget nisi a mi suscipit tincidunt. Ut tempus dictum risus. </p>
+
   <p>&nbsp;&nbsp;&nbsp;&nbsp;After came up with the idea to solve the outer environment, we found the human environment is also interesting. Aim at another stink caused by bacteria, the bromidrosis is also an unpleasant smell which bothers people around. Two new circuits are applying to the goal of controlling bromidrosis. </br>
 +
When people sweat, there is specific salt concentration and temperature on the skin. We choose this feature as a starting point, designing a logical gate to produce PLA. Every basic components serve respective functions. dsrA is a temperature promoter from E.coli, more active at 37 degree than 25 degree. The osmotically control promoter proU provides sensitivity to the salt concentration from 0M to 0.3M. And global-regulate protein HNS which could specifically bind to osmotically control promoter proU, we use it as a specific repressor to inhibit the promoter downstream. 
 +
</p>
 
     </div>
 
     </div>
      <div class="col-md-5 col-xs-5">
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<img class="img-responsive center-block" src="https://static.igem.org/mediawiki/2018/b/ba/T--FJNU-China--on_skin.gif" style="width: 60%;">
<img src="https://static.igem.org/mediawiki/2018/b/bc/T--FJNU-China--pick.png" style="width: 100%"><img src="https://static.igem.org/mediawiki/2018/b/bc/T--FJNU-China--pick.png" style="width: 100%">
+
<p>&nbsp;&nbsp;&nbsp;&nbsp;When the circuits on the skin, temperature reaches 37 degree, the repressor protein LacI will be expressed to inhibit PlacI, the H-NS won’t be revealed. Then, once people sweat, the Na+ and Cl- will induce proU, the logical gate became “on” state.</p>
  </div>
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<img class="img-responsive center-block" src="https://static.igem.org/mediawiki/2018/7/79/T--FJNU-China--room-temperature.gif" style="width: 60%;">
 
</div>
 
</div>
  
  
 
   <div class="t4 col-md-12 col-xs-12">
 
   <div class="t4 col-md-12 col-xs-12">
<h2 class="target" style="color: tomato;">Garbage can</h2>
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<h2 class="target" style="color: tomato;">The “smell game”</h2>
 
<div >
 
<div >
   <p>As we know, the purpose of modeling is mainly to provide guidance and solve problems for the team experiment. So, before we designed the model, we sorted out some of the problems the team was facing. Based on these three problems,.Because The effect of PLA concentration on the quantity Staphylococcus epidermidis.Vestibulum quis quam ut magna consequat faucibus. Pellentesque eget nisi a mi suscipit tincidunt. Ut tempus dictum risus. Pellentesque viverra sagittis quam at mattis. Suspendisse potenti. Aliquam sit amet gravida nibh, facilisis gravida odio. Phasellus auctor velit at lacus blandit, commodo iaculis justo viverra. Etiam vitae est arcu.</p>
+
   <p>To integrate public opinions and concerns into the scientific process is increasingly important as new biological inventions could influence the humanity as a whole, the environment and our coexistence with nature. In our project, we encountered a problem, it is not clear what the most favorable concentration of 2-PE. To seek answer and integrate people’s opinions into our project, we designed the “smell game” to get the public involved, which we prepared different concentration of 2-PE, 3M2H and some other substances to let people smell and knew about their feelings. From the feedback, we found the majority of people think 10mmol/L of 2-PE is the most pleasant, and which guided us to improve our experiment. (More details on our <a href="https://2018.igem.org/Team:FJNU-China/Human_Practices">Integrated HP</a> page)</p>
   <img src="https://static.igem.org/mediawiki/2018/5/5b/T--FJNU-China--pingtu.jpg" style="width: 100%;">
+
   <img class="img-responsive center-block" src="https://static.igem.org/mediawiki/2018/5/5b/T--FJNU-China--pingtu.jpg" style="width: 80%;">
 +
  <img class="img-responsive center-block" src="https://static.igem.org/mediawiki/2018/5/5b/T--FJNU-China--pingtu.jpg" style="width: 80%;">
 
  </div>
 
  </div>
 
</div>
 
</div>
  
 
   <div class="t5 col-md-12 col-xs-12">
 
   <div class="t5 col-md-12 col-xs-12">
<h2 class="target" style="color: tomato;">Bromidrosis</h2>
+
<h2 class="target" style="color: tomato;">Public Activity</h2>
 
