Difference between revisions of "Team:Chalmers-Gothenburg/Protocols"

 
(8 intermediate revisions by 2 users not shown)
Line 55: Line 55:
 
     <li class="nav-item dropdown">
 
     <li class="nav-item dropdown">
 
       <a class="nav-link dropdown-toggle" id="navbardrop-project" role="button" data-toggle="dropdown">
 
       <a class="nav-link dropdown-toggle" id="navbardrop-project" role="button" data-toggle="dropdown">
−
         PROJECT OVERVIEW
+
         PROJECT
 
       </a>
 
       </a>
 
       <div class="dropdown-menu">
 
       <div class="dropdown-menu">
−
         <a class="dropdown-item" href="https://2018.igem.org/Team:Chalmers-Gothenburg/Description" style="color:#16181b">Project Summary</a>
+
         <a class="dropdown-item" href="https://2018.igem.org/Team:Chalmers-Gothenburg/Description" style="color:#16181b">Project Overview</a>
−
         <a class="dropdown-item" href="https://2018.igem.org/Team:Chalmers-Gothenburg/Project" style="color:#16181b">Project</a>
+
         <a class="dropdown-item" href="https://2018.igem.org/Team:Chalmers-Gothenburg/Project" style="color:#16181b">Project Summary</a>
 
         <a class="dropdown-item" href="https://2018.igem.org/Team:Chalmers-Gothenburg/Achievements" style="color:#16181b">Achievements</a>
 
         <a class="dropdown-item" href="https://2018.igem.org/Team:Chalmers-Gothenburg/Achievements" style="color:#16181b">Achievements</a>
 
       </div>
 
       </div>
Line 68: Line 68:
 
       </a>
 
       </a>
 
       <div class="dropdown-menu">
 
       <div class="dropdown-menu">
−
         <a class="dropdown-item" href="https://2018.igem.org/Team:Chalmers-Gothenburg/Attachment" style="color:#16181b">Attachment</a>
+
         <a class="dropdown-item" href="https://2018.igem.org/Team:Chalmers-Gothenburg/Attachment" style="color:#16181b">Binding</a>
 +
        <a class="dropdown-item" href="https://2018.igem.org/Team:Chalmers-Gothenburg/Detection" style="color:#16181b">Detection</a>
 
         <a class="dropdown-item" href="https://2018.igem.org/Team:Chalmers-Gothenburg/Treatment" style="color:#16181b">Treatment</a>
 
         <a class="dropdown-item" href="https://2018.igem.org/Team:Chalmers-Gothenburg/Treatment" style="color:#16181b">Treatment</a>
−
        <a class="dropdown-item" href="https://2018.igem.org/Team:Chalmers-Gothenburg/Detection" style="color:#16181b">Detection</a>
 
 
         <div class="dropdown-divider"></div>
 
         <div class="dropdown-divider"></div>
−
         <a class="dropdown-item" href="https://2018.igem.org/Team:Chalmers-Gothenburg/InterLab" style="color:#16181b">Interlab</a>
+
         <a class="dropdown-item" href="https://2018.igem.org/Team:Chalmers-Gothenburg/InterLab" style="color:#16181b">InterLab</a>
−
         <a class="dropdown-item" href="https://2018.igem.org/Team:Chalmers-Gothenburg/Parts" style="color:#16181b">Biobricks</a>
+
         <a class="dropdown-item" href="https://2018.igem.org/Team:Chalmers-Gothenburg/Parts" style="color:#16181b">BioBricks</a>
 
         <a class="dropdown-item" href="https://2018.igem.org/Team:Chalmers-Gothenburg/Protocols" style="color:#16181b">Protocols</a>
 
         <a class="dropdown-item" href="https://2018.igem.org/Team:Chalmers-Gothenburg/Protocols" style="color:#16181b">Protocols</a>
 
         <a class="dropdown-item" href="https://2018.igem.org/Team:Chalmers-Gothenburg/Safety" style="color:#16181b">Safety</a>
 
         <a class="dropdown-item" href="https://2018.igem.org/Team:Chalmers-Gothenburg/Safety" style="color:#16181b">Safety</a>
Line 79: Line 79:
 
