Difference between revisions of "Team:HFLS ZhejiangUnited"

 
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            <span dir="auto">Team:Worldshaper-XSHS</span>
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                     <a href="https://2018.igem.org/Team:HFLS_ZhejiangUnited" >
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                         worldshaper- <b>XSHS</b>
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                         HFLS_ZhejiangUnited
 
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                             <a href="https://2017.igem.org/Team:Worldshaper-XSHS">Home</a>
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                             <a href="https://2018.igem.org/Team:HFLS_ZhejiangUnited">Home</a>
 
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                                 Team
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                                         <a href="https://2017.igem.org/Team:Worldshaper-XSHS/aboutus.html">about us</a>
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                                         <a href="https://2018.igem.org/Team:HFLS_ZhejiangUnited/Description">Description</a>
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                                    </li>
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                                        <a href="https://2018.igem.org/Team:HFLS_ZhejiangUnited/Background">Background</a>
 
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                                         <a href="https://2018.igem.org/Team:HFLS_ZhejiangUnited/Applied_Design">Applied Design</a>
 
                                     </li>
 
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                                 Experiments
 
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                                         <a href="https://2018.igem.org/Team:HFLS_ZhejiangUnited/Results">Results</a>
 
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                                         <a href="https://2018.igem.org/Team:HFLS_ZhejiangUnited/Future_Work">Future Work</a>
 
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                                         <a href="https://2018.igem.org/Team:HFLS_ZhejiangUnited/Improve">Improved</a>
 
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                                         <a href="https://2018.igem.org/Team:HFLS_ZhejiangUnited/Protocol">Protocol</a>
 
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                                         <a href="https://2018.igem.org/Team:HFLS_ZhejiangUnited/Safety">Safety</a>
 
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                                        <a href="https://2017.igem.org/Team:Worldshaper-XSHS/Attributions">Attributions</a>
 
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                         <h2>Abstract</h2>
 
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                          <h3>Formaldehyde Eliminator: Engineered Microbes for Detecting and Biodegrading of Formaldehyde</h3>
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                          Hometown Hangzhou is widely known by water- related UNESCO World Heritages – West Lake and Grand Canal, however, severe water pollution problem caused by many reasons also exist. Hence, we hope to provide an easy-operating and low-cost tool for public to monitor water quality around.
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                            Formaldehyde brings different degrees of harmful symptoms to us humans, such as eye, throat and skin irritation, and even carcinogenicity, which is widespread used in construction and decoration industries. In previous iGEM projects related to formaldehyde, several problems still need to improved, such as 1) the present sensing threshold of formaldehyde concentration (~ 10 ppm) is far upper beyond the environment-protecting standard (~ 0.1 ppm); 2) the degrading system seems to work unstably, although some survival or duration after formaldehyde addition was observed. We constructed a more sensitive and effective E. coli-based system for detecting and further degrading formaldehyde in environments, basing on current systems. Additionally, to promote the real-world application, we tried to design a prototype which can be used in a real environment.
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                         <p>As following is the visual demonstration of our project. </p>
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                            We designed a portable water quality bio-detector prototype based on E.coli strains which were constructed to detect dissolved oxygen, phosphorus or nitrogen in water respectively. The oxygen sensitive vgb promoter and a GFP reporter constitute Oxygen detector 1.0. To enhance the expression of GFP, version 2.0 contains a vgb promoter, a T7 RNA polymerase gene, a T7 promoter and a GFP gene. For nitrogen, the PyeaR promoter was used to response to different concentrations of nitrate, nitrite and nitric, with a BFP reporter gene. For phosphate, a plasmid consisting of an ‘external phosphate sensing promoter’ to sense the phosphate concentration and a RFP gene to report.  
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                         <p>Worldshaper-XSHS, Xiaoshan High School</p>
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                         <p>HFLS Zhejiang United </p>
 
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                            Adress: No.538,Gongxiu Road,Xiaoshan District,Hangzhou,Zhejiang Province,China
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                        Schools: Peking University Experimental School(Jiaxing), Hangzhou Foreign Language School, etc.
 
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Latest revision as of 15:55, 17 October 2018

Abstract

Formaldehyde Eliminator: Engineered Microbes for Detecting and Biodegrading of Formaldehyde

Formaldehyde brings different degrees of harmful symptoms to us humans, such as eye, throat and skin irritation, and even carcinogenicity, which is widespread used in construction and decoration industries. In previous iGEM projects related to formaldehyde, several problems still need to improved, such as 1) the present sensing threshold of formaldehyde concentration (~ 10 ppm) is far upper beyond the environment-protecting standard (~ 0.1 ppm); 2) the degrading system seems to work unstably, although some survival or duration after formaldehyde addition was observed. We constructed a more sensitive and effective E. coli-based system for detecting and further degrading formaldehyde in environments, basing on current systems. Additionally, to promote the real-world application, we tried to design a prototype which can be used in a real environment.

As following is the visual demonstration of our project.

  • HFLS Zhejiang United

    Schools: Peking University Experimental School(Jiaxing), Hangzhou Foreign Language School, etc.