<div>
 
<div>
   <p>As we know, the purpose of modeling is mainly to provide guidance and solve problems for the team experiment. So, before we designed the model, we sorted out some of the problems the team was facing. Based on these three problems,.Because The effect of PLA concentration on the quantity Staphylococcus epidermidis.Vestibulum quis quam ut magna consequat faucibus. Pellentesque eget nisi a mi suscipit tincidunt. Ut tempus dictum risus. Pellentesque viverra sagittis quam at mattis. Suspendisse potenti. Aliquam sit amet gravida nibh, facilisis gravida odio. Phasellus auctor velit at lacus blandit, commodo iaculis justo viverra. Etiam vitae est arcu.</p>
+
  <h3 style="text-align:left;font-weight:bold;">During the 110th anniversary of our school</h3>
 +
   <p>In 2017, we welcomed our school’s 110th anniversary, when tens of thousands of people from all over the world came to our school to celebrate 110th birthday of FJNU (Fujian Normal University) together. It was the most lively and biggest gathering in our school, and we seized the opportunity to hold a public activity, engaging the public in showing the possibilities of synthetic biology and getting more people to know about iGEM. We made some posters about previous excellent projects for exhibition and designed some interesting games to introduce iGEM and synthetic biology. Our activity finally turned out to be a great success, more people learned about what iGEM is and what we can do with synthetic biology.</p>
 +
 +
<img class="img-responsive center-block" src="https://static.igem.org/mediawiki/2018/3/3b/T--FJNU-China--Engagement-13.jpg" style="width: 80%;"> 
 +
<img class="img-responsive center-block" src="https://static.igem.org/mediawiki/2018/b/b7/T--FJNU-China--Engagement-14.jpg" style="width: 80%;"> </br>
  
   <img src="https://static.igem.org/mediawiki/2018/b/ba/T--FJNU-China--on_skin.gif" style="width: 80%;"> <img src="https://static.igem.org/mediawiki/2018/7/79/T--FJNU-China--room-temperature.gif" style="width: 80%;"> <img src="https://static.igem.org/mediawiki/2018/b/bc/T--FJNU-China--producing-mode.gif" style="width: 80%;">  <img src="https://static.igem.org/mediawiki/2018/2/29/T--FJNU-China--killing-mode.gif" style="width: 80%;">  
+
   <h3 style="text-align:left;font-weight:bold;">Calling for environmental protection</h3>
 +
  <p>In the process of conducting our project, we found that the unpleasant smell and environment of garbage can surrounding us is not paid much attention, but that does have an influence on people’s lives. We organized a public activity in cooperation with volunteer association, to popularize knowledge of waste sorting and appeal to the public to try their best to protect the environment, which we prepared intriguing games and propaganda posters.</p>
 +
   
 +
<div class="tz-gallery">
  
</div>
+
<div class="row">
  </div>
+
 
  <div class="t6 col-md-12 col-xs-12">
+
<div class="col-sm-6 col-md-4">
<h2 class="target" style="color: tomato;">Biosafty</h2>
+
<a class="lightbox" href="https://static.igem.org/mediawiki/2018/c/c5/T--FJNU-China--Engagement-15.jpg">
<div>
+
<img src="https://static.igem.org/mediawiki/2018/c/c5/T--FJNU-China--Engagement-15.jpg" alt="FJNU-China-img">
<h3 style="text-align:left;"><i class="fa fa-hand-o-right"   style=" align:center; color:tomato;" ></i>&nbsp;&nbsp;Background</h3>
+
</a>
  <p>mazEF is a toxin-antitoxin module located on the Escherichia coli chromosome and that of some other bacteria, including pathogens. mazF specifies for a stable toxin, MazF, and mazE specifies for a labile antitoxin, MazE, that antagonizes MazF. (Engelberg-Kulka, H., et al. 2005)MazF is a toxic nuclease arresting cell growth through the mechanism of RNA cleavage and that MazE inhibits the RNase activity of MazF by forming a complex.(Syed, M. A., et al. 2011).We used mazF as our killer to ensure biosafty. </p>
+
</div>
<div class="col-sm-12 col-md-12">
+
<div class="col-sm-6 col-md-4">
<img  style="width:99%"src="https://static.igem.org/mediawiki/2018/e/ee/T--FJNU-China--Description-mazEF.png" alt="mazEF" style="width;85%;">
+
<a class="lightbox" href="https://static.igem.org/mediawiki/2018/d/dc/T--FJNU-China--Engagement-16.jpg">
</div>
+
<img src="https://static.igem.org/mediawiki/2018/d/dc/T--FJNU-China--Engagement-16.jpg" alt="FJNU-China-img">
 +
</a>
 +
</div>
 +
<div class="col-sm-6 col-md-4">
 +
<a class="lightbox" href="https://static.igem.org/mediawiki/2018/7/7e/T--FJNU-China--Engagement-17.jpg">
 +
<img src="https://static.igem.org/mediawiki/2018/7/7e/T--FJNU-China--Engagement-17.jpg" alt="FJNU-China-img">
 +
</a>
 +
</div>
  