     </li>
 
     </li>
 
     <li class="nav-item dropdown">
 
     <li class="nav-item dropdown">
−
       <a class="nav-link dropdown-toggle" id="navbardrop-model" data-toggle="dropdown">
+
       <a class="nav-link dropdown-toggle" id="navbardrop-hp" data-toggle="dropdown">
−
         MODELING
+
         HUMAN PRACTICES
 
       </a>
 
       </a>
 
       <div class="dropdown-menu">
 
       <div class="dropdown-menu">
−
         <a class="dropdown-item" href="https://2018.igem.org/Team:Chalmers-Gothenburg/Model" style="color:#16181b">Model Overview</a>
+
         <a class="dropdown-item" href="https://2018.igem.org/Team:Chalmers-Gothenburg/Human_Practices" style="color:#16181b">Human Practices</a>
−
         <a class="dropdown-item" href="https://2018.igem.org/Team:Chalmers-Gothenburg/ModelConstruction" style="color:#16181b">Model Design</a>
+
         <a class="dropdown-item" href="https://2018.igem.org/Team:Chalmers-Gothenburg/Collaborations" style="color:#16181b">Collaborations</a>
 +
        <a class="dropdown-item" href="https://2018.igem.org/Team:Chalmers-Gothenburg/Outreach" style="color:#16181b">Outreach</a>
 
       </div>
 
       </div>
 
     </li>
 
     </li>
Line 93: Line 94:
 
   <ul class="navbar-nav align-items-center">
 
   <ul class="navbar-nav align-items-center">
 
     <li class="nav-item dropdown">
 
     <li class="nav-item dropdown">
−
       <a class="nav-link dropdown-toggle" id="navbardrop-hp" data-toggle="dropdown">
+
       <a class="nav-link dropdown-toggle" id="navbardrop-model" data-toggle="dropdown">
−
         HUMAN PRACTICES
+
         MODELING
 
       </a>
 
       </a>
 
       <div class="dropdown-menu">
 
       <div class="dropdown-menu">
−
         <a class="dropdown-item" href="https://2018.igem.org/Team:Chalmers-Gothenburg/Human_Practices" style="color:#16181b">Human Practices</a>
+
         <a class="dropdown-item" href="https://2018.igem.org/Team:Chalmers-Gothenburg/Model" style="color:#16181b">Model Overview</a>
−
         <a class="dropdown-item" href="https://2018.igem.org/Team:Chalmers-Gothenburg/Collaborations" style="color:#16181b">Collaborations</a>
+
         <a class="dropdown-item" href="https://2018.igem.org/Team:Chalmers-Gothenburg/ModelConstruction" style="color:#16181b">Model Design</a>
−
        <a class="dropdown-item" href="https://2018.igem.org/Team:Chalmers-Gothenburg/Outreach" style="color:#16181b">Outreach</a>
+
 
       </div>
 
       </div>
 
     </li>
 
     </li>
Line 161: Line 161:
 
             <a class="anchor" id="Protocols"></a>
 
             <a class="anchor" id="Protocols"></a>
 
             <br>
 
             <br>
−
           
+
<h2> Workflow Overview</h2>
−
 
+
<p> In the figure below an overview of the cloning process, shared by all project parts, is presented.           
 
<figure style="text-align:center;">
 
<figure style="text-align:center;">
−
<img src="https://static.igem.org/mediawiki/2018/1/1f/T--Chalmers-Gothenburg--PlasmidDigestion.png" class="img-fluid">
+
<img src="https://static.igem.org/mediawiki/2018/c/cd/T--Chalmers-Gothenburg--Workflow1.2.png" class="img-fluid" alt="Logo">
−
<figcaption><i><b>Figure 1:</b> Overall approach for the digestion of a plasmid vector</i></figcaption>
+
 
</figure>
 
</figure>
−
 
 
<figure style="text-align:center;">
 
<figure style="text-align:center;">
−
<img src="https://static.igem.org/mediawiki/2018/3/3e/T--Chalmers-Gothenburg--PlasmidAssembly.png" class="img-fluid">
+
<img src="https://static.igem.org/mediawiki/2018/7/7a/T--Chalmers-Gothenburg--Workflow2.2.png" class="img-fluid" alt="Logo">
−
<figcaption><i><b>Figure 2:</b> Overall approach for the assembly of a plasmid and E. coli transformation</i></figcaption>
+
<figcaption><i><b>Figure 1:</b> In this picture a schematic overview of the cloning process is presented, from amplification oof  genes and digestion pasmid to thransformation of plasmid into yeast.</i></figcaption>
 
