Difference between revisions of "Team:JMU Wuerzburg/Results"
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<span style="text-decoration: underline;">Figure 1:</span> qPCR with cultured <span style="font-style: italic;">Plasmodia</span> stems and our BioBrick as a control for <span style="font-style: italic;">Plasmodium</span> general | <span style="text-decoration: underline;">Figure 1:</span> qPCR with cultured <span style="font-style: italic;">Plasmodia</span> stems and our BioBrick as a control for <span style="font-style: italic;">Plasmodium</span> general | ||
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<span style="text-decoration: underline;">Figure 2:</span> qPCR with cultured <span style="font-style: italic;">Plasmodia</span> stems and our biobrick as a control for <span style="font-style: italic;">P. falciparum</span>. | <span style="text-decoration: underline;">Figure 2:</span> qPCR with cultured <span style="font-style: italic;">Plasmodia</span> stems and our biobrick as a control for <span style="font-style: italic;">P. falciparum</span>. | ||
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<span style="text-decoration: underline;">Figure 3:</span> Multiplex qPCR with cultured <span style="font-style: italic;">Plasmodia</span> stems and our biobrick as a control for <span style="font-style: italic;">Plasmodium</span> general and specific for <span style="font-style: italic;">P. falciparum</span> | <span style="text-decoration: underline;">Figure 3:</span> Multiplex qPCR with cultured <span style="font-style: italic;">Plasmodia</span> stems and our biobrick as a control for <span style="font-style: italic;">Plasmodium</span> general and specific for <span style="font-style: italic;">P. falciparum</span> | ||
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Revision as of 01:13, 17 October 2018
Wet Lab
In general, wWe can detect Plasmodia in general in all five cultured Plasmodia stems of
Plasmodium falciparum (FCR3, Mali2K, HB3, Dd2 and 3D7 from Tuebingen and Heidelberg).
In addition, we can detect our Biobrick (BBa_K2614000) that includes a
synthetic template as a positive control for the qPCR that detects Plasmodium in general (figure 1).
We can detect less than 1000 copies of our synthetic template with a probe (FAM)
and less than 50 copies with SybrGreen.
Figure 1: qPCR with cultured Plasmodia stems and our BioBrick as a control for Plasmodium general
Moreover, we are able to detect P.
falciparum in all five cultured Plasmodia stems.
Our BioBrick is a negative control for our qPCR that detects P.
falciparum.
With the probe (HEX) we detect less than ten copies of our synthetic template.
(figure 2)
Figure 2: qPCR with cultured Plasmodia stems and our biobrick as a control for P. falciparum.
In a multiplex system the two probes (FAM and HEX) also detect the five cultured Plasmodium stems.
They detect them with nearly the same cq-mean which means that both qPCRs can detect Plasmodium.
With this result we showed that multiplexing is also possible with the two primer/probe pairs (figure 3).
Figure 3: Multiplex qPCR with cultured Plasmodia stems and our biobrick as a control for Plasmodium general and specific for P. falciparum
Figure 1: qPCR with cultured Plasmodia stems and our BioBrick as a control for Plasmodium general
Figure 2: qPCR with cultured Plasmodia stems and our biobrick as a control for P. falciparum.
Figure 3: Multiplex qPCR with cultured Plasmodia stems and our biobrick as a control for Plasmodium general and specific for P. falciparum
Human practices
We organised different workshops from scientists about malaria or primer/probe design which were open for all interested students. There was also a pipetting test where more experienced students could give their knowledge to pipetting beginners.
At our “cake and science” events we presented our project to the public.
At our “cake and science” events we presented our project to the public.
Interlab
We participated successfully at the fifth International InterLaboratory Measurement Study in synthetic biology. At the iGEM 2018 Vibrigens InterLab Study from Marburg we also take place.
Collaboration
We were able to establish a very pleasant and close collaboration with team Munich. They have mentored us with great care and reliability and answered all our questions. So, it was much easier to start a new iGEM team in Wuerzburg. We also received advice from different members of team Tuebingen who were so kind to exchange some valuable information and experience with us.