Difference between revisions of "Team:Munich/assembly3.html"
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<td>Protocol:</td> | <td>Protocol:</td> | ||
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| − | <a href="https://static.igem.org/mediawiki/2018/c/c1/T--Munich--PhageT7AssemblyTXTL.pdf" target="_blank"> | + | <a href="https://static.igem.org/mediawiki/2018/c/c1/T--Munich--PhageT7AssemblyTXTL.pdf" target="_blank">Phage T7 Assembly TX-TL</a>, |
<a href="https://static.igem.org/mediawiki/2018/2/23/T--Munich--AgarOverlayPlaqueAssay_.pdf" target="_blank">Agar overlay plaque assay</a> | <a href="https://static.igem.org/mediawiki/2018/2/23/T--Munich--AgarOverlayPlaqueAssay_.pdf" target="_blank">Agar overlay plaque assay</a> | ||
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<td>Protocol:</td> | <td>Protocol:</td> | ||
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| − | <a href="https://static.igem.org/mediawiki/2018/c/c1/T--Munich--PhageT7AssemblyTXTL.pdf" target="_blank"> | + | <a href="https://static.igem.org/mediawiki/2018/c/c1/T--Munich--PhageT7AssemblyTXTL.pdf" target="_blank">Phage T7 Assembly TX-TL</a>, |
<a href="https://static.igem.org/mediawiki/2018/2/23/T--Munich--AgarOverlayPlaqueAssay_.pdf" target="_blank">Agar overlay plaque assay</a> | <a href="https://static.igem.org/mediawiki/2018/2/23/T--Munich--AgarOverlayPlaqueAssay_.pdf" target="_blank">Agar overlay plaque assay</a> | ||
</td> | </td> | ||
Revision as of 20:21, 16 October 2018
T7 and 3S Phage Assembly in self-produced cell-extract
2018/09/21- 2018/09/22| Participants: | Brigit Tunaj, Sophie v. Schönberg |
| Protocol: | Phage T7 Assembly TX-TL, Agar overlay plaque assay |
| Notes: | T7 DNA: ca. 500 ng, 3S Phage: 330 ng Self-produced extract: E10 |
| Results: | T7 Phage: 1,6*10^8 PFU/mL 3S: did not work out |
T7 Phage Assembly in self-produced cell-extract
2018/09/21- 2018/09/22| Participants: | Brigit Tunaj, Sophie v. Schönberg |
| Protocol: | Phage T7 Assembly TX-TL, Agar overlay plaque assay |
| Notes: | T7 DNA: ca. 500 ng, |
| Results: | T7 Phage in E10 Extract: 3*10^4 PFU/mL T7 Phage in A8 Extract: 2*10^4 PFU/mL |