</figure>
 
</figure>
 +
<br>
 +
 +
 +
 +
       
 +
        <h2>
 +
          Protocols for PCR
 +
        </h2>
 +
 +
        <p class="text">
 +
          <a href="https://static.igem.org/mediawiki/2016/e/e9/T--Chalmers_Gothenburg--Protocols_DreamTaq_PCR.pdf" target="blank">Thermo Scientific DreamTaq DNA Polymerase</a><br>
 +
 +
          <a href="https://static.igem.org/mediawiki/2016/c/c9/T--Chalmers_Gothenburg--Protocols_Phusion_PCR.pdf" target="blank">Thermo Scientific Phusion High-Fidelity DNA Polymerase</a><br>
 +
 +
          <a href="https://static.igem.org/mediawiki/2017/c/c8/T--Chalmers-Gothenburg--Takara_PrimeSTAR_HS_DNA_Polymerase.pdf" target="blank">PrimeSTAR HS DNA Polymerase</a><br>
 +
 +
          <a href="https://static.igem.org/mediawiki/2017/3/36/T--Chalmers-Gothenburg--Fusion_PCR.pdf" target="blank">Fusion PCR</a>
 +
        </p>
 +
 +
 +
        <h2>
 +
          Protocols for purification
 +
        </h2>
 +
 +
        <p class="text">
 +
          <a href="https://static.igem.org/mediawiki/2017/8/81/T--Chalmers-Gothenburg--Thermo_Scientific_GeneJET_PCR_Purification_Kit.pdf" target="blank">Thermo Scientific GeneJET PCR Purification Kit</a><br>
 +
 +
          <a href="https://static.igem.org/mediawiki/2017/8/89/T--Chalmers-Gothenburg--Thermo_Scientific_GeneJET_Gel_Extraction_Kit.pdf" target="blank">Thermo Scientific GeneJET Gel Extraction Kit</a>
 +
        </p>
 +
 +
 +
 +
        <h2>
 +
          Protocols for digestion and assembly
 +
        </h2>
 +
 +
        <p class="text">
 +
          <a href="https://static.igem.org/mediawiki/2017/e/e9/T--Chalmers-Gothenburg--FastDigest_Restriction_Enzymes.pdf" target="blank">FastDigest Restriction Enzymes</a><br>
 +
          <a href="https://static.igem.org/mediawiki/2016/9/9d/T--Chalmers_Gothenburg--Protocols_gibson_assembly.pdf" target="blank">Gibson Assembly Master Mix</a><br>
 +
          <a href="https://static.igem.org/mediawiki/2016/1/1d/T--Chalmers_Gothenburg--Protocols_general_DNA_insert_ligation.pdf" target="blank">DNA Insert Ligation into Vector DNA</a>
 +
        </p>
 +
 +
 +
        <h2>
 +
          Protocols for <i>Saccharomyces cerevisiae</i>
 +
        </h2>
 +
 +
        <p class="text">
 +
          <a href="https://static.igem.org/mediawiki/2017/7/74/T--Chalmers-Gothenburg--Yeast_Transformation_Chemical.pdf" target="blank">Transformation (Chemical)</a><br>
 +
 +
       
