Team:UMaryland/History

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History
Past iGEM Teams involved with PET
Listed here are past iGEM teams that have worked on projects on or related to PET degredation, with condensed descriptions of their results.
2012
BAU-Indonesia
  • Isolation of cutinase gene from nature with primers

TU Darmstadt
  • Surface display of cutinase on E. coli
  • Attempted TPA transport into E. coli, further research required
  • Expressed all TPH enzymes, did not attempt to measure activity
  • Confirmed anaerobic conversion of PCA via AroY and XylE enzymes

UC Davis
  • Confirmed cutinase activity using PNPB esterase assay
  • Engineered E. coli ethylene glycol metabolism with directed evolution

2013
Imperial College
  • Produced P3HB bioplastic from mixed waste containing at least some PET

2014
METU Turkey
  • Reduced catechol (downstream product) to pyruvate

ITB Indonesia
  • LC cutinatse activity confimed with SEM, PNPB

2015
Pasteur Paris
  • PNPB assay to confirm activity of esterase EST13
  • Fluorescent detection of TPA can not be accomplished when in LB broth

2016
ASIJ Tokyo
  • Attempt at detecting PET degradation by mass change failed

AUC Turkey
  • Withdrawn

Baltimore BioCrew
  • Planned to weigh PET degradation, no results

BGU Israel
  • PNPB and EM to confirm LC cutinase activity
  • P. putida can grow on PCA as sole carbon source, but not TPA
  • E. coli expressing LC-cutinase with pelB leader sequence grew on M9 plates with PET as sole carbon source. Expected to be due to consumption of ethylene glycol from PET degradation
  • Unable to determine enzyme efficiency based on growth due to heterogeneity in PET distribution
  • Measured fluorescence of TPA on plates, unable to quantify LC cutinase activity

Harvard BioDesign
  • Petase function confirmed with PNPB
  • D. tsuruhatensis produced electric current when supplied with unspecified quantity of TPA in M9 media

Tianjin
  • EM confirmation of PETase activity of PET film degradation
  • Multispectral scanning quantified PETase products for cell free system

TJUSLS China
  • HPLC detection of MHET to confirm PETase activity in varying conditions
  • Surface display of PETase in E. coli

UESTC-China
  • SEM and PNPB to confirm PETase activity
  • Possible detection of TPA by UV vis (higher absorbance across spectrum)

UoA New Zealand
  • Assembled PETase part with His tag

2017
Baltimore Bio-Crew
  • Fluorescine diacetate hydrolysis assay to confirm PETase and MHETase hydrolytic activity

BOKU-Vienna
    Discussion of a possible method for directed evolution of PETase by culturing cells on PET film that fluoresces when degraded

ITB Indonesia
  • Successful biofilm formation on PET, but biofilm matrix hampered PETase activity
  • Engineered E. coli ethylene glycol metabolism with directed evolution

2018
Makerere University
  • TBA

OLS Canmore Canada
  • TBA

RHIT
  • TBA

ULaVerne
  • TBA

UMaryland
  • Check out our results on this wiki!

Yale
  • TBA

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