Difference between revisions of "Team:Aix-Marseille/Results"
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==Pheromones production== | ==Pheromones production== | ||
| − | The aim was to produce two types of pheromones: DMTS/DMDS and benzaldehyde/benzylalcohol. We did some research on the two molecules to get the biosynthesis pathways to produce them in ''E. coli''. Firslty, we needed to add the three enzymes implicated in the biosynthesis of benzaldehyde/benzylalcohol: MdlB, MdlC and Hmas. We successfully cloned the Hmas ([http://parts.igem.org/wiki/index.php?title=Part:BBa_K2718011 BBa_K2718011]) | + | The aim was to produce two types of pheromones: DMTS/DMDS and benzaldehyde/benzylalcohol. We did some research on the two molecules to get the biosynthesis pathways to produce them in ''E. coli''. Firslty, we needed to add the three enzymes implicated in the biosynthesis of benzaldehyde/benzylalcohol: MdlB, MdlC and Hmas. We successfully cloned the Hmas gene ([http://parts.igem.org/wiki/index.php?title=Part:BBa_K2718011 BBa_K2718011]) and produced the equivalent protein. This enabled the production of the first metabolic intermediate (S-Mandelate) of the pathway. Secondly, we were able to clone the methionine-γ-lyase ([http://parts.igem.org/wiki/index.php?title=Part:BBa_K2718006 BBa_K2718006]) gene and produce the equivalent protein. This enable the achievement of a first goal: the production of the DMTS/DMDS pheromone. |
==Results of trap tests== | ==Results of trap tests== | ||
'''''(put results of trap tests on bed bugs)''''' | '''''(put results of trap tests on bed bugs)''''' | ||
Revision as of 17:18, 17 October 2018
Results
Wetlab
Beauveria bassiana
Pheromones production
The aim was to produce two types of pheromones: DMTS/DMDS and benzaldehyde/benzylalcohol. We did some research on the two molecules to get the biosynthesis pathways to produce them in E. coli. Firslty, we needed to add the three enzymes implicated in the biosynthesis of benzaldehyde/benzylalcohol: MdlB, MdlC and Hmas. We successfully cloned the Hmas gene (BBa_K2718011) and produced the equivalent protein. This enabled the production of the first metabolic intermediate (S-Mandelate) of the pathway. Secondly, we were able to clone the methionine-γ-lyase (BBa_K2718006) gene and produce the equivalent protein. This enable the achievement of a first goal: the production of the DMTS/DMDS pheromone.
Results of trap tests
(put results of trap tests on bed bugs)