Difference between revisions of "Team:Edinburgh UG/Demonstrate"

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               <h1 class="brand-heading">Demonstrate</h1>
 
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               <p class="intro-text">All aspects of our project combine to provide a novel chassis for Synthetic Biology: Maxicells. These maxicells are easy to use and can be safely deployed into the environment through the use of our Triple Lock System.</p>
 
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              <h2 style="text-align:left">Maxicell Characterisation</h2>
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              <ul><li style="text-align:left">We have assessed various methods of maxicell production and have concluded that they are easy to make through UV exposure and homing endonuclease action</li>
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                  <li style="text-align:left">Our knockdown construct <a href="http://parts.igem.org/Part:BBa_K2725008">BBa_K2725008</a> allows maxicell production by theoretically any strain of <i>E. coli</i></li>
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                  <li style="text-align:left">We have shown that maxicells can function as a biosensor</li>
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                  <li style="text-align:left">The active lifespan of maxicells was found to be enough for real-world applications: over 18 hours</li>
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              <h2 style="text-align:left">The Triple Lock System</h2>
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              <ul><li style="text-align:left">We have developed a model that allows fine-tuning of the maxicell lifespan through the use of a Colicin E2 kill switch</li>
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                  <li style="text-align:left">Semantic containment of our plasmid DNA was demonstrated as a feasible method of preventing expression of our genes by foreign organisms</li>
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                  <li style="text-align:left">Triclosan was successfully used as a selection marker as an alternative to antibiotic resistance</li>
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            <p style="text-align:left">When put together, our final maxicell product would therefore have virtually no risk to the biosphere upon environmental release.</p>
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Revision as of 14:05, 16 October 2018

Edinburgh iGEM 2018

Demonstrate

All aspects of our project combine to provide a novel chassis for Synthetic Biology: Maxicells. These maxicells are easy to use and can be safely deployed into the environment through the use of our Triple Lock System.

Maxicell Characterisation

  • We have assessed various methods of maxicell production and have concluded that they are easy to make through UV exposure and homing endonuclease action
  • Our knockdown construct BBa_K2725008 allows maxicell production by theoretically any strain of E. coli
  • We have shown that maxicells can function as a biosensor
  • The active lifespan of maxicells was found to be enough for real-world applications: over 18 hours

The Triple Lock System

  • We have developed a model that allows fine-tuning of the maxicell lifespan through the use of a Colicin E2 kill switch
  • Semantic containment of our plasmid DNA was demonstrated as a feasible method of preventing expression of our genes by foreign organisms
  • Triclosan was successfully used as a selection marker as an alternative to antibiotic resistance

When put together, our final maxicell product would therefore have virtually no risk to the biosphere upon environmental release.

Contact EdiGEM18

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