Difference between revisions of "Team:SSHS-Shenzhen/Description"

 
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{{SSHS-Shenzhen/CSS}}
 
{{SSHS-Shenzhen/CSS}}
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<html lang="en">
 
<html lang="en">
 
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    <title>Title</title>
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.header li a:hover,.dropdown:hover.dropbtn {
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padding:30px 30px 5px;
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.header {
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font-size: 30px;
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left: 0;
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width: 100%;
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height: 3em;
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z-index: 99;
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float: left;
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background-color: #5d8aa8;
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min-width: 1080px;
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body {
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#fig{
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line-height: 500px;
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<div class="banner2">
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Phyllotreta striolata is the most destructive insects worldwide. However, the present insect control strategies have certain limitations. We aim to develop a RNAi-based topical application approach. Ingestion of the sprayed siRNAs or shRNAs by P. striolata will induce the RNA silencing mechanism in the P. striolata and lead to its death. So far, we have designed the siRNAs for synthesis based on the sequences of the genes that are important for the development and survival of P. striolata. Currently we are rearing P. striolata to obtain enough the same age insects for feeding bioassays and gene silencing investigation. We will design corresponding small hairpin RNAs (shRNAs) and use E. coli expression system, or in vitro transcription system to produce these shRNAs in large scale.
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Abstract
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<h1>
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Abstract
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Phyllotreta striolata is one of the most destructive insects worldwide. However, the present insect control strategies have certain limitations. Here, we aim to develop a RNAi-based approach for controlling P. striolata. This approach is to topically apply exogenous siRNAs/shRNAs onto vegetables, ingestion of the sprayed siRNAs or shRNAs by P. striolata will induce the RNAi mechanism in the insect and lead to its death. In our project, siRNAs/shRNAs were designed based on the mRNA sequences of their target genes. The efficiency of both siRNAs and shRNAs in mediating RNAi in P. striolata were examined. Experimental results show that both siRNAs and shRNA could successfully silence their target genes, which was demonstrated by the survival rate decrease after siRNA or shRNA treatment. Our project provided an environmentally friendly approach for pest control.</p>
  
  
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Latest revision as of 11:58, 16 October 2018

Title

title
Abstract

Abstract

Phyllotreta striolata is one of the most destructive insects worldwide. However, the present insect control strategies have certain limitations. Here, we aim to develop a RNAi-based approach for controlling P. striolata. This approach is to topically apply exogenous siRNAs/shRNAs onto vegetables, ingestion of the sprayed siRNAs or shRNAs by P. striolata will induce the RNAi mechanism in the insect and lead to its death. In our project, siRNAs/shRNAs were designed based on the mRNA sequences of their target genes. The efficiency of both siRNAs and shRNAs in mediating RNAi in P. striolata were examined. Experimental results show that both siRNAs and shRNA could successfully silence their target genes, which was demonstrated by the survival rate decrease after siRNA or shRNA treatment. Our project provided an environmentally friendly approach for pest control.