Difference between revisions of "Team:JMU Wuerzburg/Basic Part"

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             <h3>Part BBa_K2614000</h3>
 
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                     <span style="text-decoration: underline;"></span> qPCR with primer/probe set for Plasmodium in general. BBa_K2614000 as positive control, cultured Plasmodium stems and negative control are shown.
 
                     <span style="text-decoration: underline;"></span> qPCR with primer/probe set for Plasmodium in general. BBa_K2614000 as positive control, cultured Plasmodium stems and negative control are shown.
 
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                     <span style="text-decoration: underline;"></span> Multiplex qPCR with primer/probe set for Plasmodium in general and Plasmodium falciparum. BBa_K2614000 as positive control, cultured Plasmodium stems and negative control are shown.
 
                     <span style="text-decoration: underline;"></span> Multiplex qPCR with primer/probe set for Plasmodium in general and Plasmodium falciparum. BBa_K2614000 as positive control, cultured Plasmodium stems and negative control are shown.
 
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Revision as of 01:14, 17 October 2018

Part BBa_K2614000

We developed a primer/probe set for a qPCR, which detects the human pathogens of the genus Plasmodium. This part can be used as positive control for our qPCR. If you like to run this qPCR you need one sense primer (CTGGCTAAACTTCCCAATGA), one antisense primer (GTCTATATTCATGTTTGATTGACCTT) and a dual labeled Probe ([6FAM]CTTcCAaATaGAtTTCGCAGAA[BHQ1] (lowercase letter = LNA)).
fehlt qPCR with primer/probe set for Plasmodium in general. BBa_K2614000 as positive control, cultured Plasmodium stems and negative control are shown.
fehlt Multiplex qPCR with primer/probe set for Plasmodium in general and Plasmodium falciparum. BBa_K2614000 as positive control, cultured Plasmodium stems and negative control are shown.