Difference between revisions of "Team:SSHS-Shenzhen/Results"

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    <meta charset="UTF-8">
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    <title>Title</title>
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<div class="column full_size">
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<h1>Results</h1>
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Results
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<p>Here you can describe the results of your project and your future plans. </p>
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<h1>
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The result
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<p id="para">
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After finishing the vitro transcription, we put the adult P. striolata into glass bottles for doing the RNAi efficiency test.
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<h3>What should this page contain?</h3>
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<ul>
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<div class="ibox">
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<li> Clearly and objectively describe the results of your work.</li>
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<center><img src="https://static.igem.org/mediawiki/2018/c/c7/T--SSHS-Shenzhen--demo1.jpg" width="100%"></center>
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<li> Future plans for the project. </li>
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<p id="note">
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<li> Considerations for replicating the experiments. </li>
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<b>
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</ul>
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Fig.5-1
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</b>  
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Adult P. striolata and Brassica chinensis leaves were placed in the glass bottles. The solutions of siRNA/shRNA (10 ng/mL) were separately sprayed onto the leaves of Chinese cabbage every third day, each solution has two repeats. Around twenty adult beetles of P. striolata were tested per siRNA/shRNA sample.
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<center><img src="
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https://static.igem.org/mediawiki/2018/b/b7/T--SSHS-Shenzhen--results1.jpg
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<p id="note">
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Table 5-1
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<div class="ibox">
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<center><img src="
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https://static.igem.org/mediawiki/2018/5/58/T--SSHS-Shenzhen--results2.jpg
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<p id="note">
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Fig. 5-2
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The survival rate of Phyllotreta striolata at different days after siRNA/ shRNA treatment.
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<h3>Describe what your results mean </h3>
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<li> Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for. </li>
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https://static.igem.org/mediawiki/2018/d/db/T--SSHS-Shenzhen--results3-1.jpg
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<li> Show data, but remember all measurement and characterization data must be on part pages in the Registry. </li>
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" width="100%"></center>
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<li> Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project. </li>
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Fig. 5-3
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Comparison of RNAi efficiencies between siRNA and shRNA
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<h3> Project Achievements </h3>
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Fig. 5-4
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<p>You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.</p>
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Comparison of RNAi efficiencies between different shRNAs
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<ul>
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<li>A list of linked bullet points of the successful results during your project</li>
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<li>A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.</li>
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<div class="highlight decoration_A_full">
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https://static.igem.org/mediawiki/2018/1/13/T--SSHS-Shenzhen--results4.jpg
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<h3>Inspiration</h3>
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<p>See how other teams presented their results.</p>
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<p id="note">
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<ul>
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<b>
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<li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results/Pathway">2014 TU Darmstadt </a></li>
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Table 5-2
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<li><a href="https://2014.igem.org/Team:Imperial/Results">2014 Imperial </a></li>
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<li><a href="https://2014.igem.org/Team:Paris_Bettencourt/Results">2014 Paris Bettencourt </a></li>
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The effect of nucleotide content of shRNA on RNAi efficiency
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<p id="para">
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By comparing the different degrees of decreasing survival rates, between siRNA and shRNA (Fig. 5-2), and between different shRNAs (Fig. 5-3), the RNAi efficiency had been showed.
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Results show that all the samples tested, except ALR-siRNA-1, and AlR-shRNA-1, could trigger RNAi mechanism, which was demonstrated by the survival rate decrease after treatment (Table 5-1, Fig. 5-1). After 11 days of treatment, there was a slight decrease of survival rate in water treatment (100% to 94%), in ALR-siRNA-1 (100%-81%) and in ALR-shRNA-1(100%-100%). The survival rate decrease of other treatments are significant (between 34.5%-85%). The differences of RNAi efficiency between siRNA and its corresponding shRNA, which have the same target site, are not significant (Fig. 5-2). But the nucleotide content of siRNA/shRNA seems play a role in RNAi efficiency (Fig. 5-3). When the antisense strand of the siRNA/shRNA has a weak base pairing at 3′-end (presence of A/U), but a strong base pairing at 5′-end (presence of G/C), such as ALR-siRNA-1 and ALR-shRNA-1 (Table 5-3), the RNAi efficiency of this siRNA/shRNA is very low. This result may be caused by the failure of the antisense strand to be loaded into RNA-induced silencing complex (RISC), due to the lower free energy level at the 5’end comparing to that at the 3’end.
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</p>
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<br><br><br><br><br>
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</body>
 
</html>
 
</html>

Revision as of 12:40, 14 October 2018

Title

Title

Results

The result

After finishing the vitro transcription, we put the adult P. striolata into glass bottles for doing the RNAi efficiency test.

Fig.5-1 Adult P. striolata and Brassica chinensis leaves were placed in the glass bottles. The solutions of siRNA/shRNA (10 ng/mL) were separately sprayed onto the leaves of Chinese cabbage every third day, each solution has two repeats. Around twenty adult beetles of P. striolata were tested per siRNA/shRNA sample.

Table 5-1

Fig. 5-2 The survival rate of Phyllotreta striolata at different days after siRNA/ shRNA treatment.

Fig. 5-3 Comparison of RNAi efficiencies between siRNA and shRNA

Fig. 5-4 Comparison of RNAi efficiencies between different shRNAs

Table 5-2 The effect of nucleotide content of shRNA on RNAi efficiency

By comparing the different degrees of decreasing survival rates, between siRNA and shRNA (Fig. 5-2), and between different shRNAs (Fig. 5-3), the RNAi efficiency had been showed. Results show that all the samples tested, except ALR-siRNA-1, and AlR-shRNA-1, could trigger RNAi mechanism, which was demonstrated by the survival rate decrease after treatment (Table 5-1, Fig. 5-1). After 11 days of treatment, there was a slight decrease of survival rate in water treatment (100% to 94%), in ALR-siRNA-1 (100%-81%) and in ALR-shRNA-1(100%-100%). The survival rate decrease of other treatments are significant (between 34.5%-85%). The differences of RNAi efficiency between siRNA and its corresponding shRNA, which have the same target site, are not significant (Fig. 5-2). But the nucleotide content of siRNA/shRNA seems play a role in RNAi efficiency (Fig. 5-3). When the antisense strand of the siRNA/shRNA has a weak base pairing at 3′-end (presence of A/U), but a strong base pairing at 5′-end (presence of G/C), such as ALR-siRNA-1 and ALR-shRNA-1 (Table 5-3), the RNAi efficiency of this siRNA/shRNA is very low. This result may be caused by the failure of the antisense strand to be loaded into RNA-induced silencing complex (RISC), due to the lower free energy level at the 5’end comparing to that at the 3’end.