Difference between revisions of "Team:FJNU-China/Composite Part"

 
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  <br><span>&nbsp;&nbsp;&nbsp;&nbsp;This year we have designed five basic part.<a href="href="http://parts.igem.org/Part:BBa_K2570011"> BBa_K2570011 </a>is our favorite basic part. More information can be accessed by clicking on each of the following Part numbers.</span>
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       <caption style="color: white;font-size: 40px;margin:30px 0px !important; font-weight:bold !important;">Composite Parts</caption>
 
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<p style="margin-left: 57px;margin-right: 57px;">&nbsp;&nbsp;&nbsp;&nbsp;In our project, we combined temperature promoter dsrABBa_K2570015 and salt-concentration promoter proUBBa_K2467001 as a logical gate controlled by salt-concentration and temperature to realize the aim of producing antibacterial, PLA. dsrABBa_K2570015 is a temperature control promoter. </br>
 
&nbsp;&nbsp;&nbsp;&nbsp;The temperature range of dsrA is from 25 degree to 42 degree. With temperature increase, the promote efficiency is getting higher.<span style="vertical-align:super;"> [1]</span>The salt-concentration promoter proUBBa_K2467001 provides sensitivity to the salt concentration to human skin. proU is a promoter existed in E.coli, induced by low salt concentration. And the H-NSBBa_K2570016 is a global-regulated protein which could bind to genome and specifically inhibit proU.<span style="vertical-align:super;">[2]</span>
 
 
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<img src="https://static.igem.org/mediawiki/2017/8/8b/ED_OG_Interlab_Fig1.png"  style="display:block; margin:0 auto;padding-bottom: 1em;">
 
<p style="margin-left: 57px;margin-right: 57px;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;This year the iGEM Edinburgh_OG team improved the BBa_K191006 part to fulfil the gold medal criteria.A core principle of the Biobrick foundation is to bring standardisation in a field that is constantly changing and evolving. Standardising biological parts and their assembly allows for projects to focus less on assembly of genetic constructs and more on design and testing.
 
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[1] Temperature Sensing by the dsrA Promoter. JOURNAL OF BACTERIOLOGY, Nov. 2003, p. 6609–6614 DOI: 10.1128/JB.185.22.6609–6614.2003</br>
 
 
[2] RNase III initiates rapid degradation of proU mRNA upon hypo-osmotic stress in Escherichia coli. RNA Biology
 
  
  
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Latest revision as of 16:04, 17 October 2018

Parts


    This year we have designed five basic part. BBa_K2570011 is our favorite basic part. More information can be accessed by clicking on each of the following Part numbers.
Composite Parts
Part Number Type Part Description Length(bp)
BBa_K2570011 Composite dsrA+lacI+PlacI
+hns+ProU+GFP
In our project, we combined temperature promoter dsrA and salt-concentration promoter ProU as a logical gate controlled by salt-concentration and temperature to realize the aim of producing antibacterial, PLA. 3400
BBa_K2570009 Composite araC+atoD+atoA+adhE2 This is a composite part of degrading main unpleasant axilla odors E-3M2H(E-3-methyl-3-hexenoic acid). 5139
BBa_K2570010 Composite Aro10+PAR+TyrB Production of 2-PE 4184
BBa_K2570017 Composite dsrA+lacI+PlacI+hns
+ProU+TyrB+LDH+rocG
The promoter induced by LacI will express global regulate protein HNS, which could repress ProU. To solve promoter leak problem, our membrane provided a salt-free environment to prevent the production of PLA. 6964
BBa_K2570018 Composite TyrB+LDH+rocG Production of PLA. 3599