Project introduction：

Tetracycline is a kind of antibiotic substance separated from the culture solution of Streptomyces aureofa-ciens, which belong to the common broad-spectrum antibiotic and have a great effect on many types of microorganism, including gram-negative bacteria, gram-positive bacteria, rickettsia, filterable virus, spirochaeta and protozoa.

Recently, serious environment problems caused by Pharmaceuticals and Personal Care Products (PPCPs) massive abusing have attracted extensive attention in the world. Tetracycline antibiotics are the most frequently detected contamination in the PPCPs materials. As a kind of broad-spectrum antibiotic, all of the tetracycline antibiotic structures contain a naphthacene basic skeleton, including tetracycline, oxytetracycline, aureomycin and some other semi-synthetic derivatives. Tetracycline antibiotics are widely used worldwide because of their low price, especially in China, most of the comsumption would appear in the livestock industry, what is more, the number of the consumption and sale in this class of antibiotics would in the lead. The improper use of tetracycline would cause endogenous permanently discolored teeth, which usually called tetracycline stained teeth. Tetracycline antibiotics are often difficult to completely absorbed by the intestines of animals, and then almost 30% to 90% of those would be excreted as the parent compound form into the environment.

It is our key target to detect and degradate the tetracycline antibiotics in the nuatural environmet, including hydrographic basin, farm water or in soil. For that to happen, we have constructed two main working plasmid, detecting device and degradation device, which can achieve that sensitive detecting and then degradating the tetracycline immediately. In our project, we plan to use double ezymes system to degradate the tetracycline together, those are liginin peroxidases and manganese perioxidases. Finally, we want to develop a special tiny box to prevent our bacterial escaping from our working place and then improve the detecting and dagradating efficiency.