Difference between revisions of "Team:Aix-Marseille/Results"
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{{Aix-Marseille|title=Results}} | {{Aix-Marseille|title=Results}} | ||
=Wetlab= | =Wetlab= | ||
| − | ==''Beauveria bassiana''= | + | ==Endochitinase== |
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| + | The endochitinase is a native protein produced by ''Beauveria bassiana'' an enthomopathogenic fungus. We wanted to overproduce (in ''E. coli'') and extract it to enhance the killing efficieny and speed of the fungus. We succesfully produced, purified a functional endochitinase (|[http://parts.igem.org/wiki/index.php?title=Part:BBa_K2718022 BBa_K2718022]) | ||
==Pheromones production== | ==Pheromones production== | ||
Revision as of 17:34, 17 October 2018
Results
Wetlab
Endochitinase
The endochitinase is a native protein produced by Beauveria bassiana an enthomopathogenic fungus. We wanted to overproduce (in E. coli) and extract it to enhance the killing efficieny and speed of the fungus. We succesfully produced, purified a functional endochitinase (|BBa_K2718022)
Pheromones production
The aim was to produce two types of pheromones: DMTS/DMDS and benzaldehyde/benzylalcohol. We did some research on the two molecules to get the biosynthesis pathways to produce them in E. coli. Firslty, we needed to add the three enzymes implicated in the biosynthesis of benzaldehyde/benzylalcohol: MdlB, MdlC and Hmas. We successfully cloned the Hmas gene (BBa_K2718011) and produced the equivalent protein. This enabled the production of the first metabolic intermediate (S-Mandelate) of the pathway. Secondly, we were able to clone the methionine-γ-lyase gene (BBa_K2718006) and produce the equivalent protein. This enable the achievement of a first goal: the production of the DMTS/DMDS pheromone.
Results of trap tests
(put results of trap tests on bed bugs)