Difference between revisions of "Team:USP-Brazil/Results"

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             <p>After some trouble with gene synthesis and building our constructs, we got to doing experiments with the first three candidates for Sender systems (Lux, Las, Rhl) and all of the six Receiver systems. In this page, we will go through each one of the Sender systems and report how our reporters responded to the signal.
 
             <p>After some trouble with gene synthesis and building our constructs, we got to doing experiments with the first three candidates for Sender systems (Lux, Las, Rhl) and all of the six Receiver systems. In this page, we will go through each one of the Sender systems and report how our reporters responded to the signal.
 
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             </p>
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             <p>Our experiments were normally done in 15mL falcon tubes or 24-well plates (with admitted differences in aeration) and in 2 mL LB or M9 (for kinetic experiments) medium, at 37ºC and shaking at 180~200RPM. As the auto-induced quorum sensing system should be kickstarted by arabinose (see <a href="2018.igem.org/Team:USP-Brazil/Design">Design</a>), after growing an overnight culture diluted 1:10 for one hour, we induced our cells with 2.5mM L-arabinose and started measurements.
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             <p>Our experiments were normally done in 15mL falcon tubes or 24-well plates (with admitted differences in aeration) and in 2 mL LB or M9 (for kinetic experiments) medium, at 37ºC and shaking at 180~200RPM. As the auto-induced quorum sensing system should be kickstarted by arabinose (see <a href="/Design">Design</a>), after growing an overnight culture diluted 1:10 for one hour, we induced our cells with 2.5mM L-arabinose and started measurements.
 
             </p>
 
             </p>
 
           </div>
 
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Revision as of 21:53, 13 October 2018

Wiki - iGEM Brazil

Results

After some trouble with gene synthesis and building our constructs, we got to doing experiments with the first three candidates for Sender systems (Lux, Las, Rhl) and all of the six Receiver systems. In this page, we will go through each one of the Sender systems and report how our reporters responded to the signal.

Our experiments were normally done in 15mL falcon tubes or 24-well plates (with admitted differences in aeration) and in 2 mL LB or M9 (for kinetic experiments) medium, at 37ºC and shaking at 180~200RPM. As the auto-induced quorum sensing system should be kickstarted by arabinose (see Design), after growing an overnight culture diluted 1:10 for one hour, we induced our cells with 2.5mM L-arabinose and started measurements.