Difference between revisions of "Team:UMaryland/BCmodel"

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Our PET degradation system is entirely cell free, created from lysed cells that have had the pathway enzymes inserted into their genomes as plasmids. PET degradation starts with the cleavage of the monomers of PET into their constituents, MHET and TPA, by the enzyme PETase.<br><br>
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A 500mL PET water bottle from a common brand weight 4 grams, is 23 centimeters tall, and has a diameter of 7 cm. If we flatten this bottle out into a sheet, its surface area will be roughly 1000 square centimeters. This sheet will be covered by sheets of bacterial cellulose on either side.<br><br>
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We created cells that can act as a sensor for PET degradation. When the cells import PCA (a molecule produced from degraded TPA, which is produced from degraded PET), PCAU binds to PCA, creating activated PCAU. Using activated PCAU as the promoter for GFP translation, the cells will fluoresce green in the presence of PCA, showing that PET degradation has occurred.<br><br>
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All of the parameters of our model have been taken from experimentally verified results, and we have verified the validity of our model by successfully detecting PCA using our modified  cells.<br><br>
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We attempted to go further down the degradation pathway with the same cells metabolising PCA into 3-carboxy-cis,cis-muconate, but were unsuccessful. We leave this part of the pathway to future IGEM teams and researchers.<br>
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Explaination of our Parameters
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It's really long. Just download this <a href="https://static.igem.org/mediawiki/2018/e/e7/T--UMaryland--simbioparameters.xlsx"><u>Excel file</u></a> instead.
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Results of Simulation
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<img src="https://static.igem.org/mediawiki/2018/a/a9/T--UMaryland--modelresults.png" style="max-width: 100%" alt="Waluigi Time!">
 
<img src="https://static.igem.org/mediawiki/2018/a/a9/T--UMaryland--modelresults.png" style="max-width: 100%" alt="Waluigi Time!">
 
<div class="imageBoxDescription">Figure 1 - Results of the model</div>
 
<div class="imageBoxDescription">Figure 1 - Results of the model</div>
 
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Comparison to Experimental Results
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Revision as of 03:39, 16 October 2018

Template Title Template Title

Bacterial Cellulose Modeling
How fast can PETase CBD degrade a bottle with cellulose binding?
A 500mL PET water bottle from a common brand weight 4 grams, is 23 centimeters tall, and has a diameter of 7 cm. If we flatten this bottle out into a sheet, its surface area will be roughly 1000 square centimeters. This sheet will be covered by sheets of bacterial cellulose on either side.

Waluigi Time!
Figure 1 - Results of the model

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