Difference between revisions of "Team:Munich/deletioncasette.html"
ArianeKrus (Talk | contribs) |
Franziwinzig (Talk | contribs) |
||
| Line 18: | Line 18: | ||
<tr> | <tr> | ||
<td>Notes:</td> | <td>Notes:</td> | ||
| − | <td> | + | <td>We also inoculated LB media blindly.</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href="https://2018.igem.org/Team:Munich/Results" target="_blank">Results:</a></td> | <td><a href="https://2018.igem.org/Team:Munich/Results" target="_blank">Results:</a></td> | ||
| − | <td> | + | <td>No colonies, no growth; <br> |
We decided to use a resistance cassette from pSB1C3 without FRT sites | We decided to use a resistance cassette from pSB1C3 without FRT sites | ||
</td> | </td> | ||
| Line 50: | Line 50: | ||
<tr> | <tr> | ||
<td><a href="https://2018.igem.org/Team:Munich/Results" target="_blank">Results:</a></td> | <td><a href="https://2018.igem.org/Team:Munich/Results" target="_blank">Results:</a></td> | ||
| − | <td>PIC. | + | <td>PIC. We thought the band might be what we wanted but because of how few DNA the extraction yielded, these samples were not used in further experiments. |
</td> | </td> | ||
</tr> | </tr> | ||
Revision as of 19:17, 16 October 2018
Transforming E. Coli DH5a to amplify pKD3 for pRED/ET engineering
2018/05/28| Participants: | Dominic Schwarz |
| Protocol: | Electrocompetent transformation |
| Notes: | We also inoculated LB media blindly. |
| Results: | No colonies, no growth; We decided to use a resistance cassette from pSB1C3 without FRT sites |
Amplifying a selection cassette from pSB1C3
2018/05/29| Participants: | Enikö Baligács |
| Protocol: | PCR, Agarose gel, Gel extraction |
| Notes: | Primer with 50bp homology regions ????, TA: 67°C, Elongation: 45s |
| Results: | PIC. We thought the band might be what we wanted but because of how few DNA the extraction yielded, these samples were not used in further experiments. |