<h3 style="text-align:left;"><i class="fa fa-hand-o-right"  style=" align:center; color:tomato;" ></i>&nbsp;&nbsp;Design</h3>
 
  <p>According to different scenarios, we designed different promoters to combine with mazF. With the humanization design, the biosafty of our projects is guaranteed. </p>
 
  
<h3 style="text-align:left;">&nbsp;&nbsp;&nbsp;&nbsp;Bromidrosis</h3>
 
  <p>In our scent spray, we designed a suicide switch induced by arabinose. When we don't want the engineered bacteria to work again, we can kill the engineered bacteria by spraying a spray containing arabinose. In order to ensure that our engineering bacteria do not work in other environments, we set the switch of PLA and 2-PE to salt control and temperature control. Only when the temperature reaches the body temperature condition and the salinity reaches the human sweating condition, our engineering bacteria will play a role. </p>
 
<div class="col-sm-12 col-md-12">
 
<img  style="width:99%"src="https://static.igem.org/mediawiki/2018/e/ee/T--FJNU-China--Description-mazEF.png" alt="mazEF">
 
</div>
 
<h3 style="text-align:left;">&nbsp;&nbsp;&nbsp;&nbsp;Garbage can</h3>
 
  <p>In the trash can lid, we chose a light-controlled promoter to combine with mazF. Once the engineered bacteria leaves the dark environment of the trash can, it will be killed. </p>
 
<div class="col-sm-12 col-md-12">
 
<img  style="width:99%"src="https://static.igem.org/mediawiki/2018/e/ee/T--FJNU-China--Description-mazEF.png" alt="mazEF">
 
 
</div>
 
</div>
 +
  </div>
  
  
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   </div>
 
   </div>
  
 +
  <div class="t6 col-md-12 col-xs-12">
 +
<h2 class="target" style="color: tomato;">Events & Communication</h2>
 +
<div>
 +
  <h3 style="text-align:left;font-weight:bold;">the first iGEM Eurasian meetup 2018</h3>
 +
  <p>We participated in the first iGEM Eurasian meetup 2018 in Shenzhen, where we firstly presented our project on a more formal occasion. As the team representing our school to participate in iGEM for the first time, we constantly communicate with obvious experienced iGEMers and sought advice from experts during the conference. There had we also a collision of ideas with other teams.</p>
 +
 +
<img class="img-responsive center-block" src="https://static.igem.org/mediawiki/2018/1/11/T--FJNU-China--Engagement-18.jpg" style="width: 80%;"> 
 +
<img class="img-responsive center-block" src="https://static.igem.org/mediawiki/2018/4/45/T--FJNU-China--Engagement-19.jpg" style="width: 80%;"> </br>
  
  <div class="t7 col-md-12 col-xs-12">
+
   <h3 style="text-align:left;font-weight:bold;">the 5th CCiC</h3>
<h2 class="target" style="color: tomato;">Demonstrate</h2>
+
   <p>We took part in the 5th CCiC (Conference of China iGEMer Community) in Shanghai, which is the biggest gathering of iGEMers in China, aiming to promote the development of synthetic biology and improve the connectivity and communication of nationwide iGEM teams. On the conference, we made a preview and experienced the atmosphere of Giant Jamboree in advance. Also we established good friendship with other teams and got lots of useful advice to improve our project.</p>
<div >
+
<img class="img-responsive center-block" src="https://static.igem.org/mediawiki/2018/3/34/T--FJNU-China--Engagement-20.jpg" style="width: 80%;">   
   <h3 style="text-align: left;">EurAsian Meetup</h3>
+
<img class="img-responsive center-block" src="https://static.igem.org/mediawiki/2018/c/cf/T--FJNU-China--Engagement-21.png" style="width: 80%;">
  <img src="https://static.igem.org/mediawiki/2018/b/bc/T--FJNU-China--pick.png" style="width: 45%;"> <img src="https://static.igem.org/mediawiki/2018/b/bc/T--FJNU-China--pick.png" style="width: 45%;">
+
   
