Difference between revisions of "Team:JMU Wuerzburg/Results"

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            <h3>Wet Lab</h3>
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            <div>
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                In general, wWe can detect <span style="font-style: italic;">Plasmodia</span> in general in all five cultured <span style="font-style: italic;">Plasmodia</span> stems of
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                <span style="font-style: italic;">Plasmodium</span> falciparum (FCR3, Mali2K, HB3, Dd2 and 3D7 from Tuebingen and Heidelberg).
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                In addition, we can detect our Biobrick (BBa_K2614000) that includes a
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                synthetic template as a positive control for the qPCR that detects <span style="font-style: italic;">Plasmodium</span> in general (figure 1).
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                We can detect less than 1000 copies of our synthetic template with a probe (FAM)
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                and less than 50 copies with SybrGreen.
  
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                <div style="border-style: solid; border-color: #CCC; border-width: 1px; margin: 2em auto; width: 80%;">
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<h1>Results</h1>
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                    <img style="width: 100%;" src="src/pics/project-results_fig1.png" alt="fehlt" style="display: block;">  
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<p>Here you can describe the results of your project and your future plans. </p>
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                    <span style="text-decoration: underline;">Figure 1:</span> qPCR with cultured <span style="font-style: italic;">Plasmodia</span> stems and our BioBrick as a control for <span style="font-style: italic;">Plasmodium</span> general
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                </div>
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<h3>What should this page contain?</h3>
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<ul>
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<li> Clearly and objectively describe the results of your work.</li>
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<li> Future plans for the project. </li>
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<li> Considerations for replicating the experiments. </li>
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<h3>Describe what your results mean </h3>
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<ul>
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<li> Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for. </li>
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<li> Show data, but remember all measurement and characterization data must be on part pages in the Registry. </li>
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<li> Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project. </li>
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<h3> Project Achievements </h3>
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<p>You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.</p>
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<ul>
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<li>A list of linked bullet points of the successful results during your project</li>
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<li>A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.</li>
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</ul>
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                Moreover, we are able to detect P.
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                falciparum in all five cultured <span style="font-style: italic;">Plasmodia</span> stems.
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                Our BioBrick is a negative control for our qPCR that detects P.
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                falciparum.
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                With the probe (HEX) we detect less than ten copies of our synthetic template.
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                (figure 2)
  
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                <div style="border-style: solid; border-color: #CCC; border-width: 1px; margin: 2em auto; width: 80%;">
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                    <img style="width: 100%;" src="src/pics/project-results_fig2.png" alt="fehlt" style="display: block;">
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                    <span style="text-decoration: underline;">Figure 2:</span> qPCR with cultured <span style="font-style: italic;">Plasmodia</span> stems and our biobrick as a control for <span style="font-style: italic;">P. falciparum</span>.
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                </div>
  
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                In a multiplex system the two probes (FAM and HEX) also detect the five cultured <span style="font-style: italic;">Plasmodium</span> stems.
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                They detect them with nearly the same cq-mean which means that both qPCRs can detect <span style="font-style: italic;">Plasmodium</span>.
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                With this result we showed that multiplexing is also possible with the two primer/probe pairs (figure 3).
  
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<div class="highlight decoration_A_full">
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                    <img style="width: 100%;" src="src/pics/project-results_fig3.png" alt="fehlt" style="display: block;">  
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<h3>Inspiration</h3>
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                    <span style="text-decoration: underline;">Figure 3:</span> Multiplex qPCR with cultured <span style="font-style: italic;">Plasmodia</span> stems and our biobrick as a control for <span style="font-style: italic;">Plasmodium</span> general and specific for <span style="font-style: italic;">P. falciparum</span>
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<p>See how other teams presented their results.</p>
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                </div>
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<ul>
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<li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results/Pathway">2014 TU Darmstadt </a></li>
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<li><a href="https://2014.igem.org/Team:Imperial/Results">2014 Imperial </a></li>
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<li><a href="https://2014.igem.org/Team:Paris_Bettencourt/Results">2014 Paris Bettencourt </a></li>
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            <h3>Human practices</h3>
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            <div>
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                We organised different workshops from scientists about malaria or primer/probe design which were open for all interested students. There was also a pipetting test where more experienced students could give their knowledge to pipetting beginners.
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                <br><br>
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                At our “cake and science” events we presented our project to the public.
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            </div>
  
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            <h3>Interlab</h3>
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            <div>
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                We participated successfully at the <span style="font-style: italic;">fifth International InterLaboratory Measurement Study</span> in synthetic biology. At the iGEM 2018 Vibrigens InterLab Study from Marburg we also take place.
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            </div>
  
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            <h3>Collaboration</h3>
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            <div>
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                We were able to establish a very pleasant and close collaboration with team Munich. They have mentored us with great care and reliability and answered all our questions. So, it was much easier to start a new iGEM team in Wuerzburg. We also received advice from different members of team Tuebingen who were so kind to exchange some valuable information and experience with us.
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Revision as of 00:16, 17 October 2018

Wet Lab

In general, wWe can detect Plasmodia in general in all five cultured Plasmodia stems of Plasmodium falciparum (FCR3, Mali2K, HB3, Dd2 and 3D7 from Tuebingen and Heidelberg). In addition, we can detect our Biobrick (BBa_K2614000) that includes a synthetic template as a positive control for the qPCR that detects Plasmodium in general (figure 1). We can detect less than 1000 copies of our synthetic template with a probe (FAM) and less than 50 copies with SybrGreen.
fehlt Figure 1: qPCR with cultured Plasmodia stems and our BioBrick as a control for Plasmodium general
Moreover, we are able to detect P. falciparum in all five cultured Plasmodia stems. Our BioBrick is a negative control for our qPCR that detects P. falciparum. With the probe (HEX) we detect less than ten copies of our synthetic template. (figure 2)
fehlt Figure 2: qPCR with cultured Plasmodia stems and our biobrick as a control for P. falciparum.
In a multiplex system the two probes (FAM and HEX) also detect the five cultured Plasmodium stems. They detect them with nearly the same cq-mean which means that both qPCRs can detect Plasmodium. With this result we showed that multiplexing is also possible with the two primer/probe pairs (figure 3).
fehlt Figure 3: Multiplex qPCR with cultured Plasmodia stems and our biobrick as a control for Plasmodium general and specific for P. falciparum

Human practices

We organised different workshops from scientists about malaria or primer/probe design which were open for all interested students. There was also a pipetting test where more experienced students could give their knowledge to pipetting beginners.

At our “cake and science” events we presented our project to the public.

Interlab

We participated successfully at the fifth International InterLaboratory Measurement Study in synthetic biology. At the iGEM 2018 Vibrigens InterLab Study from Marburg we also take place.

Collaboration

We were able to establish a very pleasant and close collaboration with team Munich. They have mentored us with great care and reliability and answered all our questions. So, it was much easier to start a new iGEM team in Wuerzburg. We also received advice from different members of team Tuebingen who were so kind to exchange some valuable information and experience with us.