Difference between revisions of "Team:UofGuelph/Description"

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{{UofGuelph}}
 
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<h1>Description</h1>
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<p>Tell us about your project, describe what moves you and why this is something important for your team.</p>
 
  
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<h3>What should this page contain?</h3>
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<li> A clear and concise description of your project.</li>
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<li>A detailed explanation of why your team chose to work on this particular project.</li>
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<h3>Inspiration</h3>
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<p>See how other teams have described and presented their projects: </p>
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<h1 class="descHead">THIS IS THE 2017 TEMPLATE PAGE, NEED TO UPDATE</h1>
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<h1 class="descHead">Project Description</h1>
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<img src="https://static.igem.org/mediawiki/2017/4/4a/T--U_of_Guelph--gryphon.jpg" class="guelphImages">
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<h1 class="descSub">Beerstone, FRC and OXC</h1>
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<p class="descP">
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Calcium oxalate build up (commonly referred to as Beerstone) is a major ongoing problem in the brewing industry. Beerstone is difficult to remove from brewing equipment, requiring the use of harsh cleaning chemicals, and provides a porous, nutrient rich growing environment for bacterial biofilms. Our project is to develop a biological, enzyme based cleaning method for the removal of Beerstone from brewing equipment. We propose to use the enzymes FRC and OXC from <i> Oxalobacter formigenes </i> oxalate degrading metabolic pathway to breakdown Beerstone. </p>
  
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<ul>
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<h1 class="descSub">Project Overview</h1>
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<li><a href="https://2016.igem.org/Team:Imperial_College/Description">2016 Imperial College</a></li>
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<li><a href="https://2016.igem.org/Team:Wageningen_UR/Description">2016 Wageningen UR</a></li>
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<b>Step 1: Cloning of <i>frc</i> and <i>oxc</i> into DH5&alpha;</b> <br>
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<li><a href="https://2014.igem.org/Team:UC_Davis/Project_Overview"> 2014 UC Davis</a></li>
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-Synthesize <i>frc</i> and <i>oxc</i><br>
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<li><a href="https://2014.igem.org/Team:SYSU-Software/Overview">2014 SYSU Software</a></li>
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-Add PstI cut site to pET-28a <br>
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-Ligate <i>frc</i> and <i>oxc</i> into pET-28a <br>
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-Transform pET-28a<i>frc</i>/<i>oxc</i> into DH5&alpha; <br><br>
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<b>Step 2: Clone <i>frc</i> and <i>oxc</i> into BL21</b><br>
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-Purify pET-28a<i>frc</i>/<i>oxc</i> from DH5&alpha;<br>
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-Transform pET-28a<i>frc</i>/<i>oxc</i> into BL21<br><br>
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<b>Step 3: Express and Purify FRC and OXC</b><br>
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-Induce expression with IPTG and extract crude proteins<br>
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-Purify proteins using Ni-NTA chromatography<br><br>
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<b>Step 4: Characterize FRC and OXC</b><br>
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-Characterize enzyme function using Sodium Oxalate<br>
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-Characterize enzyme function using Calcium Oxalate<br><br>
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<b>Step 5: Design a Cleaning Solution and Test on Beerstone</b><br>
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-Test ability of enzymes to break down Beerstone<br>
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-Design a functional cleaning solution<br>
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</p>
  
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<h1 class="descSub">The 2017 Aspect</h1>
  
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<p class="descRef">
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Due to setbacks and unforeseen circumstances our team was only able to accomplish Step 1 this year. We will however be continuing our project next year where we left off. This winter we will be re-grouping in an effort to better plan for next year so that our project execution occurs more smoothly and we are able to accomplish more.
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<h3>Advice on writing your Project Description</h3>
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<a href="http://www.uoguelph.ca/" style="text-decoration: none;">
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We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be concise, accurate, and unambiguous in your achievements.  
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<a href="https://www.froggabio.com/" style="text-decoration: none;">
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<a href="https://www.neb.ca/" style="text-decoration: none;">
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<img class="footerlogo" src="https://static.igem.org/mediawiki/2017/c/cb/T--U_of_Guelph--InstagramLogo.png">
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<p class="footertext" style="text-align:center!important;">University of Guelph iGEM 2017</p>
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<h3>References</h3>
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<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you thought about your project and what works inspired you.</p>
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Revision as of 17:09, 17 October 2018

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THIS IS THE 2017 TEMPLATE PAGE, NEED TO UPDATE

Project Description

Beerstone, FRC and OXC

Calcium oxalate build up (commonly referred to as Beerstone) is a major ongoing problem in the brewing industry. Beerstone is difficult to remove from brewing equipment, requiring the use of harsh cleaning chemicals, and provides a porous, nutrient rich growing environment for bacterial biofilms. Our project is to develop a biological, enzyme based cleaning method for the removal of Beerstone from brewing equipment. We propose to use the enzymes FRC and OXC from Oxalobacter formigenes oxalate degrading metabolic pathway to breakdown Beerstone.

Project Overview

Step 1: Cloning of frc and oxc into DH5α
-Synthesize frc and oxc
-Add PstI cut site to pET-28a
-Ligate frc and oxc into pET-28a
-Transform pET-28afrc/oxc into DH5α

Step 2: Clone frc and oxc into BL21
-Purify pET-28afrc/oxc from DH5α
-Transform pET-28afrc/oxc into BL21

Step 3: Express and Purify FRC and OXC
-Induce expression with IPTG and extract crude proteins
-Purify proteins using Ni-NTA chromatography

Step 4: Characterize FRC and OXC
-Characterize enzyme function using Sodium Oxalate
-Characterize enzyme function using Calcium Oxalate

Step 5: Design a Cleaning Solution and Test on Beerstone
-Test ability of enzymes to break down Beerstone
-Design a functional cleaning solution

The 2017 Aspect

Due to setbacks and unforeseen circumstances our team was only able to accomplish Step 1 this year. We will however be continuing our project next year where we left off. This winter we will be re-grouping in an effort to better plan for next year so that our project execution occurs more smoothly and we are able to accomplish more.

University of Guelph iGEM 2017