Difference between revisions of "Team:Aix-Marseille/Composite Part"
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<!-- Pour chaque part il serait bien d'expliquer d'ou vien la sequence (les parts utilisé pour le faire), ce que c'est censé faire (pourquoi on la construit), comment on a testé ce que ca fait essentiellement copier coller du registry --> | <!-- Pour chaque part il serait bien d'expliquer d'ou vien la sequence (les parts utilisé pour le faire), ce que c'est censé faire (pourquoi on la construit), comment on a testé ce que ca fait essentiellement copier coller du registry --> | ||
=BBa_K2718006= | =BBa_K2718006= | ||
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| + | Methionine gama lyase (MGL) catalises l-methionine in methanthiol, and with natural oxidation methanethiol begins DMTS ( Dimethyl trisulfide) and DMDS (Dimethyl disulfide). [http://parts.igem.org/Part:BBa_K2718022 BBa_K2718005] is in RFC 10, and part comes from [http://parts.igem.org/Part:BBa_K1493300 BBa_K1493300].To test activity we used DTNB and MTBH to mesure activity. DTNB react with thiol group and MTBH with 2-oxobutanoate [https://www.dovepress.com/activities-of-methionine-gamma-lyase-in-the-acidophilic-archaeon-ldquo-peer-reviewed-article-RRB]. | ||
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| + | https://static.igem.org/mediawiki/parts/1/16/--Aix-Marseille--Shcema_MGL_parts.jpg | ||
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=BBa_K2718010= | =BBa_K2718010= | ||
=BBa_K2718011= | =BBa_K2718011= | ||
=BBa_K2718022= | =BBa_K2718022= | ||
Revision as of 13:31, 17 October 2018
Composite Parts
Composite Parts
A composite part is a functional unit of DNA consisting of two or more basic parts assembled together. BBa_I13507 is an example of a composite part, consisting of an RBS, a protein coding region for a red fluorescent protein, and a terminator.
BBa_K2718006
Methionine gama lyase (MGL) catalises l-methionine in methanthiol, and with natural oxidation methanethiol begins DMTS ( Dimethyl trisulfide) and DMDS (Dimethyl disulfide). BBa_K2718005 is in RFC 10, and part comes from BBa_K1493300.To test activity we used DTNB and MTBH to mesure activity. DTNB react with thiol group and MTBH with 2-oxobutanoate [1].