 +
 +
          <a href="https://static.igem.org/mediawiki/2017/0/09/T--Chalmers-Gothenburg--Yeast_Transformation-Quick_Version.pdf" target="blank">Transformation (Quick version)</a><br>
 +
 +
          <a href="https://static.igem.org/mediawiki/2016/e/e2/T--Chalmers_Gothenburg--Protocols_S_cerevisiae_quick_genomic_extraction.pdf" target="blank">Isolation of genomic DNA</a>
 +
        </p>
 +
 +
 +
        <h2>
 +
          Protocols for <i>Escherichia coli</i>
 +
        </h2>
 +
 +
        <p class="text">
 +
          <a href="https://static.igem.org/mediawiki/2016/f/f6/T--Chalmers_Gothenburg--Protocols_E_coli_transformation.pdf" target="blank">Transformation (Heat shock)</a><br>
 +
          <a href="https://static.igem.org/mediawiki/2017/b/b1/T--Chalmers-Gothenburg--Thermo_Scientific_GeneJET_Plasmid_Miniprep_Kit.pdf" target="blank">Thermo Scientific GeneJET Plasmid Miniprep Kit</a>
 +
        </p>
 +
 +
 +
        <h2>
 +
          Protocols for Biobricks
 +
        </h2>
 +
 +
        <p class="text">
 +
          <a href="https://static.igem.org/mediawiki/2016/c/c1/T--Chalmers_Gothenburg--Protocols_biobrick_assembly.pdf" target="blank">BioBricks Assembly Protocol</a>
 +
        </p>
 +
 +
 +
        <h2>
 +
          Protocols for Interlab study
 +
        </h2>
 +
 +
        <p class="text">
 +
          <a href="https://static.igem.org/mediawiki/2018/0/09/2018_InterLab_Plate_Reader_Protocol.pdf" target="blank">Interlab study</a>
 +
        </p>
 +
 +
 +
 +
 +
 +
 +
 +
 +
  
−
<figure style="text-align:center;">
 
−
<img src="https://static.igem.org/mediawiki/2018/3/3d/T--Chalmers-Gothenburg--YeastTransformation.png" class="img-fluid">
 
−
<figcaption><i><b>Figure 3:</b> Overall approach for S. cerevisiae transformation</i></figcaption>
 
−
</figure>
 
  
  
Line 188: Line 275:
 
<footer>
 
<footer>
 
   <hr>
 
   <hr>
−
   <h4>
+
   <h4 style="font-size:12pt;">
−
     Contact information
+
     CONTACT INFORMATION:
 
   </h4>
 
   </h4>
 
   <div>
 
   <div>
−
     <p>
+
     <p style="font-size:10pt;">
−
       igemgothenburg@gmail.com   
+
       CHALMERS University of Technology, Gothenburg, Sweden, igemgothenburg@gmail.com   
 +
</p>
 
       <span>
 
       <span>
−
         <a href="https://www.facebook.com/chalmersigem">Facebook</a>   
+
         <a style="font-size:10pt;" href="https://www.facebook.com/chalmersigem">Facebook</a>   
−
         <a href="https://www.instagram.com/chalmersigem">Instagram</a>   
+
         <a style="font-size:10pt;" href="https://www.instagram.com/chalmersigem">Instagram</a>   
−
         <a href="https://twitter.com/igemchalmers">Twitter</a>   
+
         <a style="font-size:10pt;" href="https://twitter.com/igemchalmers">Twitter</a>   
 
       </span>
 
       </span>
−
    </p>
 
 
   </div>
 
   </div>
 
   <br>
 
   <br>

Latest revision as of 19:51, 17 October 2018

Interlab - iGEM Chalmers-Gothenburg 2018

Protocols


Workflow Overview

In the figure below an overview of the cloning process, shared by all project parts, is presented.

Logo
Logo
Figure 1: In this picture a schematic overview of the cloning process is presented, from amplification oof genes and digestion pasmid to thransformation of plasmid into yeast.

Protocols for PCR

Thermo Scientific DreamTaq DNA Polymerase
Thermo Scientific Phusion High-Fidelity DNA Polymerase
PrimeSTAR HS DNA Polymerase
Fusion PCR

Protocols for purification

Thermo Scientific GeneJET PCR Purification Kit
Thermo Scientific GeneJET Gel Extraction Kit

Protocols for digestion and assembly

FastDigest Restriction Enzymes
Gibson Assembly Master Mix
DNA Insert Ligation into Vector DNA

Protocols for Saccharomyces cerevisiae

Transformation (Chemical)
Transformation (Quick version)
Isolation of genomic DNA

Protocols for Escherichia coli

Transformation (Heat shock)
Thermo Scientific GeneJET Plasmid Miniprep Kit

Protocols for Biobricks

BioBricks Assembly Protocol

Protocols for Interlab study

Interlab study