   <p>As we know, the purpose of modeling is mainly to provide guidance and solve problems for the team experiment. So, before we designed the model, we sorted out some of the problems the team was facing. Based on these three problems,.Because The effect of PLA concentration on the quantity Staphylococcus epidermidis.Vestibulum quis quam ut magna consequat faucibus. Pellentesque eget nisi a mi suscipit tincidunt. Ut tempus dictum risus. Pellentesque viverra sagittis quam at mattis. Suspendisse potenti. Aliquam sit amet gravida nibh, facilisis gravida odio. Phasellus auctor velit at lacus blandit, commodo iaculis justo viverra. Etiam vitae est arcu.</p>
+
  <h3 style="text-align:left;font-weight:bold;">Communicate with other teams</h3>
    <h3 style="text-align: left;">5th CCiC</h3>
+
   <p>It is of great importance to share experience and exchange ideas with others, which makes iGEM more wonderful and helps us find out our shortcomings. This year, we communicated and collaborated with many teams. (More details on our<a href="https://2018.igem.org/Team:FJNU-China/Collaborations"> Collaborations</a> page)</p>
  <img src="https://static.igem.org/mediawiki/2018/b/bc/T--FJNU-China--pick.png" style="width: 45%;">  <img src="https://static.igem.org/mediawiki/2018/b/bc/T--FJNU-China--pick.png" style="width: 45%;">   
+
 
   <p>As we know, the purpose of modeling is mainly to provide guidance and solve problems for the team experiment. So, before we designed the model, we sorted out some of the problems the team was facing. Based on these three problems,.Because The effect of PLA concentration on the quantity Staphylococcus epidermidis.Vestibulum quis quam ut magna consequat faucibus. Pellentesque eget nisi a mi suscipit tincidunt. Ut tempus dictum risus. Pellentesque viverra sagittis quam at mattis. Suspendisse potenti. Aliquam sit amet gravida nibh, facilisis gravida odio. Phasellus auctor velit at lacus blandit, commodo iaculis justo viverra. Etiam vitae est arcu.</p>
+
  
  
 
</div>
 
</div>
 
   </div>
 
   </div>
 +
 +
  
 
</div>
 
</div>
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     "control1": ".lift-nav",              //侧栏电梯的容器
 
     "control1": ".lift-nav",              //侧栏电梯的容器
 
     "control2": ".lift",                          //需要遍历的电梯的父元素
 
     "control2": ".lift",                          //需要遍历的电梯的父元素
     "target": [".t1",".t2",".t3",".t4",".t5",".t6",".t7"], //监听的内容,注意一定要从小到大输入
+
     "target": [".t1",".t2",".t3",".t4",".t5",".t6"], //监听的内容,注意一定要从小到大输入
 
     "current": "current"              //选中的样式
 
     "current": "current"              //选中的样式
 
   });
 
   });
 
})
 
})
 
</script>
 
</script>
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<script type="text/javascript" src="https://2018.igem.org/Template:FJNU-China/Javascript/box?action=raw&amp;ctype=text/javascript"></script>
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<script type="text/javascript">
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baguetteBox.run('.tz-gallery');
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</body>
 
</body>
 
</html>
 
</html>

Revision as of 21:09, 12 October 2018

Design

  • Overview
  • handbook
  • Academic lecture
  • “smell game”
  • Public activity
  • Communication

Public engagement & education

    As we mentioned in our background, there is a number of microorganism in our daily life. This year our team focuses on the microbe pollution in our laboratory, which may cause by abandoned pipettetips, waste of medium, diverse biological reagent and so on. And the stink releases by the garbage gives us intuitive feelings and bothered us.
After we learnt about the characterization of PLA and 2-PE, we would like to give this problem a nice try.

Garbage lid

    Non-standard experimental operation is common but serious somehow, which could cause infection of disease. The pointed part of used pipettetips and different biological reagent are breeding grounds for bacteria. As the laboratory is the place we went to the most frequently and the garbage usually is not properly processed, this problem needs attention.

