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− | With the use of site-direted mutagenesis we wanted to improve last years projects part. Removing the fusion protein and the unnecessary bases as shown in fig. 1. By doing this the fluorescent protein mNeonGreen (mNG) can be used as a reporter in a fully functional operone, with the use of the AraC/pBAD inducible system. | + | With the use of site-direted mutagenesis we wanted to improve the part of last years LiU iGEM team. This was done through removing the fusion protein and the unnecessary bases as shown in fig. 1. By doing this the fluorescent protein mNeonGreen (mNG) can be used as a reporter in a fully functional operone, with the use of the AraC/pBAD inducible system. |
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Revision as of 08:16, 14 October 2018
LiU iGEM
Improvement of Liu iGEM 2017s part
BBa_K2474000
With the use of site-direted mutagenesis we wanted to improve the part of last years LiU iGEM team. This was done through removing the fusion protein and the unnecessary bases as shown in fig. 1. By doing this the fluorescent protein mNeonGreen (mNG) can be used as a reporter in a fully functional operone, with the use of the AraC/pBAD inducible system.