Difference between revisions of "Team:Munich/chibiobrick1.html"
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<td>Protocol:</td> | <td>Protocol:</td> | ||
<td><a href="https://www.sigmaaldrich.com/technical-documents/protocols/biology/annealing-oligos.html" target="_blank">Oligodimerization</a> | <td><a href="https://www.sigmaaldrich.com/technical-documents/protocols/biology/annealing-oligos.html" target="_blank">Oligodimerization</a> | ||
| + | </td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>Notes:</td> | ||
| + | <td> Oligodimers were bought | ||
</td> | </td> | ||
| + | </tr> | ||
| + | |||
</tr> | </tr> | ||
</table> | </table> | ||
Revision as of 14:40, 17 October 2018
Forming oligodimers from single-strand DNA chi6 sequences
2018/06/01| Participants: | Dominic Schwarz |
| Protocol: | Oligodimerization |
| Notes: | Oligodimers were bought |
Assembling pSB1C3_Chi6 with A3 Assembly
2018/06/04| Participants: | Dominic Schwarz |
| Protocol: | Restriction digest, Ligation, Chemical transformation, Miniprep, Sequencing |
| Notes: | Digest with XbaI, SpeI Transformation of Dh5a |
| Results: | No sequence obtained for the given primers. The samples must be contaminated. We decided to redo the experiment. |
Redo: Assembling pSB1C3_Chi6 with A3 Assembly
2018/06/06 - 2018/06/26| Participants: | Dominic Schwarz |
| Protocol: | Restriction digest, PCR purification, Ligation, Chemical Transformation, PCR, Agarose gel |
| Notes: | Digest with XbaI, SpeI Transformation of Dh5a |
| Results: | No bands on the Gel. Colonies on the plate resulted from contamination (religation of backbound). |