We designed our engineered bacteria in a special container, a garbage lid, to deal with this situation. We put it in the laboratory baker, constitutive promoter will bring PLA and 2-PE into play, to inhibit the growth in the experimental garbage and release roselike aromatic at the same time.
Taking biosafety into account, we added toxin protein, mazf, as a suicide gene, linking with light-control promoter to prevent our engineered bacteria revealing to the outer environment than the garbage lid.

The promoter could be induced at visible light. Once the bacteria revealed from the container, mazf will express to cleavage the mRNA. And this is the rreason that we use light-proof ingredient to 3D-printed our garbage lid. . (Details can be found on our Application page.)
Within the laboratory can be controlled, we demonstrated the efficiency of PLA sterilization. And we also got the most comfortable concentration of 2-PE according to the data of our human practice.(Details can be found on our Human Practices page.)

Bromidrosis

    After came up with the idea to solve the outer environment, we found the human environment is also interesting. Aim at another stink caused by bacteria, the bromidrosis is also an unpleasant smell which bothers people around. Two new circuits are applying to the goal of controlling bromidrosis.
When people sweat, there is specific salt concentration and temperature on the skin. We choose this feature as a starting point, designing a logical gate to produce PLA. Every basic components serve respective functions. dsrA is a temperature promoter from E.coli, more active at 37 degree than 25 degree. The osmotically control promoter proU provides sensitivity to the salt concentration from 0M to 0.3M. And global-regulate protein HNS which could specifically bind to osmotically control promoter proU, we use it as a specific repressor to inhibit the promoter downstream.

    When the circuits on the skin, temperature reaches 37 degree, the repressor protein LacI will be expressed to inhibit PlacI, the H-NS won’t be revealed. Then, once people sweat, the Na+ and Cl- will induce proU, the logical gate became “on” state.

The “smell game”

To integrate public opinions and concerns into the scientific process is increasingly important as new biological inventions could influence the humanity as a whole, the environment and our coexistence with nature. In our project, we encountered a problem, it is not clear what the most favorable concentration of 2-PE. To seek answer and integrate people’s opinions into our project, we designed the “smell game” to get the public involved, which we prepared different concentration of 2-PE, 3M2H and some other substances to let people smell and knew about their feelings. From the feedback, we found the majority of people think 10mmol/L of 2-PE is the most pleasant, and which guided us to improve our experiment. (More details on our Integrated HP page)

Public Activity

During the 110th anniversary of our school

In 2017, we welcomed our school’s 110th anniversary, when tens of thousands of people from all over the world came to our school to celebrate 110th birthday of FJNU (Fujian Normal University) together. It was the most lively and biggest gathering in our school, and we seized the opportunity to hold a public activity, engaging the public in showing the possibilities of synthetic biology and getting more people to know about iGEM. We made some posters about previous excellent projects for exhibition and designed some interesting games to introduce iGEM and synthetic biology. Our activity finally turned out to be a great success, more people learned about what iGEM is and what we can do with synthetic biology.


Calling for environmental protection

In the process of conducting our project, we found that the unpleasant smell and environment of garbage can surrounding us is not paid much attention, but that does have an influence on people’s lives. We organized a public activity in cooperation with volunteer association, to popularize knowledge of waste sorting and appeal to the public to try their best to protect the environment, which we prepared intriguing games and propaganda posters.

Events & Communication

the first iGEM Eurasian meetup 2018

We participated in the first iGEM Eurasian meetup 2018 in Shenzhen, where we firstly presented our project on a more formal occasion. As the team representing our school to participate in iGEM for the first time, we constantly communicate with obvious experienced iGEMers and sought advice from experts during the conference. There had we also a collision of ideas with other teams.


the 5th CCiC

We took part in the 5th CCiC (Conference of China iGEMer Community) in Shanghai, which is the biggest gathering of iGEMers in China, aiming to promote the development of synthetic biology and improve the connectivity and communication of nationwide iGEM teams. On the conference, we made a preview and experienced the atmosphere of Giant Jamboree in advance. Also we established good friendship with other teams and got lots of useful advice to improve our project.

Communicate with other teams

It is of great importance to share experience and exchange ideas with others, which makes iGEM more wonderful and helps us find out our shortcomings. This year, we communicated and collaborated with many teams. (More details on our Collaborations page)